Impact of Titanium Dioxide Nanoparticle Dispersion State and Dispersion Method on Their Toxicity Towards A549 Lung Cells and &lt;I&gt;Escherichia&lt;/I&gt; &lt;I&gt;coli&lt;/I&gt; Bacteria

Carrière, Marie; Pigeot‐Rémy, S.; Casanova, Axelle; Dhawan, Alok; Lazzaroni, Jean-Claude; Guillard, C.; Herlin‐Boime, Nathalie

doi:10.1166/jtt.2014.1010

Cited by 4 publications

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“…The frequency distance histogram of all the direct techniques reveals a multimodal size distribution (Figure 8D) with a major mode at 22 nm while minor modes can be observed at 8 or 32 nm. Similar to the TiO 2 A12 and likely due to agglomeration, DLS average value (35 ± 5 nm, [68]) is above every other techniques. The amplitude percentage variation for direct techniques is 40%, whereas it is 77% for all techniques.…”

Section: Resultsmentioning

confidence: 81%

“…Therefore, their size increases/decreases upon the agglomeration/disruption status of a given NP. Several studies focus on sample preparation and sample homogenization, where many efforts are targeted to the dispersion problem of NPs [29,33], including in nanotoxicology studies [68], and encouraging results have been found for specific NPs [43,44,45,82]. Although methods exist to model multimodal distribution of NPs using light scattering and SAXS techniques [83], a recent study reveals that the observation of multimodal dispersion of isobutylcyanoacrylate-based NPs only succeeded by AFM, nanoparticle tracking analysis, and tunable resistive pulse sensing techniques [33], whereas no light scattering batch methods could reproduce the particle size distribution.…”

Section: Discussionmentioning

confidence: 99%

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On the Operational Aspects of Measuring Nanoparticle Sizes

et al. 2018

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Nanoparticles are defined as elementary particles with a size between 1 and 100 nm for at least 50% (in number). They can be made from natural materials, or manufactured. Due to their small sizes, novel toxicological issues are raised and thus determining the accurate size of these nanoparticles is a major challenge. In this study, we performed an intercomparison experiment with the goal to measure sizes of several nanoparticles, in a first step, calibrated beads and monodispersed SiO2 Ludox®, and, in a second step, nanoparticles (NPs) of toxicological interest, such as Silver NM-300 K and PVP-coated Ag NPs, Titanium dioxide A12, P25(Degussa), and E171(A), using commonly available laboratory techniques such as transmission electron microscopy, scanning electron microscopy, small-angle X-ray scattering, dynamic light scattering, wet scanning transmission electron microscopy (and its dry state, STEM) and atomic force microscopy. With monomodal distributed NPs (polystyrene beads and SiO2 Ludox®), all tested techniques provide a global size value amplitude within 25% from each other, whereas on multimodal distributed NPs (Ag and TiO2) the inter-technique variation in size values reaches 300%. Our results highlight several pitfalls of NP size measurements such as operational aspects, which are unexpected consequences in the choice of experimental protocols. It reinforces the idea that averaging the NP size from different biophysical techniques (and experimental protocols) is more robust than focusing on repetitions of a single technique. Besides, when characterizing a heterogeneous NP in size, a size distribution is more informative than a simple average value. This work emphasizes the need for nanotoxicologists (and regulatory agencies) to test a large panel of different techniques before making a choice for the most appropriate technique(s)/protocol(s) to characterize a peculiar NP.

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Section: Resultsmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

On the Operational Aspects of Measuring Nanoparticle Sizes

et al. 2018

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“…The preparation of suspensions was done using a several steps method detailed elsewhere ( Carrière et al, 2013 ). A stock solution was prepared using a one hundred mg of TiO 2 material manually mixed with 10 ml of sterile filtered water.…”

Section: Methodsmentioning

confidence: 99%

TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Becker

Schroecksnadel

Geisler

et al. 2014

Food and Chemical Toxicology

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Assessment of the in vitro genotoxicity of TiO₂ nanoparticles in a regulatory context

et al. 2018

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A review of in vitro genotoxicity studies on titanium dioxide nanoparticles (TiO-NPs) published between 2010 and 2016 was performed by France in the framework of the CLP Regulation 1272/2008/EC. Neither the few in vivo studies of low quality nor the larger number of acceptable in vitro studies available for genotoxicity allowed France to conclude on the genotoxicity of TiO-NPs. Based on this work, it was decided to compare the acceptable in vitro studies to understand the reasons for the diverging results observed, such as the materials tested or of the protocols used and their inherent interferences. The systematic review performed on in vitro genotoxicity data for TiO-NPs was then restricted to studies with the highest level of confidence among studies following OECD guidelines and the largely applied comet assay. Indeed, the aim of this article is to understand why, even if judged of good quality, the 36 publications selected and analyzed did not lead to a clear picture. Some recommendations to be taken into account before performing new in vitro genotoxicity assays for insoluble particles such as TiO-NPs are proposed. Although secondary genotoxic effects consequent to oxidative stress seem to be the major mechanism responsible for the genotoxicity of TiO-NPs reported in some studies, primary genotoxic effects cannot be excluded. Further studies are needed to clarify the exact mode of action of TiO-NPs and to highlight which physicochemical properties lead to their genotoxicity in vitro to ultimately identify a specific combination of parameters that could represent a risk in vivo.

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Impact of Titanium Dioxide Nanoparticle Dispersion State and Dispersion Method on Their Toxicity Towards A549 Lung Cells and <I>Escherichia</I> <I>coli</I> Bacteria

Cited by 4 publications

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On the Operational Aspects of Measuring Nanoparticle Sizes

On the Operational Aspects of Measuring Nanoparticle Sizes

TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Assessment of the in vitro genotoxicity of TiO₂ nanoparticles in a regulatory context

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Impact of Titanium Dioxide Nanoparticle Dispersion State and Dispersion Method on Their Toxicity Towards A549 Lung Cells and <I>Escherichia</I> <I>coli</I> Bacteria

Cited by 4 publications

References 0 publications

On the Operational Aspects of Measuring Nanoparticle Sizes

On the Operational Aspects of Measuring Nanoparticle Sizes

TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Assessment of the in vitro genotoxicity of TiO2 nanoparticles in a regulatory context

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Assessment of the in vitro genotoxicity of TiO₂ nanoparticles in a regulatory context