Whilst developing a fertilisation assay to quantify the onset of fertilisability in oocytes from cultured Nereis virens, development through to the blastula stage was observed within the negative control. In the absence of sperm, some oocytes re-initiated the cell cycle without fertilisation which is the usual cue in this species. Investigations showed that a lower pH of 6.6 significantly increased the incidence of this parthenogenetic development which was also observed to be more rapid than was observed in …
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