Impact of
            <i>Helicobacter pylori</i>
            Virulence Factors and Compounds on Activation and Maturation of Human Dendritic Cells

Kranzer, Katharina; Söllner, Liane; Aigner, Michael; Lehn, Norbert; Deml, Ludwig; Rehli, Michael; Schneider-Brachert, Wulf

doi:10.1128/iai.73.7.4180-4189.2005

Cited by 63 publications

(76 citation statements)

References 67 publications

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“…Different from the LPS of other gram-negative bacteria, H. pylori LPS failed to induce the expression of those molecules (data not shown). Recently, Kranzer et al reported that the level of expression of TNF-␣ was lower in human DC cells pulsed with H. pylori WCS than in those pulsed with LB or FKB (19), which contradicts our result. The difference is probably due to the fact that they used human DCs which probably comprised a heterologous DC population rather than myeloidtype DCs selected with CD11b.…”

Section: Discussioncontrasting

confidence: 99%

“…For the pulsing of Jaws II cells with H. pylori-related antigens, ca. 100 l (WCS preparation) to 200 l (LB and FKB preparations) of H. pylori-related antigens was added to 60 to 80% confluent Jaws II cells in a 6-well culture plate and incubated at 37°C for 48 h. (8,19). The clonal proliferation of T cells exposed to LB-, FKB-, and WCS-pulsed Jaws II cells was assayed using 5-bromo-2Ј-deoxyuridine (BrdU) (15) and compared to that of T cells exposed to unpulsed Jaws II cells.…”

Section: Dcsmentioning

confidence: 99%

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Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

Otsu¹,

Gotoh²,

Yamashiro

et al. 2006

Infect Immun

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Helicobacter pylori causes persistent infection of the stomach and results in chronic gastritis and peptic ulcers. Jaws II cells, derived from mouse bone marrow, were pulsed with live or formalin-killed or whole-cell sonicates (WCS) of H. pylori. Representative cell surface molecules were expressed at substantial levels on Jaws II cells, indicating that appropriate maturation of the cells was achieved with the three H. pylori antigens without any significant differences. H. pylori WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The naïve T cells exposed to the WCS-pulsed Jaws II cells showed significant proliferation and gamma interferon (IFN-␥) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws II cells; however, no significant reductions were achieved in mice treated with Jaws II cells pulsed with other H. pylori antigens. Up-regulated production of IFN-␥ and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws II cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or H. pylori-specific antibody titers among the mice treated with Jaws II cells pulsed with the three different H. pylori antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic H. pylori infections.

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Section: Discussioncontrasting

confidence: 99%

Section: Dcsmentioning

confidence: 99%

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

Otsu¹,

Gotoh²,

Yamashiro

et al. 2006

Infect Immun

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“…In addition, we determined that the genotypes 1, 2, 3, and 4 had stimulated the secretion of IL-1β, IL-6, TNF-α, and IL-10 with different levels. Thus, our findings were similar with the previous studies (10,14,33,39,41,42).…”

Section: Discussionsupporting

confidence: 93%

“…Kranzer et al (39) reported that the cagA and vacAs2/m2 positive H. pylori strains induced the most prominent elevations of IL-1β, IL-6, IL-8, IL-12, TNF-α, and IL-10 in dendritic cells. Schmidt et al (10) reported that the cagA and vacAs2/m2 positive H. pylori strains induced low levels of IL-8 secretion in AGS cells.…”

Section: Discussionmentioning

confidence: 99%

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Caliskan

Sayi-Yazgan²,

Tokman³

et al. 2015

Turk J Gastroenterol

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Background/Aims: Helicobacter pylori (H. pylori) is a microaerophilic bacterium related with peptic ulcer and gastric cancer. Its virulence factors include cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) proteins. Cytokine release inducted by H. pylori colonization has an important role in pathogenesis of H. pylori. The severity of gastric pathologies depends on the H. pylori genotypes found in different geographical regions. We aimed to determine the relationship between different H. pylori genotypes and their effects on the cytokine release levels. Materials and Methods: ureC, cagA, vacAs1/s2, vacAm1/m2, and blood group antigen-binding adhesion protein A2 (babA2) virulence related genes were investigated in 21 H. pylori strains. Genotyping of 21 strains were made due to the presence of cagA, vacAs1/s2, vacAm1/m2, and babA2 genes. The H. pylori strains were cultured together with THP-1 and neutrophil-differentiated Human promyelocytic leukemia cells (HL-60) cells. The levels of cytokines interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor-alpha (TNF-α), and IL-10 in these cells were measured after co-culturing with H. pylori strains. Results: The following five different genotypes were detected: Genotype1: cagA and vacAs1m2; Genotype2: cagA and vacAs1m1; Genotype3: cagA, vacAs1m2, and babA2; Genotype4: vacAs2m2; and Genotype5: cagA and vacAs2m2. All these genotypes significantly induced the levels of IL-1β, IL-6, IL-8, IL 10, and TNF-α in THP-1 cells. Genotype 5 caused higher amounts of IL-1β, IL-6, TNF-α, and IL-10, whereas genotype 1 induced the highest levels of IL-8. In neutrophil-differentiated HL-60 cells, genotype 4 increased IL-6 levels and genotype 3 and 4 elevated IL-8 levels significantly. Conclusion: These results suggested that cytokine response of the host varies depending on the specific immune response of the host against different H. pylori strains.

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“…H. pylori continually stimulates gastric epithelial cells and infiltrating neutrophils, macrophages, and other lymphocytes to release proinflammatory cytokines, which enhance the inflammatory response (60,66,152). H. pylori recombinant urease is a potent stimulator of IL-12 and IFN␥ secretions from peripheral blood mononuclear cells (97), and when dendritic cells are cocultured with H. pylori, they secrete proinflammatory IL-6, IL-8, IL-12, IL-1␤, and TNF␣ (65,84) (Fig. 1).…”

Section: Chronic Inflammation and H Pylori-associated Diseasementioning

confidence: 99%

A role for altered phagosome maturation in the long-term persistence ofHelicobacter pyloriinfection

Borlace

Keep

Prodoehl

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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The vigorous host immune response that is mounted against Helicobacter pylori is unable to eliminate this pathogenic bacterium from its niche in the human gastric mucosa. This results in chronic inflammation, which can develop into gastric or duodenal ulcers in 10% of infected individuals and gastric cancer in 1% of infections. The determinants for these more severe pathologies include host (e.g., high IL-1β expression polymorphisms), bacterial [e.g., cytotoxicity-associated gene ( cag) pathogenicity island], and environmental (e.g., dietary nitrites) factors. However, it is the failure of host immune effector cells to eliminate H. pylori that underlies its persistence and the subsequent H. pylori-associated disease. Here we discuss the mechanisms used by H. pylori to survive the host immune response and, in particular, the role played by altered phagosome maturation.

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Impact of Helicobacter pylori Virulence Factors and Compounds on Activation and Maturation of Human Dendritic Cells

Cited by 63 publications

References 67 publications

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

A role for altered phagosome maturation in the long-term persistence ofHelicobacter pyloriinfection

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