Impact of Different Phytohormones on Morphology, Yield and Cannabinoid Content of Cannabis sativa L.

Burgel, Lisa; Hartung, Jens; Schibano, Daniele; Graeff‐Hönninger, Simone

doi:10.3390/plants9060725

Cited by 20 publications

(24 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To ensure the needed high quality standards for medical and pharmaceutical products, it is necessary to scientifically develop and evaluate cultivation methods. Recently, there has been an increased focus on arbuscular mycorrhiza [ 7 ], cultivation substrates [ 8 , 9 ], plant density [ 10 ], phytohormones [ 11 ] and the effect of fungal pathogens [ 12 ] to further develop cultivation systems.…”

Section: Introductionmentioning

confidence: 99%

Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Reichel

Munz

Hartung

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

Cannabis is one of the oldest cultivated plants, but plant breeding and cultivation are restricted by country specific regulations. Plant growth, morphology and metabolism can be manipulated by changing light quality and intensity. Three morphologically different strains were grown under three different light spectra with three real light repetitions. Light dispersion was included into the statistical evaluation. The light spectra considered had an influence on the morphology of the plant, especially the height. Here, the shade avoidance induced by the lower R:FR ratio under the ceramic metal halide lamp (CHD) was of particular interest. The sugar leaves seemed to be of elementary importance in the last growth phase for yield composition. Furthermore, the last four weeks of flowering were crucial to influence the yield composition of Cannabis sativa L. through light spectra. The dry flower yield was significantly higher under both LED treatments compared to the conventional CHD light source. Our results indicate that the plant morphology can be artificially manipulated by the choice of light treatment to create shorter plants with more lateral branches which seem to be beneficial for yield development. Furthermore, the choice of cultivar has to be taken into account when interpreting results of light studies, as Cannabis sativa L. subspecies and thus bred strains highly differ in their phenotypic characteristics.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Reichel

Munz

Hartung

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cannabinoid extraction was performed according to Burgel et al [28]. Quantitative analysis of cannabinoids, particularly cannabidiol (CBD), cannabidiolic acid (CBDA), ∆ 9 -tetrahydrocannabinol (THC) and tetrahydrocannabinolic acid (THCA), was performed, according to the HPLC methods of Lehmann and Brenneisen [29] with slight modifications after Burgel et al [30].…”

Section: Extraction and Quantification Of Cannabinoids By Hplc Analysismentioning

confidence: 99%

Impact of Different Growing Substrates on Growth, Yield and Cannabinoid Content of Two Cannabis sativa L. Genotypes in a Pot Culture

2020

Self Cite

View full text Add to dashboard Cite

The impacts of different growing substrate compositions, consisting of peat (PM), peat substituted with 30% green fibre (G30) and coco coir fibre (CC) growth media, were investigated in regard to the plant height, biomass and floral yield, biomass nitrogen (N) content, root growth, and cannabidiol content (CBD/A) of two phytocannabinoid-rich cannabis genotypes in an indoor pot cultivation system. Genotypes and substrate treatment combinations were randomly allocated to 36 plants according to a Latin square design. The results showed a higher total plant height for PM (39.96 cm), followed by G30 (35.28 cm), and the lowest in CC (31.54 cm). The N content of leaves indicated the highest values for plants grown in G30 (52.24 g kg DW−1), followed by PM (46.75 g kg DW−1) and a significantly lower content for CC (37.00 g kg DW−1). Root length density (RLD) increased by 40% (PM) and 50% (G30), compared to CC treatments, with no significant differences in root dry weight. Both genotypes, Kanada (KAN) and 0.2x, reacted in a genotype-specific manner. KAN indicated a reduced floral yield of plants grown in G30 (4.94 g plant−1) and CC (3.84 g plant−1) compared to PM (8.56 g plant−1). 0.2x indicated stable high floral yields of 9.19 g plant−1 (G30) to 7.90 g plant−1 (CC). Leaf DW increased in PM (5.78 g plant−1) and G30 (5.66 g plant−1) compared to CC (3.30 g plant−1), while CBD/A content remained constant. Due to a higher biomass yield, the CBD/A yield of flowers (549.66 mg plant−1) and leaves (224.16 mg plant−1) revealed 0.2x as an interesting genotype for indoor pot cultivation in a peat-based substrate substituted with 30% green fibres. Overall, the demand for organic green fibres to partly replace fractionated peat showed a genotype-specific option for a homogeneous plant development, with comparable high biomass yields and stable cannabinoid contents compared to a peat containing standard substrate.

show abstract

“…Previous studies in other plant species revealed that different tissues and different environmental conditions would require unique reference genes to accurately interpret the expression of speci c genes 35,36 . Eleven different conditions including osmotic stresses, heavy metal stresses, plant hormone stimulus, and UV light application were reported to affect the cannabinoid synthesis 37,38,39,40 . Results obtained in this study from four programs: geNorm, NormFinder, BestKeeper, and RefFinder were not consistent, particularly BestKeeper which was much more distinct from the other software methods.…”

Section: Discussionmentioning

confidence: 99%

Selection and validation of reference genes for normalization of qRT-PCR data to study secondary metabolite related genes in industrial hemp

Deguchi¹,

Potlakayala²,

Spuhler³

et al. 2021

Preprint

View full text Add to dashboard Cite

Industrial hemp (Cannabis sativa L.) is a dioecious crop widely known for its production of phytocannabinoids, flavonoids, and terpenes. In the past two years since its legalization, there has been significant interest in researching this important crop for pharmaceutical applications. Although many scientific reports have demonstrated gene expression analysis of hemp through OMICs approaches, accurate validation of omics data cannot be performed because of lack of reliable reference genes for normalization of qRT-PCR data. The differential gene expression patterns of 13 candidate reference genes under osmotic, heavy metal, hormonal, and UV stress were evaluated through four software packages: geNorm, NormFinder, BestKeeper, and RefFinder. The EF-1a ranked as the most stable reference gene across all stresses, TUB was the most stable under osmotic stress, and TATA was the most stable under both heavy metal and hormonal stress. The expression profiles of two cannabinoid pathway genes, AAE1 and THCAS, using the two most stable and single least stable reference genes confirmed that two most stables genes were apt for normalization of gene expression data. This work will contribute to the future studies on the expression analysis of hemp genes regulating the synthesis, transport and accumulation of secondary metabolites.

show abstract

Impact of Different Phytohormones on Morphology, Yield and Cannabinoid Content of Cannabis sativa L.

Cited by 20 publications

References 47 publications

Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Impact of Different Growing Substrates on Growth, Yield and Cannabinoid Content of Two Cannabis sativa L. Genotypes in a Pot Culture

Selection and validation of reference genes for normalization of qRT-PCR data to study secondary metabolite related genes in industrial hemp

Contact Info

Product

Resources

About