Impact of l-carnitine on lipid content and post thaw survivability of buffalo embryos produced in vitro

Verma, Megha; Pandey, Sriti; Bhat, Irfan Ahmad; Mukesh, B.; Anand, J. C.; Chandra, Vikash; Sharma, G. Taru

doi:10.1016/j.cryobiol.2018.04.001

Cited by 22 publications

(19 citation statements)

References 28 publications

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“…However, we did not evaluate the expression of OCNT2. We found no effect of l-carnitine on the relative expression of dgat1 and dgat2 genes, where, in contrast to the result reported by Ghanem et al [23], Verma et al [30] found a significant reduction of dgat1 and dgat2 gene expression using l-carnitine.…”

Section: Discussioncontrasting

confidence: 98%

“…Held-Hoeker and colleagues showed that 2.5 mM l-carnitine added in SOF medium during in vitro culture significantly improved the post-warming hatching rate of bovine embryos [16]. Verma et al found that adding 1.5 or 2 mM l-carnitine on mSOF medium (without FBS) during in vitro culture, significantly improved the post-thawing survival rate in buffalo embryos [30]. The discrepancies between these studies in terms of the effects of l-carnitine supplementation on embryonic cryotolerance are likely due to the differences in terms of l-carnitine concentrations, the molecular weight of the l-carnitine used, the culture media used, and the presence or absence of several macromolecules, including FBS and BSA [17].…”

Section: Discussionmentioning

confidence: 99%

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l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

Carrillo-González

Rodríguez-Osorio

Long

et al. 2020

IJMS

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l-carnitine is a potent antioxidant used for in vitro culture systems. Controversial results have been reported using l-carnitine in culture medium at different stages of in vitro bovine embryo production. Cumulus-oocyte complexes (n = 843) were in vitro-fertilized and cultured and added (treatment group) or not added (control group) with l-carnitine. At day three of culture, each group was subdivided into two subgroups receiving no l-carnitine (group 1), 3.8 mM l-carnitine added during in vitro maturation (group 2), 1.5 mM added during the in vitro culture (group 3), and 3.8 mM and 1.5 mM added during the maturation and culture, respectively (group 4). At day 8, blastocyst embryos were examined for mitochondrial activity, the presence of lipid droplets, total cell number, gene expression, and cryotolerance by vitrification. The data were analyzed with a one-way analysis of variance. l-carnitine added in the late in vitro culture significantly reduced mitochondrial activity and lipid content, and upregulated ifn-τ and ptgs2 gene expression compared to controls (p < 0.05). l-carnitine supplementation did not significantly affect the embryo rate production or survival rate after vitrification and warming (p > 0.05). l-carnitine supplementation significantly improved embryo potential to develop viable pregnancies in agreement with a study reporting improved pregnancy rates.

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionmentioning

confidence: 99%

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

Carrillo-González

Rodríguez-Osorio

Long

et al. 2020

IJMS

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“…The proteomic analysis demonstrated the upregulation of proteins involved in the degradation of lipids and cholesterol biosynthesis in embryos cultured under physiological oxygen. Simultaneously, the fluorescent intensity of lipids, an indicator of the density of lipid droplets [63], was low in embryos cultured under low oxygen. Here, we suggested that the better cryo-survival ability of the embryos under 5% O2 may be related to the changes in lipid metabolism, ending in a lower content of lipid droplets.…”

Section: Modification In the Lipid Composition Might Be Associated Wimentioning

confidence: 99%

“…These findings are supported by the low lipid fluorescent intensity in embryos under physiological oxygen. The fluorescent intensity is an indicator of density of lipid droplets [63]. The higher lipid level is attributed to the poor cryo-survival ability of embryos [62].…”

Section: Modification In the Lipid Composition Might Be Associated Wimentioning

confidence: 99%

Proteomics Analysis Reveals that Warburg Effect along with Modification in Lipid Metabolism Improves In Vitro Embryo Development under Low Oxygen

Shahzad

Wadood

et al. 2020

IJMS

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The molecular mechanism regulating embryo development under reduced oxygen tension remains elusive. This study aimed to identify the molecular mechanism impacting embryo development under low oxygen conditions. Buffalo embryos were cultured under 5% or 20% oxygen and were evaluated according to their morphological parameters related to embryo development. The protein profiles of these embryos were compared using iTRAQ-based quantitative proteomics. Physiological O2 (5%) significantly promoted blastocyst yield, hatching rate, embryo quality and cell count as compared to atmospheric O2 (20%). The embryos in the 5% O2 group had an improved hatching rate of cryopreserved blastocysts post-warming (p < 0.05). Comparative proteome profiles of hatched blastocysts cultured under 5% vs. 20% O2 levels identified 43 differentially expressed proteins (DEPs). Functional analysis indicated that DEPs were mainly associated with glycolysis, fatty acid degradation, inositol phosphate metabolism and terpenoid backbone synthesis. Our results suggest that embryos under physiological oxygen had greater developmental potential due to the pronounced Warburg Effect (aerobic glycolysis). Moreover, our proteomic data suggested that higher lipid degradation, an elevated cholesterol level and a higher unsaturated to saturated fatty acid ratio might be involved in the better cryo-survival ability reported in embryos cultured under low oxygen. These data provide new information on the early embryo protein repertoire and general molecular mechanisms of embryo development under varying oxygen levels.

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“…lipids (Verma et al, 2018), gene expression patterns (Lonergan, Fair, Corcoran, & Evans, 2006) and on modifications in epigenetic markers, which can continue after birth too (Ramos-Ibeas et al, 2019).…”

mentioning

confidence: 99%

Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo (Bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo

Sharma

Nath

Prasad

et al. 2020

Reprod Domestic Animals

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It is commonly accepted that in vivo-derived (IVD) embryos are developmentally superior in quality than in vitro-produced (IVP) embryos (Sudano et al., 2013). The in vitro environment directly influences the embryonic phenotypes and results. Mimicking in vivo environment is very difficult, and somehow, we generally failed in this task. Unbalanced in vitro culture conditions itself impact directly embryo competence, since it has a significant effect on the cellular metabolism (D'Souza et al., 2018), quality and quantity of

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Impact of l-carnitine on lipid content and post thaw survivability of buffalo embryos produced in vitro

Cited by 22 publications

References 28 publications

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

l-Carnitine Supplementation during In Vitro Maturation and In Vitro Culture Does not Affect the Survival Rates after Vitrification and Warming but Alters Inf-T and ptgs2 Gene Expression

Proteomics Analysis Reveals that Warburg Effect along with Modification in Lipid Metabolism Improves In Vitro Embryo Development under Low Oxygen

Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo (Bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo

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