IMpRH Server: an RH mapping server available on the Web

Milan, Denis; Hawken, Rachel; Cabau, Cédric; Leroux, Sophie; Genêt, Carine; Lahbib, Yvette; Tosser, Gwenola; Robic, Annie; Hatey, François; Lee, Alexander; Beattie, Craig W.; Schook, Lawrence B.; Yerle, Martine M.; Gellin, Joël

doi:10.1093/bioinformatics/16.6.558

Cited by 253 publications

(142 citation statements)

References 4 publications

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“…The amplification products were separated on a 2% agarose gel. The PCR results were recorded and analyzed on (http://imprh.toulouse.inra.fr/) for RH mapping (Milan et al, 2000).…”

Section: Radiation Hybrid Mappingmentioning

confidence: 99%

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Cai¹,

Sheng²,

Zhang³

et al. 2011

Genet. Mol. Res.

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“…The amplification products were separated on a 2% agarose gel. The PCR results were recorded and analyzed on (http://imprh.toulouse.inra.fr/) for RH mapping (Milan et al, 2000).…”

Section: Radiation Hybrid Mappingmentioning

confidence: 99%

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Cai¹,

Sheng²,

Zhang³

et al. 2011

Genet. Mol. Res.

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“…DNA (Table 1). Two-point linkage analysis was done using the IMpRH mapping tool available at the IMpRH Web Server (https://www-lgc.toulouse.inra.fr/pig/RH/IMpRH.htm, Milan et al 2000). Association analysis was based on measurements of haemolytic complement activity in the classical and alternative pathway before and after vaccinations and pC8G genotypes obtained from DUMI F 2 animals.…”

Section: Methodsmentioning

confidence: 99%

Detection of a polymorphic site of the porcine <i>C8G</i> gene and evaluation of association with haemolytic complement activity

Ponsuksili

Muráni

et al. 2012

Arch. Anim. Breed.

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C8 is a component of the membrane attack complex (MAC) of the complement system, which causes lysis of the target cells. C8 consists of three subunits C8A, C8B, and C8G. This study focus on the porcine C8G gene aiming to identify its cDNA sequence, to detect single nucleotide polymorphism (SNP), and to analyse its association with haemolytic complement activity in the classical (CH50) and the alternative (AH50) pathway. The C8G is 840 bp in length encoding 202 amino acids. The C8G is similar (≥65 %) among mammalian species (pig, human, cattle, dog, and mouse) at both the cDNA and the protein level. One SNP was detected at nucleotide 423C→T (SNP c.423C>T; codon 124GAC→GAT). The SNP does not segregate among the European commercial breeds German Landrace and Pietrain but in the Vietnamese autochthonous breed Muong Khuong (Vietnamese potbelly pig) and an experimental F 2 crossbreed population based on Duroc and Berlin Miniature Pig (DUMI). Haemolytic complement activity of animals of the DUMI populations obtained at about 6, 14 and 16 weeks of age before and after vaccinations showed short-termed increments due to the immune stimulation and long-term increase due to aging. Family based association tests indicate effects of C8G on AH50 and CH50 at 16 weeks of age immediately before PRRS vaccination (AH50_PRRS0, P=0.087; CH50_PRRS0, P=0.027). However, the results did not indicate a consistent effect of the respective alleles on haemolytic complement activity in the alternative and the classical pathway. The study provides weak evidence for the candidacy of the porcine C8G for innate immune response and disease resistance in pigs.

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“…The PCR reactions were visualized on 10% polyacrylamide/bis-acrylamide 29 : 1 or 2% agarose gels. The results of RH PCR products were analysed with the IMpRH mapping tool accessible through the http:// imprh.toulouse.inra.fr/ web address (Milan et al, 2000).…”

Section: Radiation Hybrid (Rh) Mappingmentioning

confidence: 99%

Polymorphisms in an obesity-related gene (PCSK1) are associated with fat deposition and production traits in Italian heavy pigs

Bertolini

Scotti

Trevisi

et al. 2012

Animal

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The proprotein convertase subtilisin/kexin type 1 (PCSK1) gene encodes the prohormone convertase 1/3 enzyme that processes prohormones into functional hormones that, in turn, regulate central and peripheral energy metabolism. Mutations in the human PCSK1 gene cause severe monogenic obesity or confer risk of obesity. We herein investigated the porcine PCSK1 gene with the aim of identifying polymorphisms associated with fat deposition and production traits in Italian heavy pigs. By re-sequencing about 5.1 kb of this gene in 21 pigs of different breeds, we discovered 14 polymorphisms that were organized in nine haplotypes, clearly distributed in two clades of putative European and Asian origin. Then we re-mapped this gene on porcine chromosome 2 and analysed its expression in several tissues including gastric oxyntic mucosa of weanling pigs in which PCSK1 processes the pre-pro-ghrelin into ghrelin, which in turn is involved in the control of feed intake and energy metabolism. Association analyses between PCSK1 single-nucleotide polymorphisms (SNPs) and production, carcass and several other traits were conducted on five groups of pigs from three different experimental designs, for a total of 1221 animals. Results indicated that the analysed SNPs were associated (P , 0.01 or P , 0.05) with several traits including backfat thickness and visible intermuscular fat in Italian Duroc (ID) and growth performances in Italian Large White (ILW) and in ILW 3 Italian Landrace pigs. However, the effects estimated in the ILW were opposite to the effects reported in the ID pigs. Suggestive association (P , 0.10) was observed with muscle cathepsin B activity, opening, if confirmed, potential applications to reduce the excessive softness defect of the green hams that is of particular concern for the processing industry. The results obtained supported the need to further investigate the PCSK1 gene to fully exploit the value of its variability and apply this information in pig breeding programmes.

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IMpRH Server: an RH mapping server available on the Web

Cited by 253 publications

References 4 publications

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Detection of a polymorphic site of the porcine <i>C8G</i> gene and evaluation of association with haemolytic complement activity

Polymorphisms in an obesity-related gene (PCSK1) are associated with fat deposition and production traits in Italian heavy pigs

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IMpRH Server: an RH mapping server available on the Web

Cited by 253 publications

References 4 publications

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Mapping, expression and regulation of the TRα gene in porcine adipose tissue

Detection of a polymorphic site of the porcine &lt;i&gt;C8G&lt;/i&gt; gene and evaluation of association with haemolytic complement activity

Polymorphisms in an obesity-related gene (PCSK1) are associated with fat deposition and production traits in Italian heavy pigs

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Detection of a polymorphic site of the porcine <i>C8G</i> gene and evaluation of association with haemolytic complement activity