Importance of the cutoff ratio for detecting antibodies against hepatitis A virus in oral fluids by enzyme immunoassay

Tourinho, Renata Santos; Amado, Luciane Almeida; Villar, Lívia Melo; Sampaio, Daniela Vieira; Moraes, Alyne Costa; Ó, Kicia Maria Rodrigues do; Gaspar, Ana; Paula, Vanessa Salete de

doi:10.1016/j.jviromet.2011.01.014

Cited by 16 publications

(18 citation statements)

References 24 publications

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“…The calculation of the cut‐off value was also an important step for assay optimization when saliva samples were used because commercial EIAs have been developed for sera samples. In the present study, three methods were evaluated, and the AUROC analysis method provided the best sensitivity as was reported for anti‐HAV (34) and HBsAg detection in saliva samples (25). This method is an important tool to classify continuous variables, such as OD values, in categorical variables, such as positive and negative results.…”

Section: Discussionmentioning

confidence: 91%

An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti‐HCV)

Cruz

Marques

Villela-Nogueira

et al. 2012

J Oral Pathology Medicine

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J Oral Pathol Med (2012) 41: 793–800 Background: Saliva samples can be used as an alternative fluid for against hepatitis C virus (anti‐HCV) detection owing to the ease of collection and excellent acceptability. This study was conducted to optimize a commercial enzyme immunoassay (EIA) to detect anti‐HCV in saliva samples. Methods: Ninety‐six individuals donated paired serum and saliva samples that were obtained, using a commercial device (Salivette) and spitting into a sterile container. Initially, elution buffer for the Salivette samples, sample volume, incubation time and temperature, and two different anti‐HCV EIAs were evaluated. Using the optimized assay, three methods for cut‐off calculation were also evaluated. Results: A 20‐fold increase in the sample volume for both collection methods was needed. Moreover, the Radim assay was the most appropriate assay for anti‐HCV detection in saliva samples, and the quality parameters were increased when a ROC curve was used to determine the cut‐off value. Using this optimized assay, the sensitivities, specificities, accuracies, positive and negative predictive values were above 90% for saliva obtained using both the Salivette and spitting methods. Using this assay, discordant false‐negative results were obtained for only two Salivette samples and five spitting samples. The concordance kappa was 93% for the Salivette method and 86.1% for the spitting method, demonstrating excellent performance. Conclusions: Saliva samples obtained for both methods can be employed for anti‐HCV detection among HCV‐infected or HCV‐suspected cases, but several modifications must be performed on commercial EIAs to obtain good results. Moreover, samples obtained with commercial devices are more appropriate for anti‐HCV detection in saliva samples.

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Section: Discussionmentioning

confidence: 91%

An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti‐HCV)

Cruz

Marques

Villela-Nogueira

et al. 2012

J Oral Pathology Medicine

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“…In MA city almost half of the participants reported that urine could diagnose viral hepatitis. Viral hepatitis markers are detected in urine and other biological fluids such as saliva, semen, breast milk, pancreatic secretions, bile and vaginal, tears, menstrual, pleural, and nasopharyngeal [ 9 , 24 ], but the efficiency of detection in fluids like urine is not ideal for diagnosis of all types of hepatitis viruses [ 25 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

A Cross-Sectional Study of Viral Hepatitis Perception among Residents from Southeast and North Regions of Brazil

Cruz

Paula

Villar

2018

IJERPH

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Few data are available regarding viral hepatitis perception among the general global population. The present study aims to estimate the perception of viral hepatitis in a cohort of individuals living in two geographical regions of Brazil: North (Manaus city (MA)) and Southeast (Rio de Janeiro city (RJ)). A cross-sectional, descriptive study was carried out among 287 subjects recruited in MA (134) and RJ (153). All individuals answered a questionnaire assessing socio-demographic characteristics and viral hepatitis awareness. Participants’ responses were scored and divided using interquartile values. Associations between socio-demographic characteristics and knowledge were also evaluated. Interquartile analysis scored 0–21 correct answers as “Very Weak”; 22–27 as “Weak”; 28–31 as “Intermediate”; and 32–47 as “Desirable”. Mean ± standard deviations (SD) of correct responses were weak in both MA (24.1 ± 7.0) and RJ (26.3 ± 7.3). Bivariate analysis showed an association between viral hepatitis awareness and both education level (p < 0.001) and family income (p < 0.01). Desirable scores were more common in female participants (61%), those aged between 21–30 years (40%), those with a secondary education (51.7%), those who received high income (31.6%), and those from RJ (70.0%). Health education campaigns in these cities are recommended to increase knowledge and reduce the transmission of these viruses.

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“…Cut‐off value evaluation for anti‐HCV detection from DBS samples was important because commercial EIAs have not been developed for these samples. In this study, the ROC curve was employed for both EIAs, similar to previous studies for HAV and HBV detection from saliva samples [Cruz et al, 2011; Tourinho et al, 2011]. Croom et al [2006] used the cut‐off value established by the manufacturer, and McCarron et al [1999], Parker et al [1999] and Judd et al [2003] employed other CO values for testing DBS samples for anti‐HCV.…”

Section: Discussionmentioning

confidence: 99%

Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

Marques

Brandão

Silva

et al. 2012

Journal of Medical Virology

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This study was undertaken to optimize and compare the efficiency of two commercial EIAs for anti-HCV detection (HCV Ab Radim, Pomezzia, Italy and ETI-AB-HCVK-4 DiaSorin, Vercelli, Italy), in dried blood spot (DBS) samples. The long-term stability of anti-HCV on DBS samples stored at three environmental conditions was also evaluated at: 2-8 °C, 20-25 °C, and -20 °C. Paired DBS and serum samples were obtained from individuals with or without anti-HCV. The type of elution buffer, sample and conjugate volume, sample incubation time and cut-off values were evaluated. For both EIAs, a larger sample volume was used, and the cut-off value determined by the manufacturer was employed for Radim EIA; however, ROC curve analysis was used for the DiaSorin EIA. The sensitivity and specificity of Radim EIA on DBS were 97.5% and 99.5%, respectively, and of DiaSorin EIA were 88.9% and 98.9%, respectively. Accurate results were obtained for a period of 117 days using DBS samples stored at all storage conditions, but storage at -20 °C resulted in the lowest variation among the absorbance values. Both EIAs demonstrated the same limit of detection (until dilution of 1:10(4) with estimated viral load of 3.1 × 10(-1) UI/ml), but the Radim EIA was associated with the best performance because a low coefficient of variation was observed in the repetition and reproducibility studies. In conclusion, commercial EIAs can be optimized for anti-HCV detection in DBS samples that are extremely stable at different conditions for more than 100 days.

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Importance of the cutoff ratio for detecting antibodies against hepatitis A virus in oral fluids by enzyme immunoassay

Cited by 16 publications

References 24 publications

An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti‐HCV)

An evaluation of different saliva collection methods for detection of antibodies against hepatitis C virus (anti‐HCV)

A Cross-Sectional Study of Viral Hepatitis Perception among Residents from Southeast and North Regions of Brazil

Dried blood spot samples: Optimization of commercial EIAs for hepatitis C antibody detection and stability under different storage conditions

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