Implantation of an Injectable Bone Substitute Material Enables Integration Following the Principles of Guided Bone Regeneration

Barbeck, Mike; Jung, Ole; Smeets, Ralf; Gosau, Martin; Schnettler, R.; Rider, Patrick; Houshmand, Alireza; Koržinskas, Tadas

doi:10.21873/invivo.11808

Cited by 22 publications

(29 citation statements)

References 42 publications

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“…At 60 days post implantationem, only remnants of the former jellyfish collagen scaffolds were observable, resulting in a cell-and vessel-rich connective tissue. Thereby, the jellyfish collagen scaffolds were gradually degraded from their periphery towards the scaffold center as also observed in the case of other biomaterials such as injectable bone substitute materials [45][46][47][48]. In comparison, the matrices based on porcine pericardium were stable up to 60 days, and interestingly, the inflammatory response associated with these matrices at day 10 and 30 was nearly completely decreased up to this latest study time point.…”

Section: Discussionsupporting

confidence: 58%

“…The described integration behavior providing a gradual cell invasion and degradation has also shown to optimally interact with the bony integration process in the case of different bone pastes [45][46][47]. Bone regenerative properties of bone pastes are proved in both preclinical and clinical studies and it could thus be assumed that the jellyfish collagen scaffolds may be suitable for the same application [45][46][47]. However, it was described that the bone pastes serve as a scaffold-like structure up to 6 months after implantation.…”

Section: Discussionmentioning

confidence: 99%

“…Three slides of every tissue explant were used for histochemical staining (Hematoxylin and Eosin (H&E), Movat's pentachrome and Masson's trichrome). The staining was performed following previously described methods [44,45,83].…”

Section: Sample Preparation and Staining Proceduresmentioning

confidence: 99%

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In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Flaig¹,

Radenković

Najman

et al. 2020

IJMS

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Jellyfish collagen, which can be defined as “collagen type 0” due to its homogeneity to the mammalian types I, II, III, V, and IX and its batch-to-batch consistent producibility, is of special interest for different medical applications related to (bone) tissue regeneration as an alternative to mammalian collagen-based biomaterials. However, no in vivo studies regarding the induction of M1- and M2-macrophages and their time-dependent ration as well as the analysis of the bone regeneration capacity of jellyfish collagen scaffolds have been conducted until now. Thus, the goal of this study was to determine the nature of the immune response to jellyfish collagen scaffolds and their bone healing capacities. Two in vivo studies using established implantation models, i.e., the subcutaneous and the calvarian implantation model in Wistar rats, were conducted. Furthermore, specialized histological, histopathological, and histomorphometrical methods have been used. As a control biomaterial, a collagen scaffold, originating from porcine pericardium, which has already been stated as biocompatible, was used for the subcutaneous study. The results of the present study show that jellyfish collagen scaffolds are nearly completely resorbed until day 60 post implantation by stepwise integration within the subcutaneous connective tissue mediated mainly by macrophages and single multinucleated giant cells. Interestingly, the degradation process ended in a vessel rich connective tissue that is understood to be an optimal basis for tissue regeneration. The study results showed an overall weaker immune response to jellyfish collagen than to porcine pericardium matrices by the induction of significantly lower numbers of macrophages together with a more balanced occurrence of M1- and M2-macrophages. However, both collagen-based biomaterials induced balanced numbers of both macrophage subtypes, which supports their good biocompatibility. Moreover, the histomorphometrical results for the calvarial implantation of the jellyfish scaffolds revealed an average of 46.20% de novo bone formation at day 60, which was significantly higher compared to the control group. Thereby, the jellyfish collagen scaffolds induced also significantly higher numbers of anti-inflammatory macrophages within the bony implantation beds. Altogether, the results show that the jellyfish collagen scaffolds allowed for a directed integration behavior, which is assumed to be in accordance with the concept of Guided Bone Regeneration (GBR). Furthermore, the jellyfish collagen scaffolds induced a long-term anti-inflammatory macrophage response and an optimal vascularization pattern within their implant beds, thus showing excellent biocompatibility and (bone) tissue healing properties.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Flaig¹,

Radenković

Najman

et al. 2020

IJMS

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“…Previous study of ISBG showed that it is easy to handle and can serve as an excellent BMP9 carrier in order to demonstrate its in vitro osteoinductive potential [ 32 ]. Another in vivo study showed that ISBG was gradually invaded by cells and complex tissue elements [ 26 ]. Here it was observed that ISBG can build a barrier-like structure and with that was able to control the soft tissue influx into the implantation bed.…”

Section: Discussionmentioning

confidence: 99%

“…Even though the addition of a third synthetic phase (HA nanoparticles in a waterbased gel) seems to promote higher volumetric bone loss after three years, it still enables complete recovery of the bone defects and excellent volume stability over time [24,25]. This injectable synthetic bone graft (ISBG) (60% HA/40% ß-TCP granules and HA nanoparticles within water-based gel) can also build a barrier-like structure that is able to control soft tissue infiltration into the implanted bed [26]. For that reason, we assessed the histological outcome of GBR with such ISBG in patients having extensive chronic apical periodontitis that caused buccal fenestration.…”

Section: Introductionmentioning

confidence: 99%

Histological and Radiological Features of a Four-Phase Injectable Synthetic Bone Graft in Guided Bone Regeneration: A Case Report

Čandrlić

Kačarević

Ivanišević

et al. 2020

IJERPH

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Background and objective: Injectable synthetic bone grafts (ISBG) are widely used biomaterials for regeneration purposes. The aim of this case report was to examine the efficacy of ISBG in the management of buccal fenestration in the case of a 25-year-old female. Case report: After a traumatic tooth extraction, the defect was filled with ISBG and covered with a resorbable membrane. The ISBG showed easy handling and the patient had no complications during healing. Six months after augmentation, a bone biopsy was taken during implant bed preparation. The histological results showed good integration of ISBG into the newly formed bone and no signs of tissue inflammation. Additionally, a CBCT (cone beam computed tomography) analysis was performed to support the histological results. Conclusion: The use of the examined ISBG led to successful treatment of the buccal fenestration defect.

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Evaluation of Injectable Composite Material Comprising Biphasic Bone Substitutes and Crosslinked Collagen

et al. 2023

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Bone tissue engineering has emerged as a promising approach to regenerate bone tissue, and injectable biomaterials have shown potential for bone regeneration applications due to their ease of administration and ability to fill irregularly shaped defects. This study aims to develop and characterize an injectable composite material comprising biphasic bone substitutes (BBS) and crosslinked porcine collagen type I for bone regeneration applications. The collagen is crosslinked via a UVA‐riboflavin crosslinking strategy and evaluated by testing the physicochemical properties, including the rheological behavior, dynamic storage modulus (G′) and loss modulus (G″), and in vitro degradation process. The results show that the crosslinked collagen (xCol) exhibits suitable physicochemical properties for injectability and improved viscoelasticity and degradation resistance. Furthermore, xCol is then combined with BBS in a predetermined ratio, obtaining the injectable composite material. The biocompatibility of the materials is evaluated in vitro by XTT and BrdU assays on fibroblasts and preosteoblasts. The results demonstrate that the composite material is biocompatible and supporting pre‐osteoblasts proliferation. In conclusion, the injectable composite material BBS‐xCol has promising physiochemical and biological properties for bone regeneration applications. Further studies are warranted to evaluate its efficacy in vivo and optimize its composition for clinical translation.

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Implantation of an Injectable Bone Substitute Material Enables Integration Following the Principles of Guided Bone Regeneration

Cited by 22 publications

References 42 publications

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Histological and Radiological Features of a Four-Phase Injectable Synthetic Bone Graft in Guided Bone Regeneration: A Case Report

Evaluation of Injectable Composite Material Comprising Biphasic Bone Substitutes and Crosslinked Collagen

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