Impact of different platelet-rich fibrin (PRF) procurement methods on the platelet count, antimicrobial efficacy, and fibrin network pattern in different age groups: an in vitro study

Mamajiwala, Alefiya; Sethi, Kunal Sunder; Raut, Chetan Purushottam; Karde, Prerna Ashok; Mangle, Nikita M.

doi:10.1007/s00784-019-03022-8

Cited by 17 publications

(15 citation statements)

References 48 publications

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“…Additionally, this natural scaffold is easily prepared in the veterinary clinic, having minimal contamination risks when prepared with aseptic technique, due to its minimal manipulation. PRFs have been also advocated to have bactericidal proprieties [43].…”

Section: Presenting the Hemoderivatives With Therapeutic Interestmentioning

confidence: 99%

Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Soares

Babo

Reis

et al. 2021

Trends in Biotechnology

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Section: Presenting the Hemoderivatives With Therapeutic Interestmentioning

confidence: 99%

Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Soares

Babo

Reis

et al. 2021

Trends in Biotechnology

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“…also used Leishman stain to estimate the platelets and showed more than 90% inclusion of platelets in PRF matrix. [ 14 ] They proposed that increase in centrifugation speed and time leads to reduction of platelet inclusion in matrix, contrary to the study by Ghanaati et al . who proposed reduction in speed but increase in time of centrifugation.…”

Section: Discussionmentioning

confidence: 89%

Evaluation of histological and pH changes in platelet-rich fibrin and platelet-rich fibrin matrix: A In vitro study

et al. 2019

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Background: The autologous platelet concentrates (PCs), such as platelet-rich fibrin (PRF) and platelet-rich fibrin matrix (PRFM), are processed through different centrifugation protocols, which can affect their biological properties and in turn influence treatment outcome. The pH value can influence the process of wound healing directly or indirectly. Hence, a study was conducted to evaluate fibrin network pattern, initial pH of obtained matrix, and its changes during degradation matrix and to determine platelet and leukocyte count in PRF and PRFM. Materials and Methods: Blood from the volunteers was collected in blood vacutainers for processing PRF and PRFM. It was centrifuged as per the standard protocol. Serum from PRF and PRFM was subjected for analysis of platelet and leucocyte concentration using Hemo-Analyzer. The pH of PRF and PRFM were evaluated over 5 days using a pH meter. PRF and PRFM were subjected to histological and scanning electron microscopic analysis. Results: There was no significant variation in the platelet and leukocyte count between PRF and PRFM. A steady rise in the level of pH with respect to PRFM was observed, whereas there was a decrease in the pH levels in PRF. Fibrin network was denser in PRF compared to PRFM. Conclusion: The PCs undergo variation in pH upon degradation. Formation of fibrin matrix is influenced by the method of preparation. Fibrin pattern is crucial to facilitate adhesion of cells and transport of nutrients to enable proliferation and differentiation of mesenchymal cells and better wound healing.

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“…As a possible further explanation of the retrieved results, one must reconsider the inter-individual differences between PRF as an autologous platelet concentrate. Besides different procuring protocols, it was shown that membrane size and release of the proangiogenic growth factors of the respective autologous platelet concentrate, such as PRP or PRF, highly depend on age and gender [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Non-Interventional Prospective Observational Study of Platelet Rich Fibrin as a Therapy Adjunctive in Patients with Medication-Related Osteonecrosis of the Jaw

Blatt

Krüger

Kämmerer

et al. 2022

JCM

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Background: Medication-related osteonecrosis (MRONJ) of the jaw is a severe and feared side effect of antiresorptive therapy in the oncological setting. With growing evidence that impaired angiogenesis may represent a key factor in pathogenesis, the aim of this study was to evaluate an autologous platelet concentrate as a possible additive in surgical therapy to optimize vascularization and, subsequently, resolution rates. Material and Methods: A non-interventional, prospective, multicenter study was conducted, and all patients with stage I-III MRONJ, undergoing antiresorptive therapy for an oncological indication, were included. The necrosis was treated surgically without (study arm A) or with (arm B) the addition of an autologous platelet concentrate (platelet-rich fibrin, PRF). Results: After 5, 14, and 42 days postoperative, wound healing (primary outcome: mucosal integrity) as well as downstaging, pain perception, and oral health-related quality of life (secondary outcome) were assessed via clinical evaluation. Among the 52 patients included, primarily with MRONJ stage I and II, the use of PRF as an additive in surgical therapy did not display a significant advantage for wound healing (p = 0.302), downstaging (p = 0.9), pain reduction (p = 0.169), or quality of life (p = 0.9). Summary: In conclusion, PRF as an adjunct did not significantly optimize wound healing. Further, no significant changes in terms of downstaging, pain sensation, and oral health-related quality of life were found.

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Impact of different platelet-rich fibrin (PRF) procurement methods on the platelet count, antimicrobial efficacy, and fibrin network pattern in different age groups: an in vitro study

Cited by 17 publications

References 48 publications

Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Evaluation of histological and pH changes in platelet-rich fibrin and platelet-rich fibrin matrix: A In vitro study

Non-Interventional Prospective Observational Study of Platelet Rich Fibrin as a Therapy Adjunctive in Patients with Medication-Related Osteonecrosis of the Jaw

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