Impact of Sucrose Concentrations on in vitro Pollen Germination of Okra, Hibiscus esculentus

Baloch, Mohammed Jurial; Lakho, Abdul Rahim; Bhutto, Hidayatullah; Solangi, Mohammed Yousuf

doi:10.3923/pjbs.2001.402.403

Cited by 16 publications

(5 citation statements)

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“…It is widely accepted that pollen from different plant species may require a specific amount of sucrose in an artificial medium for germination in vitro. This exogenous sucrose requirement has been found to play an important role as a nutritive resource and osmoregulator for pollen germination 12,15,16 . The germination percentage of pollen from yellowish orange increased with the increasing concentrations of sucrose in the medium, suggesting that endogenous physiological differences of pollen might also play a role in regulation of pollen germination.…”

Section: Discussionmentioning

confidence: 99%

The utility of electrospun nanofibre mats for in vitro germination of Artabotrys hexapetalus pollen

Bodhipadma¹,

Noichinda²,

Chanunpanich³

et al. 2016

ScienceAsia

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This study investigates the influence of electrospun fibre mats on in vitro pollen germination of Artabotrys hexapetalus (L.f.) Bhandari (synonym: A. odoratissimus R. Brown). There was no difference in the size of the pollen from two closely related stages of flower development, namely the yellow florets and yellowish orange florets. On agarbased medium supplemented with 20% sucrose, about 50% of the pollen from the yellowish orange florets germinated but only 25% of those from the yellow florets did. More pollen from the yellowish orange florets germinated in liquid medium than on agar-gelled medium but pollen tube elongation was greater in agar-gelled medium. Electrospinning of synthetic polymer solutions of polyvinylidene fluoride (PVDF) or polylactic acid (PLA) was used to generate mats composed of nonwoven (randomly oriented) nanofibres as well as those composed of a mixture of nonwoven and aligned (PVDF4 and PLA4) nanofibres. Compared with liquid, agar medium, and other types of nanofibre mats, PVDF4 was found to be the best physical support substrate for germination of the pollen from the yellowish orange florets (70%) and tube growth (about 400% increase over that in liquid medium). Pollen germination was largely inhibited on all four types of 18 µm thick nanofibre mats but not on the 8 µm thick ones.

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Section: Discussionmentioning

confidence: 99%

The utility of electrospun nanofibre mats for in vitro germination of Artabotrys hexapetalus pollen

Bodhipadma¹,

Noichinda²,

Chanunpanich³

et al. 2016

ScienceAsia

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“…As for the roles of the most frequent PGM components, sucrose serves as an effective energy source and an osmoticum for PG in vitro (Heslop-Harrison and Heslop-Harrison, 1992;Rodriguez-Enriquez et al, 2013;Selinski and Scheibe, 2014;Reinders, 2016). Pollen bursting and failure to germinate in vitro is often associated with inadequate sucrose concentrations (Baloch et al, 2001). The mineral elements boron and calcium have also been found to play several critical regulatory and structural functions in PG.…”

Section: Resultsmentioning

confidence: 99%

A Compendium of in vitro Germination Media for Pollen Research

Tushabe

Rosbakh

2021

Front. Plant Sci.

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The correct choice of in vitro pollen germination media (PGM) is crucial in basic and applied pollen research. However, the methodological gaps (e.g., strong focus of current research on model species and cultivated plants along with the lack of general rules for developing a PGM) makes experimenting with pollen difficult. We closed these gaps by compiling a compendium of optimized in vitro PGM recipes from more than 1800 articles published in English, German, and Russian from 1926 to 2019. The compendium includes 1572 PGM recipes successfully used to germinate pollen grains or produce pollen tubes in 816 species representing 412 genera and 114 families (both monocots and dicots). Among the 110 components recorded from the different PGM recipes, sucrose (89% of species), H3BO3 (77%), Ca2+ (59%), Mg2+ (44%), and K+ (39%) were the most commonly used PGM components. PGM pH was reported in 35% of all studies reviewed. Also, we identified some general rules for creating PGM for various groups of species differing in area of research (wild and cultivated species), phylogenetic relatedness (angiosperms vs. gymnosperms, dicots vs. monocots), pollen physiology (bi- and tri-cellular), biochemistry (starchy vs. starchless pollen grains), and stigma properties (dry vs. wet), and compared the component requirements. Sucrose, calcium, and magnesium concentrations were significantly different across most categories indicating that pollen sensitivity to sugar and mineral requirements in PGM is highly group-specific and should be accounted for when composing new PGM. This compendium is an important data resource on PGM and can facilitate future pollen research.

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“…Under natural conditions, pollen of different species often require specific media for germination [14,15]. In this study, the germinating medium of Karni and Aloni [16] with modifications by Reddy and Kakani [17] was used since it gave positive results to the other ginger species examined by Mendez et al [18].…”

Section: In Vitro Pollen Germinationmentioning

confidence: 99%

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

Mendez

Acma²

2018

J.Trop.Life.Science

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Zingiberaceous plants are predominantly propagated through underground rhizomes and seeds. In this study, the viability of pollen, rate of pollen germination and length of pollen tube of the Philippine endemic Hornstedtia conoidea were examined. Four petri plates containing pollen samples were prepared, of which two petri plates were used immediately after the collection, while the other two petri plates were stored for one week. The determination of pollen viability was made employing stain tests using IKI (iodine + potassium iodide) solution and safranin and measured immediately after the collection and after one week. Likewise, pollen germination test was carried out in vitro on agar medium and measured after 24 hours and one week of incubation. Data revealed that pollen viability was higher in the samples which were sown immediately with safranin with percentage pollen viability of 92.43%, while IKI test was lower with 89.36%. On the other hand, the pollen stored for one week measured 47.29% for safranin test and 33.14% for IKI test. The percentage germination of pollen after 24 hours was 72.65%, while 11.13% after one week. The pollen samples which were subjected for tube growth were examined for two weeks and gave positive results. Overall, it can be concluded that H. conoidea pollen can successfully be collected and stored for certain time. Knowledge regarding the pollen viability, pollen germination and pollen tube growth of this species will give practical benefit for plant breeding and conservation purposes, since H. conoidea is found in the wild, and as of now, the species has been recorded only in the Philippines.

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Impact of Sucrose Concentrations on in vitro Pollen Germination of Okra, Hibiscus esculentus

Cited by 16 publications

References 0 publications

The utility of electrospun nanofibre mats for in vitro germination of Artabotrys hexapetalus pollen

The utility of electrospun nanofibre mats for in vitro germination of Artabotrys hexapetalus pollen

A Compendium of in vitro Germination Media for Pollen Research

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

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