Improved antibacterial efficacy of bacteriophage-cosmetic formulation for treatment of Staphylococcus aureus in vitro

Abo-Elmaaty, Sabah A.; El-Dougdoug, Noha K.; Hazaa, Mahmoud

doi:10.1016/j.aoas.2016.08.002

Cited by 21 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Staphylococcus phages have previously been developed in cosmetic formulations. Phages IS-1 to IS-4 can reduce bacterial growth by 95.45%, compared with free phages and non-supplemented cosmetics, for which the levels are 86.1% and 77%, respectively [24]. In the current study, phage A1 could be combined with the skincare serum, as it showed an ability to reduce the number of SA and SC causing skin diseases in vitro.…”

Section: Cosmetic Formulation Containing Potential Phages Targeting S...mentioning

confidence: 74%

See 1 more Smart Citation

Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp.

Pelyuntha,

Yingkajorn,

Narkpao

et al. 2023

Cosmetics

View full text Add to dashboard Cite

The emergence of multidrug-resistant (MDR) Staphylococcus spp. has resulted in the reduced use of antibiotics in many skincare cosmetic products. Alternative treatments using natural bioactive compounds and chemical agents can be replaced. However, these compounds have induced negative side effects among users and are not environmentally friendly. Phage therapy is an alternative to antibiotics for the treatment of specific pathogenic bacteria including Staphylococcus spp., without harmful effects on human skin cells and microflora. Phages can be potentially used in cosmetic products. The direct application of phage-based cosmetic products on skin can reduce the chance of skin infection caused by pathogenic Staphylococcus spp. In the present work, we isolated 17 Staphylococcus phages from sewage and soil samples. Phage A1 showed the highest lytic ability at 50% (B1 profile), covering 13 tested Staphylococcus isolates including Staphylococcus aureus (SA), methicillin-resistant S. aureus (MRSA), S. capitis (SC), and S. epidermidis (SE). Phage A1 reduced the representative S. aureus ATCC 25923 and S. capitis SC1 by 2.0 ± 0.1 and 4.1 ± 0.3 log units at a multiplicity of infection (MOI) of 104 and by 4.2 ± 0.2 and 4.4 ± 0.5 log units at a MOI of 105 after 6 h of post-phage treatment. The transmission electron microscope revealed that phage A1 was classified in the order Caudovirales of the family Myoviridae based on its appearance. Phage A1 showed optimal survival in the presence of a 0.125% (v/v) solidant DMH suspension after 3 h of post-treatment. Under a phage skincare serum formulation, the titers of phage A1 were reduced by 0.46 and 0.85 log units after storage at 4 and 25 °C, whereas a reduction of 2.96 log units was also observed after storage at 37° for 90 days. This study provides strong evidence for the effectiveness of phage application in cosmetic skincare serum for the treatment of skin diseases caused by MDR and pathogenic Staphylococcus spp. The concept of this study could be advantageous for cosmetic and/or cosmeceutical industries searching for new bioactive ingredients for cosmetic/cosmeceutical products.

show abstract

Section: Cosmetic Formulation Containing Potential Phages Targeting S...mentioning

confidence: 74%

“…They are more efficient in biofilm removal and reducing the staphylococcal count when compared to antibiotics [23]. In addition, phages can be applied in cosmetic formulations for the efficient treatment of skin diseases caused by S. aureus in vitro [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp.

Pelyuntha,

Yingkajorn,

Narkpao

et al. 2023

Cosmetics

View full text Add to dashboard Cite

show abstract

“…In this study, our results showed that the formulated phage could increase the longevity of phages under room temperature. Abo-elmaaty et al (2016) showed that unformulated phage populations were quickly reduced at room temperature after 36 h and 48 h whilst formulated phages decreased in population only after two days. Moreover, Iriarte et al (2007) demonstrated that ɸXV3-16 and ɸXacm 2004-16, formulated with skim milk added with sucrose, survived for 10 days at 28 and 32 °C.…”

Section: Discussionmentioning

confidence: 99%

Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease

Liu¹,

Wang²,

Chia³

et al. 2022

MJM

View full text Add to dashboard Cite

Aims: This study was aimed to evaluate the potential of several carriers to formulate the phages and retain their activity under various pH and temperature conditions. Methodology and results: The skim milk, rice flour, corn flour and CalnuXan (calcium and magnesium) as carriers to formulate the isolated phage to maintain its activity under extreme pH and temperature conditions. Two phages formulated with carriers retained their viability at pH 5, pH 7 and pH 9 compared to that of the unformulated phages. Besides, the formulated phages also retained a high titre compared to the unformulated phages when they were exposed to 37 °C and 45 °C. Based on the in vitro study of the formulation, it was applied in the glass house. The plant height, leaf chlorophyll and disease scoring were recorded and analyzed. In the glass house, the rice plant treated with formulated phages showed higher plant height and chlorophyll content than those treated with unformulated or untreated phages. Nonetheless, both formulated and unformulated protected the rice plant, which showed lower disease severity than the untreated group. Conclusion, significance and impact of study: Phage therapy has been used for treating plant diseases caused by pathogenic bacteria. Despite their effectiveness in killing the pathogen in vitro, the results were not reproducible in the field. Bacteriophages (phages) are sensitive to environmental factors and infection efficiency was dropped when exposed to harmful environments. However, this study successfully formulated two novels Xanthomonas phages, as biocontrol agents against bacterial leaf blight (BLB) disease in rice.

show abstract

“…In another study, Abo-Elmaaty et al (30) isolated a phage from the Myoviridae family and reported its antibacterial and anti-biofilm properties against SA. Jo et al (31) designed a way to show the synergistic antimicrobial effect of phages combined with antibiotics against SA.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Host Range of Lytic Staphylophages on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Boroujeni

Mahzounieh

Kahrizsangi

et al. 2020

Jentashapir J Cell Mol Biol

View full text Add to dashboard Cite

Background: Staphylococcus aureus (S. aureus) is a major cause of nosocomial infections in humans and animals. Because of the widespread resistance to antibiotics, microbiologists are trying to find other therapeutic interventions such as phage therapy for bacterial infections. Objectives: The present study aimed to isolate staphylophages with lytic effects on methicillin-resistant S. aureus (MRSA) clinical isolates as a potential alternative agent to antibiotic therapy. Methods: This experimental, descriptive study is performed in the Microbiology Laboratory of Shahrekord University (Iran) from September 2018 to March 2019. Two cocktails of staphylophages were isolated from Isfahan (Iran) urban sewage samples. The double-layer agar method was used to detect lytic phages. Morphology characteristic by transmission electron microscopy (TEM) images was used to identify staphylophages. One hundred and thirty three S. aureus were isolated from clinical samples of two teaching hospitals in Isfahan and Shiraz, Iran. Methicillin resistance and the presence of the mecA gene were determined by the disk diffusion method and polymerase chain reaction (PCR) assay, respectively. The phage susceptibility of mecA positive isolates was determined by plaque assay. Results: Two staphylophage cocktails were prepared, which had lytic effects on forty-four MRSA isolates. Cocktails 1 and 2 lysed 19 (14.2%) and 25 (18.7%) isolates, respectively. Of 133 S. aureus isolates, 88.7% carried the mecA gene. Conclusions: Different bacteriophages in two phage cocktails had relatively good lytic effects on S. aureus clinical isolates. Therefore, phage cocktails may be an appropriate alternative to antibiotics against S. aureus.

show abstract

Improved antibacterial efficacy of bacteriophage-cosmetic formulation for treatment of Staphylococcus aureus in vitro

Cited by 21 publications

References 18 publications

Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp.

Evaluation of the Effectiveness of Staphylococcus Phages in a Skincare Serum against Staphylococcus spp.

Screening and formulation of novel carriers for Xanthomonas bacteriophage to control bacterial leaf blight disease

Isolation and Host Range of Lytic Staphylophages on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Contact Info

Product

Resources

About