Impact of Enhanced Mobilization of Bone Marrow Derived Cells to Site of Injury

Abstract

Local SDF-1 and/or systemic G-CSF can effectively increase BMDC homing to sites of traumatic injury in an additive way and improve wound healing. This process appears to be mediated predominantly through MSC. Additional investigations are needed to identify the optimal adjuncts to improve wound healing following severe traumatic injury.

The Effect of Granulocyte-colony Stimulating Factor on Rotator Cuff Healing After Injury and Repair

“…Lastly, we did not perform an analysis of the mobilization effect of subcutaneous G-CSF administration or a phenotypic study of the cells that were recruited to the surgical site. The effect of G-CSF administration on stem cell mobilization is well established in murine and other models [11,22,28,35,37,46].…”

“…staining was performed to identify the infiltrating cells, previous studies using stem cell mobilization agents in the setting of cerebrovascular ischemia [41], acute myocardial infarction [35], and lung contusion [22] demonstrated similar increases in cellularity and have determined that cells mobilized from marrow cavities indeed transmigrate into injured tissues.…”

“…Rats were euthanized by CO 2 asphyxiation at 12 and 19 postoperative days. These time points were selected to assess early differences between treatment groups and represent similar time points for other studies that have used G-CSF in murine models of lung contusion [22] and myocardial infarction [35]. Recent studies also indicate early systemic effects associated with G-CSF therapy [37,41].…”

“…Animals in the G-CSF group received subcutaneous injections of 100 lg/kg G-CSF (Filgastrim; Amgen, Thousand Oaks, CA, USA) each day for 5 postoperative days. This dose mirrors clinical use of G-CSF and is commonly used within murine models to promote the mobilization of stem cells into peripheral blood [22]. The control animals received normal saline injections of equivalent volume.…”

Adult stem cells: potential implications for perioperative medicine

“…4 Controversy exists as to the physiological role of these cells under native in vivo conditions; however, it has been suggested that MSCs may be perivascular cells (''pericytes'') that support vascular homeostasis and tissue repair. In preclinical studies, perivascular MSCs appear to be mobilized into the circulation under inflammatory conditions, such as traumatic lung injury 5 or hypoxia. 6 A similar mobilization of MSCs into the circulation has also been observed clinically in elderly patients following traumatic hip fracture.…”