Impact of Nitrate and Ammonium Concentrations on Co-Culturing of Tetradesmus obliquus IS2 with Variovorax paradoxus IS1 as Revealed by Phenotypic Responses

Perera, Isiri Adhiwarie; Abinandan, Sudharsanam; Subashchandrabose, Suresh R.; Venkateswarlu, Kadiyala; Naidu, Ravi; Megharaj, Mallavarapu

doi:10.1007/s00248-021-01832-6

Cited by 6 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, pH could be an important factor that determines the bioavailability of metals in microalgae at biologically excess concentrations. A perusal of the literature indicates that microalgae exhibit phenotypic response through changes in physiological and metabolic signature molecules such as carbohydrates, proteins, and lipids for their growth in any environmental cues ( Charles et al., 2019 ; Abinandan et al., 2020a ; ( Perera et al., 2021b , Perera et al., 2021c )). Metals at higher concentrations that are biologically in excess may trigger certain undesirable effects on the growth of microalgae ( Abinandan et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic changes in microalgae at acidic pH mediate their tolerance to higher concentrations of transition metals

Abinandan

Venkateswarlu

Megharaj

2021

Current Research in Microbial Sciences

Self Cite

View full text Add to dashboard Cite

Highlights Acid-tolerant microalgae were grown at pH 3.5 and 6.7 in presence of heavy metals (HMs). HMs-induced phenotypic changes in microalgae were evaluated by ATR-FTIR spectroscopy. Higher HMs bioavailability affected microalgae more at pH 6.7 than pH 3.5. Acclimation of microalgal strains to acidic pH significantly alleviates HMs toxicity.

show abstract

Section: Introductionmentioning

confidence: 99%

Phenotypic changes in microalgae at acidic pH mediate their tolerance to higher concentrations of transition metals

Abinandan

Venkateswarlu

Megharaj

2021

Current Research in Microbial Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Synergistic bacterial associations with microalgae are known to exist, where vitamins from bacteria are provided for the algae in exchange for organic carbon [13,14]. An increased bacterial load over the cultivation period in co-cultures has been frequently observed [34,44]. Changes in community compositions in bacteria associated with microalgae in response to nitrogen fluctuations in the environment are known [52], as well as designed nitrogen source variations affecting the bacterial composition in algae-bacterial consortia [53].…”

Section: Discussionmentioning

confidence: 99%

“…This is also reflected in the total counts on day 3 for the three conditions (ANOVA, p < 0.001). Both the type and concentration of the nitrogen source have been shown to influence the bacterial load in co-cultures [44]. Changes in the nutrient supply appear to significantly perturb the cohabiting bacterial composition of the algal culture (ANOVA, p < 0.001).…”

Section: Changes In Nutrient Supply Increase Cohabiting Bacterial Loa...mentioning

confidence: 99%

Density and Composition of Cohabiting Bacteria in Chlorella vulgaris CCAP 211/21A Is Influenced by Changes in Nutrient Supply

Almalki,

Nwokeoji,

Vaidyanathan

2024

SynBio

View full text Add to dashboard Cite

Microalgae have considerable potential as a renewable feedstock for biochemical and bioethanol production that can be employed in processes associated with carbon capture. Large-scale microalgae cultivations are often non-axenic and are often cohabited by bacteria. A better understanding of the influence of cohabiting bacteria on microalgae productivity is required to develop sustainable synthetic co-culture processes at scale. Nutrient limitation is a frequently employed strategy in algal cultivations to accumulate energy reserves, such as lipids and carbohydrates. Here, a non-axenic culture of an estuarine green microalga, Chlorella vulgaris CCAP 211/21A, was studied under nutrient replete and deplete conditions to assess how changes in nutrient supply influenced the cohabiting bacterial population and its association with intracellular carbohydrate accumulations in the alga. Nutrient limitation resulted in a maximum carbohydrate yield of 47%, which was 74% higher than that in nutrient replete conditions. However, the latter condition elicited a 2-fold higher carbohydrate productivity. Three cohabiting bacterial isolates were cultivable from the three culture conditions tested. These isolates were identified using the 16S rRNA gene sequence to belong to Halomonas sp. and Muricauda sp. The composition of the bacterial population varied significantly between the growth conditions and time points. In all cases and at all time points, the dominant species was Halomonas isolates. Nutrient depletion resulted in an apparent loss of Muricauda sp. This finding demonstrates that nutrient supply can be used to control cohabiting bacterial populations in algal cultures, which will enable the development of synthetic co-culture strategies for improving algae productivity.

show abstract

“…The genus Variovorax was also abundantly detected in the five P. bursaria strains with poor proliferation ( Supplementary Table S2 ). It has also been detected in root nodules of plants and is known as a plant proliferation-promoting bacterium with nitrogen-fixing abilities ( Jiang et al, 2012 ; Satola et al, 2013 ; Finkel et al, 2020 ; Perera et al, 2022 ). It would be interesting if symbiotic algae can escape from P. bursaria strains with poor proliferation into the culture medium and receive support such as nitrogen fixation from the genus Variovorax .…”

Section: Discussionmentioning

confidence: 99%

Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation

Himi

Miyoshi‐Akiyama²,

Matsushima³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The ciliate Paramecium bursaria harbors several hundred symbiotic algae in its cell and is widely used as an experimental model for studying symbiosis between eukaryotic cells. Currently, various types of bacteria and eukaryotic microorganisms are used as food for culturing P. bursaria; thus, the cultivation conditions are not uniform among researchers. To unify cultivation conditions, we established cloned, unfed strains that can be cultured using only sterile medium without exogenous food. The proliferation of these unfed strains was suppressed in the presence of antibiotics, suggesting that bacteria are required for the proliferation of the unfed strains. Indeed, several kinds of bacteria, such as Burkholderiales, Rhizobiales, Rhodospirillales, and Sphingomonadales, which are able to fix atmospheric nitrogen and/or degrade chemical pollutants, were detected in the unfed strains. The genetic background of the individually cloned, unfed strains were the same, but the proliferation curves of the individual P. bursaria strains were very diverse. Therefore, we selected multiple actively and poorly proliferating individual strains and compared the bacterial composition among the individual strains using 16S rDNA sequencing. The results showed that the bacterial composition among actively proliferating P. bursaria strains was highly homologous but different to poorly proliferating strains. Using unfed strains, the cultivation conditions applied in different laboratories can be unified, and symbiosis research on P. bursaria will make great progress.

show abstract

Impact of Nitrate and Ammonium Concentrations on Co-Culturing of Tetradesmus obliquus IS2 with Variovorax paradoxus IS1 as Revealed by Phenotypic Responses

Cited by 6 publications

References 48 publications

Phenotypic changes in microalgae at acidic pH mediate their tolerance to higher concentrations of transition metals

Phenotypic changes in microalgae at acidic pH mediate their tolerance to higher concentrations of transition metals

Density and Composition of Cohabiting Bacteria in Chlorella vulgaris CCAP 211/21A Is Influenced by Changes in Nutrient Supply

Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation

Contact Info

Product

Resources

About