Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes

Perreault, Sally D.; Barbee, Randy R.; Slott, Valerie L.

doi:10.1016/0012-1606(88)90070-x

Cited by 298 publications

(219 citation statements)

References 27 publications

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“…4A, pretreatment with BDNF increased by 2-and 5-fold the proportions of MII oocytes that developed into the two-cell and blastocyst-stage embryos, respectively. Because the oocyte content of glutathione is important to sperm nuclear decondensation (13), the glutathione concentration in oocytes also was evaluated. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Kawamura

Mulders

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

168

126

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Optimal development of fertilized eggs into preimplantation embryos is essential for reproduction. Although mammalian oocytes ovulated after luteinizing hormone (LH) stimulation can be fertilized and promoted into early embryos in vitro, little is known about ovarian factors important for the conditioning of eggs for early embryo development. Because LH interacts only with ovarian somatic cells, its potential regulation of oocyte functions is presumably mediated by local paracrine factors. We performed DNA microarray analyses of ovarian transcripts and identified brainderived neurotrophic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the preovulatory LH surge. Ovarian BDNF acts on TrkB receptors expressed exclusively in oocytes to enhance first polar body extrusion of oocytes and to promote the in vitro development of zygotes into preimplantation embryos. Furthermore, in vivo treatment with a Trk receptor inhibitor suppressed first polar body extrusion and the progression of zygotes into blastocysts. Thus, ovarian BDNF is important to nuclear and cytoplasmic maturation of the oocyte, which is essential for successful oocyte development into preimplantation embryos. Treatment with BDNF could condition the cultured oocytes for optimal progression into the totipotent blastocysts.early embryo development ͉ gonadotropins ͉ ovulation I n vertebrates, rupture of ovarian follicles and final maturation of oocytes occur in response to stimulation by pituitaryderived luteinizing hormones (LH) that act on the somatic granulosa and theca cells surrounding the oocyte. Shortly after stimulation by the preovulatory surge of LH, oocytes arrested at the late prophase resume meiosis characterized by germinal vesicle (nuclear envelope) breakdown (GVBD), chromosome condensation, and extrusion of the first polar body in preparation for fertilization and early embryonic development. Recent studies demonstrated that the endocrine hormone LH stimulates ovarian production of EGF-like factors from granulosa cells and insulin-like 3 from theca cells to promote GVBD (1, 2). In addition to nuclear maturation exemplified by GVBD and extrusion of the first polar body to complete the first meiotic division, oocytes also undergo cytoplasmic maturation characterized by cytoplasmic changes essential for monospermic fertilization, processing of the sperm, and preparation for development to preimplantation embryos (3, 4). Although the spermatozoon provides an essential element for embryo generation, the developmental fate of the embryo is principally dictated by the oocyte. However, few studies have explored ovarian factors that may be important for the conditioning of the oocyte in preparation for fertilization and preimplantation development.Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of proteins known to activate the highaffinity TrkB receptor and the pan-neurotrophin low-affinity receptor p75 (5). Although neurotrophins are widely expressed in the central nervous syst...

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Section: Resultsmentioning

confidence: 99%

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Kawamura

Mulders

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

168

126

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“…These substances have been reported to be readily inactivated or fail to be produced when eggs are matured in vitro under suboptimal conditions [33]. Recently, it has been reported that glutathione is important in the acquisition and maintenance of sperm nuclear decondensing activity in the hamster oocytes [34]. Further studies will be needed to decide changes in the levels and functions of the egg cytoplasmic factors during maternal aging.…”

Section: Abnormalities In Fertilizationmentioning

confidence: 99%

Delay in Ovulation and Fertilization and Asynchronous Pronuclear Development in Aged Hamsters.

Suzuki

Moriguchi

Kida

et al. 1996

Journal of Reproduction and Development

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“…Prepubertal goat IVM-IVF-oocytes show a low incidence of male pronuclear (MPN) formation [10] and a high percentage of haploid embryos (59% of 2-4 cell embryos; [11]). The transformation of the fertilising sperm head into the MPN has been shown to be positively related to intracellular glutathione levels (GSH; [12]). De Matos et al [13,14], have shown that the addition of cysteamine to the maturation medium increases the GSH concentration in cow oocytes and improves the in vitro blastocyst yield.…”

Section: Introductionmentioning

confidence: 99%

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

et al. 2003

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-The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 µM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB-(unstained oocytes). The oocytes were matured in a conventional IVM medium supplemented with cysteamine 100 µM. The control group consisted of oocytes not exposed to BCB and matured without cysteamine. The IVM-oocytes were inseminated and cultured in synthetic oviductal fluid (SOF) for 7 days. The normal fertilisation rate (oocytes showing 2 pronuclei and 1 sperm tail) of BCB+ oocytes (40%) was higher than those of BCB-(21%) and control oocytes (22%). The percentage of morulae plus blastocysts was higher (P < 0.05) in the BCB+ group than in the BCB-group (23.8 vs. 5.1%, respectively). In conclusion, the integration of the BCB test and the addition of cysteamine in the protocol of in vitro embryo production from prepubertal goat oocytes has improved the developmental rates of embryo development. embryo / IVF / IVM / thiol

show abstract

Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes

Cited by 298 publications

References 27 publications

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Delay in Ovulation and Fertilization and Asynchronous Pronuclear Development in Aged Hamsters.

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

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