Impact of environmental conditions on phospholipid fatty acid composition: implications from two contrasting estuaries

Vrana, Ivna; Novak, Tihana; Gašparović, Blaženka; Kazazić, Snježana; Čanković, Milan; Ljubešić, Zrinka; Hrustić, Enis; Mlakar, Marina; Du, Jinzhou; Zhang, Ruifeng; Zhu, Zhuoyi

doi:10.1007/s10452-020-09805-6

Cited by 6 publications

(5 citation statements)

References 67 publications

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“…6), and increased proportion of MUFA on expense of PUFA (Table 1) are small, but statistically significant. The relative invariability of PC total unsaturation and fatty acid chain lengths was found over a wide range of salinity in two estuaries studied and was explained by the maintaining the estuarine plankton cell integrity and preserving the physicochemical properties of the membranes through low variability in PC unsaturation and fatty acid chain length (Vrana Špoljarić et al 2021(Vrana Špoljarić et al , 2020. We assume that the same conclusion applies to the growth of D. tertiolecta at different salinities.…”

Section: Fatty Acid Remodeling Of Polar Lipid Species In Response To ...mentioning

confidence: 99%

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

et al. 2022

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Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater inflows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identified 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition.

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Section: Fatty Acid Remodeling Of Polar Lipid Species In Response To ...mentioning

confidence: 99%

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

et al. 2022

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“…The pigments analyzed from the samples of higher plants are reported herein, while the pigment data in the samples from the WRE and the KRE were already published (Vrana Špoljarić et al, 2021). Accordingly, the Chla data and diagnostic pigments, for both estuaries, were cited from Vrana Špoljarić et al (2021).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

“…As a karstic estuary, the nutrients and carbon in the KRE have a strong sub-ground contribution (Liu et al, 2019b). POC concentration in the KRE is very low (Vrana Špoljarić et al, 2021), largely due to the limited in situ primary production (Cauwet, 1991), except for the summer season (Cindrić et al, 2015). However, there are some anthropogenic activities in the lower reaches of the KRE, including urbanization, aquaculture (mussels farming), and shipment (Padan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Tracing Nutrients and Organic Matter Changes in Eutrophic Wenchang (China) and Oligotrophic Krka (Croatia) Estuaries: A Comparative Study

et al. 2021

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Estuaries modify the concentration and composition of riverine nutrients and organic matter (OM), which eventually determine the riverine flux effect to coasts. Nutrients, organic carbon (OC), pigments, and amino acids (AAs) from the samples collected in the eutrophic Wenchang River Estuary (WRE) in China and the oligotrophic Krka River Estuary (KRE) in Croatia were analyzed in order to have a better understanding of how estuaries regulate terrestrial materials. We found a clear increase of dissolved inorganic nitrogen (DIN) concentration and its subsequent decrease due to the removal of DIN (over 100 μM) in the WRE, whereas DIN showed minor variation lengthwise in the KRE, i.e., with the salinity changes, ranging between 1.0 and 5.8 μM. The elevated algae-derived OC, dissolved organic nitrogen, and particulate AAs nitrogen suggest that the OM assimilation may explain approximately one-third of the DIN removal in the WRE, whereas in the oligotrophic KRE, such inorganic to organic transformation is not likely to be significant. Due to the prominent estuarine nitrogen removal/assimilation process, DIN/dissolved inorganic phosphorus (DIP) ratio was as high as 425 in the upper WRE under strong riverine influence, but it declined to as low as 5.4 at the mouth of WRE, that is even lower than DIN/DIP ratio at the mouth of oligotrophic KRE (12). When compared with other rivers worldwide, the Wenchang River showed high nutrients and organic carbon yields. Given the contrasting estuarine process (e.g., DIN removal) between the KRE and the WRE, apparent high nutrient yield from eutrophic rivers should be viewed with caution in ocean studies as the final impact to coastal zone could be similar to the oligotrophic rivers like the KRE.

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“…They are an important cellular component for cell membrane function in physiological processes, energy storage, and trophic interaction in aquatic food webs. Global climate changes have a profound effect on lipids in coastal and estuarine waters and on lipid composition, which in turn affects cell integrity and physiological performance [8]. Lipid content in phytoplankton is highly dependent on environmental conditions such as CO 2 concentration, temperature, salinity, and nutrient availability [9], with CO 2 being the most important.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have shown that phytoplankton can adapt to new environmental conditions by remodeling lipid production [8,[10][11][12]. The most important group of lipids that build cell membranes are phospholipids, which define the membrane bilayers of almost all living organisms.…”

Section: Introductionmentioning

confidence: 99%

Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment

Bačinić

Vukosav

Kero

et al. 2022

JMSE

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Natural phospholipid (PL)n extract from cultured green algae Dunaliela tertiolecta was used as the main component of cell membranes for studies on the interaction with trace metal Co(II). The phospholipids of the membranes were extracted from other biological components using TiO2-µSPE cartridges selective for the phosphate group according to a completely new protocol. The interaction of Co(II) with natural and standard phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) are registered only in the presence of the additional chelating ligand 1,10-Phenathroline (Phen). 1,10-Phenathroline, as a model of humic substances in the marine environment, formed a neutral complex with Co(II) by the substitution of water molecules of central metal ions. The interaction of hydrophobic mixed-ligand complexes with phospholipids was enabled by the substitution of the remaining water molecules in the coordination shell of Co(II), which was registered by voltammetric measurements. The Co(II)-Phen-PL complex is reduced from the adsorbed state at −1.65 V by the transfer of two electrons, followed by its irreversible dissociation and desorption, indicating an EC mechanism. The interaction between the mixed-ligand complexes Co(II)-Phen-lipids was confirmed by atomic force microscopy (AFM). AFM images of PL, PL with Phen mixture and PL, Phen with Co(II) showed different 3D structures on the mica surface, indicating changes caused by the interaction between cobalt(II), 1,10-Phenanthroline and phospholipids.

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Impact of environmental conditions on phospholipid fatty acid composition: implications from two contrasting estuaries

Cited by 6 publications

References 67 publications

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

Tracing Nutrients and Organic Matter Changes in Eutrophic Wenchang (China) and Oligotrophic Krka (Croatia) Estuaries: A Comparative Study

Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment

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