Impact of Inflammation on the Osteoblast in Rheumatic Diseases

Baum, Rebecca; Gravallese, Ellen M.

doi:10.1007/s11914-013-0183-y

Cited by 112 publications

(86 citation statements)

References 80 publications

(83 reference statements)

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“…Although much information regarding RA focuses on the effects of inflammation on osteoclast differentiation and function, recent studies have begun to elucidate the impact of proinflammatory cytokines, such as OPN, on the osteoblast (22,23). Studies regarding the inflammatory development of RA show that OSM potentially contributes to monocyte migration and macrophage activation in synovial tissue (24,25).…”

Section: Opn Increases Osm Production In Human Osteoblastsmentioning

confidence: 99%

Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy

Chiang

Huang

et al. 2015

The Journal of Immunology

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Accumulating evidence indicates that subchondral bone might play an essential role in rheumatoid arthritis (RA). Osteopontin (OPN) induces the production of an important proinflammatory cytokine involved in the pathogenesis of RA. This study evaluated the activation of oncostatin M (OSM) by OPN in human primary osteoblasts to understand RA pathogenesis and characterized the intracellular signaling pathways involved in this activation. Quantitative PCR, ELISA, and Western blot results indicated that stimulation of human primary osteoblasts with OPN induces OSM expression through αvβ3 integrin/c-Src/platelet-derived growth factor receptor transactivation/MEK/ERK. Treatment of osteoblasts with OPN also increased c-Jun phosphorylation, AP-1 luciferase activity, and c-Jun binding to the AP-1 element on the OSM promoter, as demonstrated using chromatin immunoprecipitation assay. Moreover, inhibition of OPN expression using lentiviral-OPN short hairpin RNA resulted in the amelioration of articular swelling, cartilage erosion, and OSM expression in the ankle joint of mice with collagen-induced arthritis as shown using microcomputed tomography and immunohistochemistry staining. Our results imply that OSM expression in osteoblasts increases in response to OPN-induced inflammation in vitro. Finally, lentiviral-OPN short hairpin RNA ameliorates the inflammatory response and bone destruction in mice with collagen-induced arthritis. Therefore, OPN may be a potential therapeutic target for RA.

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Section: Opn Increases Osm Production In Human Osteoblastsmentioning

confidence: 99%

Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy

Chiang

Huang

et al. 2015

The Journal of Immunology

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“…Proinflammatory cytokines stimulate the osteoclast differentiation and function. 15 This mechanism may account for the statistically significant correlation between water and calcium concentrations that were observed. Spectral CT could thus demonstrate bone marrow oedema in the sclerotic part of the bone, while the STIR images remain insensitive in this regard.…”

Section: Discussionmentioning

confidence: 95%

Comparing the diagnostic utility of sacroiliac spectral CT and MRI in axial spondyloarthritis

Zhang

Guo

et al. 2016

BJR

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Objective: To compare the clinical value of sacroiliac spectral CT and MRI in diagnosing axial spondyloarthritis (SpA). Methods: 137 patients with low back pain and suspected axial SpA were recruited. 76 patients were diagnosed with axial SpA, and 49 patients were diagnosed with nonspecific low back pain (nLBP). Each patient underwent spectral CT and MRI examinations of the sacroiliac joints. Water-and calcium-based material decomposition images were reconstructed for quantitative analysis. The marrowto-muscle ratios of water and calcium concentrations and short tau inversion recovery (STIR) signal intensity, as well as Hounsfield units in the ilium and sacrum were compared between nLBP and patients with axial SpA. Results: Spectral CT is comparable with MRI for the detection of bone marrow oedema, and it is superior to MRI for detection of osseous sclerosis and erosions. MRI is superior to spectral CT in detecting enthesitis and synovitis. There were statistically significant differences in STIR signal intensity, water concentration and calcium concentration ratios as well as CT values between nLBP and patients with axial SpA (p , 0.05) in the ilium. There was a statistically significant but weak correlation between ratios of water concentration and STIR signal intensity in both the ilium and sacrum (p , 0.05). Overall, the iliac water concentration was most sensitive for detection of patients with SpA. The positive likelihood ratio of the STIR signal intensity ratio was the highest. The diagnostic odds ratio of the calcium concentration ratio was the highest, and its negative likelihood ratio was the lowest. Conclusion: Spectral CT not only depicts findings of chronic sacroiliitis (i.e. bone erosion and sclerosis), but also can detect and quantify the extent of marrow oedema in patients with SpA with activity sacroiliitis. The sensitivity of MRI for diagnosis of early synovitis and enthesitis remains superior. The combination of spectral CT and MRI may thus improve diagnostic accuracy in the diagnosis of axial SpA. Advances in knowledge: Spectral CT can measure both calcium and water concentration of the sacroiliac joints. The combination of spectral CT and MRI may thus improve diagnostic accuracy in the diagnosis of axial SpA.

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“…RA'da periartiküler ve sistemik osteoporoz normal popülasyondan daha fazladır (30). FCAA'nın RA'ya bağlı osteoporozu belirlemede uygun bir model olduğu önceki çalışmalarla gösterilmiştir (30,(40)(41)(42) (30,46). Gerek bu, gerekse önceki çalışmalarda ozon uygulamasının özellikle serum TNF-α düzeylerini olumlu etkilediği gösterilmiştir (13,14,38,39).…”

Section: Discussionunclassified

The Effect of Ozone on Bone Strenght in Animal Model of Rheumatoid Arthritis

Bozbaş¹,

Kilimci²,

Yılmaz³

et al. 2016

Tod

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SummaryObjective: Periarticular and systemic osteoporosis are more common in rheumatoid arthritis (RA) than normal population. In this study, we aimed to investigate the effectiveness of ozone on bone strength in Freund's complete adjuvant (FCA) arthritis, which is considered as the animal model for RA. Materials and Methods: In this study, 28 male Wistar rats were used. Saline was injected into the hindpaws of 14 rats, and Freund's complete adjuvant was injected into the hindpaws of the other 14 rats, subcutaneously. At the end of two weeks, 40 µg/ml ozone was administered intraperitoneally to 7 of the rats in each group for 6 times totally within duration of three weeks. At the 6 th week, serum interleukin-1 (IL-1), IL-6 and tumor necrosis factor-alpha (TNF-α) levels were measured. Right femurs were separated for 3-point flexure test. Results: TNF-α levels of FCA arthritis were significantly higher than that of the control group (p<0.05), however FCA arthritis-ozone group's level was not (p>0.05). Serum levels of IL-1 and IL-6 were not statistically significant among all groups (p>0.05). Maximum force and moment of inertia tended to increase in FCA arthritis-ozone group compare to the FCA arthritis group (p>0.05). The stiffness and toughness were similar in the all groups (p>0.05). Conclusion:To the best of our knowledge, this is the first study in which the effects of ozone on bone strength of RA were investigated. It is determined that ozone is not effective enough, but not harmful on bone strength of FCA arthritis. Amaç: Romatoid artritte (RA) periartiküler ve sistemik osteoporoza normal popülasyondan daha sık rastlanmaktadır. Bu çalışmada RA hayvan modeli olarak kabul edilen Freund's complete adjuvan (FCA) artritinde ozonun kemik dayanıklılığı üzerine olan etkisini araştırmayı amaçladık. Gereç ve Yöntem: Çalışmada 28 adet erkek Wistar sıçan kullanıldı. Sıçanlardan 14 tanesinin arka pençesine salin, kalan 14'ünün arka pençesine de ise FCA subkutan olarak enjekte edildi. İki haftanın sonunda her bir gruptan 7 sıçana, üç hafta boyunca toplam 6 kez 40 μg/kg ozon (intraperitonal olarak) uygulandı. Altıncı haftada sıçanların kan örneklerinde interlökin-1 (IL-1), IL-6 ve tümör nekroz faktör-alfa (TNF-α) düzeyleri değerlendirildi. Üç-nokta eğme testi uygulanmak üzere sağ femurları çıkarıldı. Bulgular: TNF-α düzeyi FCA artrit grubunda sağlıklı gruptan istatistiksel olarak anlamlı oranda yüksek bulunurken (p<0,05), FCA artrit-ozon grubunda bu değerler kontrol grubundan farklı bulunmadı (p>0,05). IL-1 ve IL-6 düzeylerinde ise gruplar arasında istatistiksel olarak anlamlı bir farklılık saptanmadı (p>0,05). Kemik dayanıklılığını değerlendirmede kullanılan parametreler arasında maksimum kuvvet ve atalet momenti FCA artrit-ozon grubunda FCA artrit grubuna göre yükselme eğilimi göstermesine rağmen bu değişiklikler istatistiksel olarak anlamlı bulunmadı (p>0,05). Dayanım ve sertlikte ise gruplar arasında anlamlı farklılık saptanmamıştır (p>0,05). Sonuç: Bu çalışma bildiğimiz kadarıyla RA'da kemik dayanıklılığı üzerine ozon...

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Impact of Inflammation on the Osteoblast in Rheumatic Diseases

Cited by 112 publications

References 80 publications

Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy

Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy

Comparing the diagnostic utility of sacroiliac spectral CT and MRI in axial spondyloarthritis

The Effect of Ozone on Bone Strenght in Animal Model of Rheumatoid Arthritis

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