Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils

Michiu, Delia; Socaciu, Maria-Ioana; Fogarasi, Melinda; Jimborean, Anamaria Mirela; Ranga, Floricuţa; Mureșan, Vlad; Semeniuc, Cristina Anamaria

doi:10.3390/molecules27041345

Cited by 20 publications

(17 citation statements)

References 34 publications

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“…The Folin–Ciocalteu method was used to estimate the TPC in crude extract samples. 39 In brief, 1 mL of 2 N Folin–Ciocalteu phenol reagent was mixed with 1 mL of 1 mg/mL plant extract (prepared by dissolving it in methanol), and then the mixture was diluted by the addition of 5 mL distilled water. After incubating for 5 minutes, 1 mL of 10% Na 2 CO 3 solution was added, and then the mixture was incubated for 1 hour in the dark at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Lamichhane¹,

Sharma²,

Sapkota³

et al. 2023

JEP

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Introduction Herbal products have been widely used for the treatment of diseases throughout the ages. In this research, we investigated antioxidant, antibacterial, anti-adipogenic, and anti-inflammatory activities of methanolic extracts of five ethnomedicinally important plants; namely, Alnus nepalensis , Dryopteris sparsa , Artocarpus lacucha , Litsea monopetala , and Lyonia ovalifolia . Methods We investigated the DPPH free radical scavenging potential, sensitivity of selected bacterial strains towards the extracts using a disc diffusion assay, anti-inflammatory activity in RAW-264.7 cells, and anti-adipogenic activity by the ORO assay in 3T3-L1 preadipocytes. Results and discussion The extract of A. nepalensis showed significant antioxidant activity (IC 50 =4.838 µ g/mL), followed by A. lacucha , L. monopetala , and L. ovalifolia , exhibiting comparable IC 50 values to that of ascorbic acid (IC 50 =5.063 µ g/mL). Alnus nepalensis also showed good antibacterial activity in disc diffusion methods, with remarkable zones of inhibition in A. baumannii (14.66 mm) and P. mirabilis (15.50 mm) bacterial species. In addition, A. nepalensis was found to increase adipogenesis in 3T3-L1 cells, evidenced by increased lipid deposition in differentiated 3T3-L1 cells. A similar pattern of increased adipogenesis was observed on treatment with L. ovalifolia extracts. On the other hand, A. lacucha effectively reduced lipid deposition in 3T3-L1 cells at 100 µ g/mL (75.18±6.42%) by inhibiting adipogenesis, showing its potential use in the management of obesity. Furthermore, A. lacucha 100 µg/mL (15.91±0.277 µ M) and L. monopetala 75 µ g/mL (12.52±0.05 µ M) and 100 µg/mL (11.77±0.33 µ M) significantly inhibited LPS-induced nitric oxide production in RAW 264.7 cells. Also, A. nepalensis and L. ovalifolia inhibited NO production significantly, endorsing their anti-inflammatory potential. Conclusion The findings from these in-vitro studies suggest that the selected five plants possess remarkable antioxidant, antibacterial, anti-adipogenic, and anti-inflammatory activities. This study opens the door to conduct further advanced in-vivo experiments to find possible le...

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Section: Methodsmentioning

confidence: 99%

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Lamichhane¹,

Sharma²,

Sapkota³

et al. 2023

JEP

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“…The measures were acquired 20 min after the addition of sodium carbonate, using a Shimadzu UV-1800 spectrophotometer (Tokyo, Japan) with a measuring wavelength of 760 nm. The absorbance of all the samples was compared to a previously prepared calibration curve of gallic acid and the results were expressed in milligrams of gallic acid per gram of sample (mg GAE/g) [ 85 , 86 ].…”

Section: Methodsmentioning

confidence: 99%

Stabilization of Anthocyanins from Coffee (Coffea arabica L.) Husks and In Vivo Evaluation of Their Antioxidant Activity

et al. 2023

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Coffee (Coffea arabica L.) is one of the most popular and widely consumed products throughout the world, mainly due to its taste, aroma, caffeine content, and natural antioxidants. Among those antioxidants, anthocyanins are one of the most important natural pigments, which can be found in coffee husks. It is widely known that anthocyanins have multiple health benefits partially linked to their antioxidant properties. However, anthocyanins have low stability and are sensitive to all types of changes. In order to prevent its degradation, anthocyanins can be stabilized with nanoparticles. Thus, the main objective of this study was to evaluate the stability of the anthocyanins extracted from coffee husks, using three different extracting agents (ethanol, methanol, and water) and stabilizing them through conjugation with zinc oxide nanoparticles. The anthocyanins extracts were mainly composed of cyanidin-3-rutinoside (97%) and the total phenolic compounds of the fresh extracts were 458.97 ± 11.32 (methanol), 373.53 ± 12.74 (ethanol), and 369.85 ± 15.93 (water) mg GAE/g. On the other hand, the total phenolic compounds of the nanoparticle–anthocyanin conjugates underwent no significant changes after stabilization as the major loss was less than 3%. Furthermore, the percentage of anthocyanins’ degradation was less than 5% after 12 weeks of storage. On top of that, fresh anthocyanin extracts and anthocyanin–nanoparticle conjugates exhibited a strong protective effect against oxidative stress and increased the survival rate of Caenorhabditis elegans.

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“…= LOD m 3.29 where σ is the standard error of the predicted y-values for each x value in a regression line, and m is the slope of the calibration plot. 40…”

Section: Preparation Of Urolithin Standard Solutionsmentioning

confidence: 99%

Portable Smartphone-Assisted Graphene Quantum Dots Sensing Platform for the Detection of Gut Microbial Metabolites

Lim,

Huang,

Ambrosi

et al. 2024

ACS Appl. Nano Mater.

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Metabolomics research is gaining traction lately as there is a growing interest in studying the global relationship between human diseases and the metabolite network. The presence and concentration of beneficial gut-derived metabolites depends on the individual's microbiota composition and diet, with direct metabolite-based supplementation being an appealing option for those unable to produce them. This work serves as an approach in the direct detection of gut-derived metabolites through a smartphone-based approach, as a faster and cheaper alternative to more traditional techniques such as chromatography or the study of gut bacteria culture. Urolithin, a beneficial gut microbial metabolite, was successfully detected with our developed smartphonebased optical assay with low limits of detection of 0.257−0.422 μM that were comparable to those measured with a fluorescence spectrophotometer or even better than some reported HPLC methods. On top of a good sensitive detection with a reduced analysis time, the proposed "turn off-to-on" fluorescence sensing strategy that employed graphene quantum dots coupled with copper(II) ions also distinguished urolithin metabotypes and exhibited good selectivity in the presence of other reducing compounds and phenolic analogues. Our developed approach has demonstrated its versatility in direct gut-derived metabolite detection in commercial dietary supplements, making it a user-friendly tool for food and pharmaceutical product analysis.

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Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils

Cited by 20 publications

References 34 publications

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Screening of Antioxidant, Antibacterial, Anti-Adipogenic, and Anti-Inflammatory Activities of Five Selected Medicinal Plants of Nepal

Stabilization of Anthocyanins from Coffee (Coffea arabica L.) Husks and In Vivo Evaluation of Their Antioxidant Activity

Portable Smartphone-Assisted Graphene Quantum Dots Sensing Platform for the Detection of Gut Microbial Metabolites

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