Impact of Escherichia coli Outer Membrane Vesicles on Sperm Function

Folliero, Veronica; Santonastaso, Marianna; Dell’Annunziata, Federica; Franciscis, Pasquale De; Boccia, Giovanni; Colacurci, Nicola; Filippis, Anna De; Galdiero, Massimiliano; Franci, Gianluigi

doi:10.3390/pathogens11070782

Cited by 8 publications

(6 citation statements)

References 32 publications

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“…The most abundant sequences were human or unclassified in all but one sample (LRNA44), which had a top sequence assigned to the Enterobacteriaceae family. The microbes primarily within this bacterial family are Escherichia coli, which is often present in the ejaculate 31 .…”

Section: Resultsmentioning

confidence: 99%

A comprehensive analysis of spermatozoal RNA elements in idiopathic infertile males undergoing fertility treatment

Hamilton,

Russell,

Swanson

et al. 2024

Sci Rep

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Current approaches to diagnosing male infertility inadequately assess the complexity of the male gamete. Beyond the paternal haploid genome, spermatozoa also deliver coding and non-coding RNAs to the oocyte. While sperm-borne RNAs have demonstrated potential involvement in embryo development, the underlying mechanisms remain unclear. In this study, 47 sperm samples from normozoospermic males undergoing fertility treatment using donor oocytes were sequenced and analyzed to evaluate associations between sperm RNA elements (exon-sized sequences) and blastocyst progression. A total of 366 RNA elements (REs) were significantly associated with blastocyst rate (padj < 0.05), some of which were linked to genes related to critical developmental processes, including mitotic spindle formation and both ectoderm and mesoderm specification. Of note, 27 RE-associated RNAs are predicted targets of our previously reported list of developmentally significant miRNAs. Inverse RE-miRNA expression patterns were consistent with miRNA-mediated down-regulation. This study provides a comprehensive set of REs which differ by the patient’s ability to produce blastocysts. This knowledge can be leveraged to improve clinical screening of male infertility and ultimately reduce time to pregnancy.

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Section: Resultsmentioning

confidence: 99%

A comprehensive analysis of spermatozoal RNA elements in idiopathic infertile males undergoing fertility treatment

Hamilton,

Russell,

Swanson

et al. 2024

Sci Rep

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“…The presence of bacteria in semen in a signi cant number may be an indicator of male accessory gland infection or male genital tract infection [30,31]. Semen contamination may also be acquired by urinary tract organisms which have been shown to be associated with semen quality [30,32].…”

Section: Discussionmentioning

confidence: 99%

Bacteriospermia in Men Among Infertile Couples in Nepalese Population

Shrestha

Joshi

Vaidya

et al. 2023

Preprint

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Infection of male urogenital tract or male accessory glands is considered as one of the important causes of male infertility, and results in the presence of bacteria in semen affecting fertility potential of men. It is important to know the composition of bacteria in semen to understand the etiology of urogenital infections and its association with infertility. This study aims to know the rate of infection in semen of infertile men, and the association of seminal bacteria with semen parameters related to fertility potential. A cross-sectional study was carried out from June 2021 to July 2022, in which 213 semen samples were collected from the male partners of couples consulting for fertility problems in an infertility center of Nepal. All the samples were processed following WHO guidelines, 2021. Analysis of semen parameters was done immediately after the liquefaction of collected samples. Microbiological assessment was also done for identification of bacteria in semen by conventional method, which showed 25.4% of samples had bacteriospermia. S. aureus and Corynebacterium were predominant bacteria in semen. The volume of semen was significantly associated with bacteriospermia. The concentration of sperms, percentage of total sperm motility, sperms with normal forms and vitality were found to be less in semen with bacteria compared to those without bacteria, which was statistically insignificant. This study provides a baseline data on bacterial infection in semen of infertile men in Nepal.

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“…bEVs have been reported to play a role in the development and progression of several diseases including in colitis [10,11], bone loss [12,13], male infertility caused by sperm damage [14], pulmonary fibrosis [15], diabetes [16], sepsis [17], cardiovascular diseases [18] and neurological diseases [19,20] (Table 1).…”

Section: Bevs In Disease Pathogenesismentioning

confidence: 99%

“…Moreover, these UPEC OMVs induce irregular Ca 2+ oscillations with a decreased frequency in cardiomyocytes leading to cardiac injury [34], and they were also found to impair sperm motility via increasing production of intracellular reactive oxygen species and DNA fragmentation [14]. This functional diversity may be related to residual contaminants during bEV isolation and heterogeneity of bEVs generated by different biogenesis pathways or influenced by external factors (e.g.…”

Section: Gastrointestinal-associated Bacteriamentioning

confidence: 99%