Impact of foliar application of some metal nanoparticles on antioxidant system in oakleaf lettuce seedlings

Abstract

Background: Nanoparticles (NPs) serve various industrial and household purposes, and their increasing use creates an environmental hazard because of their uncontrolled release into ecosystems. An important aspect of the risk assessment of NPs is to understand their interactions with plants. The aim of this study was to examine the effect of Au (10 and 20 ppm), Ag, and Pt (20 and 40 ppm) NPs on oakleaf lettuce, with particular emphasis on plant antioxidative mechanisms. Nanoparticles were applied once on the leaves of 2-week-old lettuce seedlings, after next week laboratory analyses were performed. Results: The antioxidant potential of oakleaf lettuce seedlings sprayed with metal NPs at different concentrations was investigated. Chlorophylls, fresh and dry weight were also determined. Foliar exposure of the seedlings to metal NPs did not affect ascorbate peroxidase activity, total peroxidase activity increased after Au-NPs treatment, but decreased after applying Ag-NPs and Pt-NPs. Both concentrations of Au-NPs and Pt-NPs tested caused an increase in glutathione (GSH) content, while no NPs affected L-ascorbic acid content in the plants. Ag-NPs and Pt-NPs applied as 40 ppm solution increased total phenolics content by 17 and 15%, respectively, compared to the control. Carotenoids content increased when Ag-NPs and Au-NPs (20 and 40 ppm) and Pt-NPs (20 ppm) were applied. Plants treated with 40 ppm of Ag-NPs and Pt-NPs showed significantly higher total antioxidant capacity and higher concentration of chlorophyll a (only for Ag-NPs) than control. Pt-NPs applied as 40 ppm increased fresh weight and total dry weight of lettuce shoot. Conclusions: Results showed that the concentrations of NPs applied and various types of metal NPs had varying impact on the antioxidant status of oakleaf lettuce. Alteration of POX activity and in biosynthesis of glutathione, total phenolics, and carotenoids due to metal NPs showed that tested nanoparticles can act as stress stimuli. However, judging by the slight changes in chlorophyll concentrations and in the fresh and dry weight of the plants, and even based on the some increases in these traits after M-NPs treatment, the stress intensity was relatively low, and the plants were able to cope with its negative effects.

Antioxidant Activity of Potato Seedlings at Different Storage Temperatures

“…e values of Hunter L, a, and b were determined by wsc-s colorimeter of Shanghai Precision Instrument Co., Ltd, where L * is the brightness value, a * is the red degree, and b * is the yellow degree. C * is used to indicate the brightness of the color [13]. International Journal of Chemical Engineering e extinction value method was used.…”

Extracts from Artemisia vulgaris L. in Potato Cultivation—Preliminary Research on Biostimulating Effect

“…Chlorophyll a and chlorophyll b are the main plant dyes playing an important role in photochemical reactions of photosynthesis. In turn, carotenoids play an important role in the protection of chlorophylls against destruction under stress conditions [52,53]. Therefore, in this study the content of carotenoids in potato leaves whose plants were treated with extracts from A. vulgaris L. was evaluated.…”

Nanoparticulas de óxido de zinc y su efecto en el rendimiento y calidad de melón

“…Resultados reportan para Vigna mungo (L.), con dosis de 200 ppm de NPs ZnO los mejores resultados para germinación de semillas y crecimiento de plantas (Rajitha et al, 2020). En lechuga, reportan una mejor respuesta al aplicar 20 ppm (NPs Ag) y al aplicar el doble disminuyo el peso fresco de las hojas (Jurkow et al, 2020).…”

“…Por ejemplo, en Lycopersicon esculentum disminuyeron parámetros de crecimiento cuando se aplicaron foliarmente 200 mg L -1 de NPs ZnO (Faisan y Hayat, 2019). Las causas son diversas desde una posible toxicidad (Reed et al, 2011;Jurkow et al, 2020) o debido a factores vinculados a la forma, tamaño, periodo estacional y número de aplicaciones de las nanopartículas (Kolenčík et al, 2019).…”