Imparting cell adhesion to poly(vinyl alcohol) hydrogel by coating with hydroxyapatite thin film

Hayami, Takashi; Matsumura, Kazuaki; Kusünoki, Masanobu; Nishikawa, Hiroaki; Hontsu, Shigeki

doi:10.1016/j.matlet.2006.10.019

Cited by 31 publications

(25 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was expected as PVA-Tyr is a synthetic polymer with no biological components incorporated to support cell attachment. Previous studies have also confirmed that cells do not attach to hydrogels made out of pure PVA [48,49]. The incorporation of gelatin at concentrations as low as 0.01% markedly increased the bioactivity of the hydrogels, with approximately 60% seeding efficiency achieved.…”

Section: L929 Cell Adhesion Studiesmentioning

confidence: 76%

Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels

et al. 2013

View full text Add to dashboard Cite

Section: L929 Cell Adhesion Studiesmentioning

confidence: 76%

Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels

et al. 2013

View full text Add to dashboard Cite

“…In general, NIH3T3 cell line has relatively shorter DT than L929 cell line and thus the baseline metabolism was increased. [28][29][30][31][32] Direct cytotoxicity evaluation. Figure 6 shows the direct cytotoxicity test results of different materials on L929 cell and NIH3T3 cell.…”

Section: Cytotoxicity Evaluationmentioning

confidence: 99%

In vitro study on Zr‐based bulk metallic glasses as potential biomaterials

et al. 2010

View full text Add to dashboard Cite

With pure Ti and pure Zr as controls, the corrosion resistance, ion release behavior, and in vitro biocompatibility of Be-containing Zr₄₁Ti₁₄Cu₁₂Ni₁₀Be₂₃ bulk metallic glass (BMG) (LM1), Zr₄₄Ti₁₁Cu₁₀Ni₁₀Be₂₅ BMG (LM1b), and Be-free Zr₅₇Nb₅Cu₁₅.₄Ni₁₂.₆Al₁₀ BMG (LM106) were investigated in terms of electrochemical measurements in simulated body fluid (SBF) with pH value 7.4 and artificial saliva (AS) with pH value 6.3, and 3-[4,4-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay using L929 and NIH3T3 cells, aiming to assess the feasibility of Zr-based BMGs as potential biomaterial. It was found that LM1b showed superior corrosion resistance to LM106 and LM1 in both SBF and AS, and comparable with pure Ti and pure Zr. After 7200 s immersion, a two-layer structure oxide film was formed on LM1, LM1b, and pure Zr surfaces, while one-layer structure oxide film was formed on LM106 and pure Ti surfaces. The pitting corrosion potentials of LM1b were much higher than that of LM1, LM106, and pure Zr, resulting in very few ions releasing into the electrolytes. No Be ion could be detected but a little amount of Cu ion was detected for LM106, LM1, and LM1b after immersion in Dulbecco's modified Eagle's medium for 72 h at 37 °C. The indirect cytotoxicity results show that LM106, LM1, and LM1b extracts had no cytotoxicity to L929 and NIH3T3 cells. The direct cytotoxicity results show that cells could adhere well on the Zr-based BMG surface as in pure Ti and Zr. Lower cell proliferation rate of LM106 and LM1 is observed when compared with LM1b, which was found to be caused by Cu ion releasing rather than by Be ion.

show abstract

“…For instance, the incorporation of osteoconductive hydroxyapatite particles into PVA hydrogel films has been shown to enhance cell adhesion to PVA hydrogels and stimulate osteoblast proliferation (30,31). As an illustration, coatings of hydroxyapatite particles were produced by swelling-induced gelation on the same spherical PAAc-PAAm substrates as the ones studied above.…”

Section: Application Potential Of Hydrogel Film Fabrication By Swellimentioning

confidence: 99%

Hydrogel films and coatings by swelling-induced gelation

Moreau

Chauvet

François

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Hydrogel films used as membranes or coatings are essential components of devices interfaced with biological systems. Their design is greatly challenged by the need to find mild synthesis and processing conditions that preserve their biocompatibility and the integrity of encapsulated compounds. Here, we report an approach to produce hydrogel films spontaneously in aqueous polymer solutions. This method uses the solvent depletion created at the surface of swelling polymer substrates to induce the gelation of a thin layer of polymer solution. Using a biocompatible polymer that self-assembles at high concentration [poly(vinyl alcohol)], hydrogel films were produced within minutes to hours with thicknesses ranging from tens to hundreds of micrometers. A simple model and numerical simulations of mass transport during swelling capture the experiments and predict how film growth depends on the solution composition, substrate geometry, and swelling properties. The versatility of the approach was verified with a variety of swelling substrates and hydrogel-forming solutions. We also demonstrate the potential of this technique by incorporating other solutes such as inorganic particles to fabricate ceramic-hydrogel coatings for bone anchoring and cells to fabricate cell-laden membranes for cell culture or tissue engineering. H ydrogel films are used in a number of biomedical applications like wound healing (1), drug delivery (1), antiadhesive membranes (2), soft-tissue reconstruction (3, 4), and cell culture (5). The quality of their interactions with biological tissues or entrapped cells is central to the performance of these systems. Although considerable advances have been made in synthesis (6) and processing (7) to tailor these interactions, the toxicity of by-products and the degradation caused by harsh processing conditions remain key issues (8), which can be overcome (9) but are often addressed at the cost of difficult synthesis or multistep processes. There is a great interest for simple, versatile, and in vivo-friendly methods to fabricate and modify hydrogels. We propose a strategy to design hydrogel films in mild conditions. This method could be used to produce freestanding hydrogel membranes or hydrogel coatings to functionalize other hydrogels.Our approach relies on the understanding of transport phenomena occurring near the surface of a polymer network swelling in a polymer solution. As an illustration, let us consider a flat polymer network with thickness H 0 immersed in a solution containing a volume fraction Φ 0 of free polymer chains, as depicted in Fig. 1A. When the solvent of the solution is also a solvent for the network, the network swells in a diffusive process. For early immersion times t, the increase in thickness ΔH is given by the following (10, 11):where H ∞ is the thickness at equilibrium swelling in the polymer solution and D 0 is a diffusivity coefficient depending on the dynamics of the network and of the solution. The systems of interest are those where the chains in solution do not penetrate...

show abstract

Imparting cell adhesion to poly(vinyl alcohol) hydrogel by coating with hydroxyapatite thin film

Cited by 31 publications

References 10 publications

Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels

Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels

In vitro study on Zr‐based bulk metallic glasses as potential biomaterials

Hydrogel films and coatings by swelling-induced gelation

Contact Info

Product

Resources

About