Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate

Sokolovskaya, L. G.; Sigolaeva, Larisa V.; Eremenko, A. V.; Gachok, I. V.; Махаева, Галина Ф.; Strakhova, Nadezda N.; Malygin, V. V.; Richardson, Rudy J.; Kurochkin, Ilya N.

doi:10.1007/s10529-005-0020-1

Cited by 14 publications

(11 citation statements)

References 17 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The tyrosinase electrode was prepared as described below. 39 Graphite/paraffin oil (3.5:1, w/w) was mixed thoroughly and packed firmly into a plastic holder (2 mm diameter). Electrical contact was established by a 0.2-mm copper wire inserted from the rear.…”

Section: Preparation Of Tyrosinase Electrode and Assembly Of Biosensormentioning

confidence: 99%

Biosensor assay of neuropathy target esterase in whole blood as a new approach to OPIDN risk assessment: review of progress

et al. 2007

View full text Add to dashboard Cite

Organophosphates (OPs) that inhibit neuropathy target esterase (NTE) with subsequent ageing can produce OP-induced delayed neuropathy (OPIDN). NTE inhibition in lymphocytes can be used as a biomarker of exposure to neuropathic OPs. An electrochemical method was developed to assay NTE in whole blood. The high sensitivity of the tyrosinase carbon-paste biosensors for the phenol produced by hydrolysis of the substrate, phenyl valerate, allowed NTE activity to be measured in diluted samples of whole blood, which cannot be done using the standard colorimetric assay. The biosensor was used to establish correlations of NTE inhibitions in blood with that in lymphocytes and brain after dosing hens with a neuropathic OP. The results of further studies demonstrated that whole blood NTE is a reliable biomarker of neuropathic OPs for up to 96 hours after exposure. These validation results suggest that the biosensor NTE assay for whole blood could be developed to measure human exposure to neuropathic OPs as a predictor of OPIDN. The small blood volume required (100 μL), simplicity of sample preparation and rapid analysis times indicate that the biosensor should be useful in biomonitoring and epidemiological studies. The present paper is an overview of our previous and ongoing work in this area. Human & Experimental Toxicology (2007) 26, 273-282

show abstract

Section: Preparation Of Tyrosinase Electrode and Assembly Of Biosensormentioning

confidence: 99%

Biosensor assay of neuropathy target esterase in whole blood as a new approach to OPIDN risk assessment: review of progress

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Обнаружение НТЭ в циркулирующих лимфоцитах и тромбоцитах [19][20][21][22] позволило использовать данный фермент в качестве биомаркера воздействия нейропатичных ФОС на животных и человека [22][23][24][25][26][27]. Разработанный нашей группой электрохимический метод определения активности НТЭ с использованием биосенсоров на основе тирозиназы позволил проводить измерения активность НТЭ в цельной крови [28][29][30][31], что невозможно сделать с использованием стандартного колориметрического анализа. Созданный биосенсор был использован для установления корреляций между ингибированием НТЭ в головном мозге, лимфоцитах и крови кур через 24 ч после введения нейропатичного соединения O,O-дипропил-O-дихлорвинилфосфата (diPr-DClVP) [29].…”

Section: Introductionunclassified

Investigation of the Esterase Status as a Complex Biomarker of Exposure to Organophosphorus Compounds

Makhaeva

Рудакова

Richardson

2018

BMCRM

Self Cite

View full text Add to dashboard Cite

Development of biomarkers of human exposures to organophosphorus compounds OPCs and their quantification is a vital component of a system of prediction and early diagnostics of OPC-induced diseases. Our study was focused on investigation of esterase status as a complex biomarker of exposure to OPCs and an aid in accurate diagnosis. We suggest that this complex biomarker should be more effective and informative than standard assays of plasma butyrylcholinesterase (BChE), erythrocyte acetylcholinesterase (RBC AChE), and lymphocyte neuropathy target esterase (NTE). It will help: 1) to assess an exposure as such and to confirm the nonexposure of individuals suspected to have been exposed; 2) to determine if the exposure was to agents expected to produce acute and/or delayed neurotoxicity; 3) to perform dosimetry of the exposure, which provides valuable information for medical treatment. To confirm this hypothesis, we have examined the changes in activity of blood AChE, NTE, BChE and carboxylesterase (CaE) 1 h after i.p. administration of increasing doses of three OPCs with different esterase profiles: the known neuropathic compound O,O-dipropyl-O-dichlorovinyl phosphate (C3H 7O)2P(O)OCH=CCl2 (diPr-DClVP) as the control compound and two model dialkylphosphates (C2H5O)2P(O)OCH(CF3)2 (diEt-PFP) and (C4H9O)2P(O)OCH(CF3)2 (diBu-PFP). The esterases assay was performed in hemolysed blood by spectrophotometric (AChE, BChE, CaE) and biosensor (NTE) methods. Analysis of the obtained dose-dependences for blood esterases inhibition showed that blood BChE and CaE were the most sensitive biomarkers, allowing detection of low doses. Inhibition of blood NTE and AChE can be used to assess the likelihood that an exposure to OPC would produce cholinergic and/or delayed neuropathic effects.

show abstract

“…The discovery of blood NTE in circulating lymphocytes and platelets (Dudek and Richardson, 1982;Richardson and Dudek, 1983;Bertoncin et al, 1985;Maroni and Bleecker, 1986) enabled it to be used as a biomarker of animal and human exposure to neuropathic OP compounds (Lotti, 1986(Lotti, , 1987Lotti et al, 1983Lotti et al, , 1986Richardson and Dudek, 1983;Schwab and Richardson, 1986). Furthermore, the development of sensitive tyrosinase-based biosensors allowed us to measure NTE in whole blood (Sigolaeva et al, 2001;Sokolovskaya et al, 2005). The biosensor was used to establish correlations of NTE inhibitions in whole blood with that in lymphocytes and the brain 24 h after dosing hens with a neuropathic OP compound, O,O-dipropyl-Odichlorvinylphosphate (PrDChVP) (Makhaeva et al, , 2007.…”

Section: Introductionmentioning

confidence: 99%

“…The biosensor was used to establish correlations of NTE inhibitions in whole blood with that in lymphocytes and the brain 24 h after dosing hens with a neuropathic OP compound, O,O-dipropyl-Odichlorvinylphosphate (PrDChVP) (Makhaeva et al, , 2007. These studies indicated that hen whole blood NTE can be assayed and used as a biomarker of exposure to neuropathic OP compounds (Sigolaeva et al, 2001;Makhaeva et al, 2003Makhaeva et al, , 2007Sokolovskaya et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Neuropathy target esterase in mouse whole blood as a biomarker of exposure to neuropathic organophosphorus compounds

et al. 2016

View full text Add to dashboard Cite

The adult hen is the standard animal model for testing organophosphorus (OP) compounds for organophosphorus compound-induced delayed neurotoxicity (OPIDN). Recently, we developed a mouse model for biochemical assessment of the neuropathic potential of OP compounds based on brain neuropathy target esterase (NTE) and acetylcholinesterase (AChE) inhibition. We carried out the present work to further develop the mouse model by testing the hypothesis that whole blood NTE inhibition could be used as a biochemical marker for exposure to neuropathic OP compounds. Because brain NTE and AChE inhibition are biomarkers of OPIDN and acute cholinergic toxicity, respectively, we compared NTE and AChE 20-min IC50 values as well as ED50 values 1 h after single intraperitoneal (i.p.) injections of increasing doses of two neuropathic OP compounds that differed in acute toxicity potency. We found good agreement between the brain and blood for in vitro sensitivity of each enzyme as well for the ratios IC50 (AChE)/IC50 (NTE). Both OP compounds inhibited AChE and NTE in the mouse brain and blood dose-dependently, and brain and blood inhibitions in vivo were well correlated for each enzyme. For both OP compounds, the ratio ED50 (AChE)/ED50 (NTE) in blood corresponded to that in the brain despite the somewhat higher sensitivity of blood enzymes. Thus, our results indicate that mouse blood NTE could serve as a biomarker of exposure to neuropathic OP compounds. Moreover, the data suggest that relative inhibition of blood NTE and AChE provide a way to assess the likelihood that OP compound exposure in a susceptible species would produce cholinergic and/or delayed neuropathic effects. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate

Cited by 14 publications

References 17 publications

Biosensor assay of neuropathy target esterase in whole blood as a new approach to OPIDN risk assessment: review of progress

Biosensor assay of neuropathy target esterase in whole blood as a new approach to OPIDN risk assessment: review of progress

Investigation of the Esterase Status as a Complex Biomarker of Exposure to Organophosphorus Compounds

Neuropathy target esterase in mouse whole blood as a biomarker of exposure to neuropathic organophosphorus compounds

Contact Info

Product

Resources

About