Immunostimulatory potential of β-lactoglobulin preparations

Brix, Susanne; Bovetto, Lionel; Fritsché, Rodolphe; Barkholt, Vibeke; Frøkiær, Hanne

doi:10.1016/j.jaci.2003.08.047

Cited by 43 publications

(43 citation statements)

References 41 publications

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“…We found a marked production of IL-10 in PBMCs on stimulation with b-LG in all four groups analyzed, and nonallergic healthy children displayed the highest IL-10 level. Although we cannot exclude that the high IL-10 production to b-LG observed in all four analyzed groups could be partially because of an LPS-like activity of this allergen (28,29), it remains interesting that healthy children showed the highest number of IL-10-producing cells and that IL-10 was able to down-regulate the PBMCs' proliferative response to b-LG stimulation.…”

Section: Discussionmentioning

confidence: 87%

Peripheral Blood Immune Response Elicited by Beta-Lactoglobulin in Childhood Cow's Milk Allergy

et al. 2011

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Several studies analyzing the immune responses in patients with cow's milk allergy (CMA) have used T-cell lines or T-cell clones that require prolonged in vitro cell culturing and may result in a switched cell phenotype and function. We investigated immune responses to beta-lactoglobulin (b-LG) in peripheral blood mononuclear cells after a short in vitro antigen stimulation in children with acute CMA (both IgE-mediated and non-IgE-mediated forms) and in those who outgrew an IgE-mediated CMA. Healthy controls were also investigated. Peripheral blood mononuclear cells were assayed for IL-13, IFN-␥, IL-4, and IL-10. Although b-LG induced a cytokine production and/or cell proliferation almost in all children, included healthy controls, differences were observed among the four groups. Children with IgE-mediated CMA had a marked Th2-response, with high IL-13 production and proliferation, but low IFN-␥; by contrast, children with non-IgE-mediated CMA produced no, or very low, IL-13 and cell proliferation. Children, who outgrew CMA, showed a shift to a Th1-response, with reduced IL-13 and increased IFN-␥. IL-10-responses were high in all groups, with the highest level in healthy children; by contrast, IL-4 was undetectable in all children. This study highlights the use of shortly stimulated peripheral blood cells to investigate the food-induced immune responses. (Pediatr Res 70: 549-554, 2011)

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Section: Discussionmentioning

confidence: 87%

Peripheral Blood Immune Response Elicited by Beta-Lactoglobulin in Childhood Cow's Milk Allergy

et al. 2011

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“…Mercier et al (2004) found whey protein from isolates to significantly induce proliferation of splenic lymphocytes without mitogen stimulation. Brix et al (2003) reported that a commercial β-lactoglobulin preparation significantly increased proliferation of spleen lymph nodes and stimulated the cellular glutathione concentration in spleen cell cultures.…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary whey protein concentrate level on performance, selected intestinal tract and blood parameters, and thiobarbituric acid reactive substances in the liver and breast meat of broiler chickens

Szczurek¹,

Szymczyk²,

Arczewska-Włosek³

et al. 2013

J. Anim. Feed Sci.

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ABSTRACT. The aim of this study was to investigate the effect of two levels of whey protein concentrate (WPC, 80.35% protein, 5.86% lactose) added to diets and duration of these treatments on growth and slaughter value, haematological parameters involved in immune processes, oxidative status of liver and breast muscle, and selected indices of gut function of broilers. A total of 560 Ross 308 broilers were randomly allocated into 7 groups with 10 replicates of 8 chickens per replicate. The experiment lasted 42 days and was divided into 3 successive feeding phases. The control group was fed basal diets consisted of maize, wheat and soyabean meal (C group). The other 6 groups received, for 7, 21 and 42 days basal diets with 8 or 32 g · kg -1 WPC added at the expense of soyabean meal (WL and WH groups, respectively). On day 42 group WH had a higher lymphocyte percentage (P < 0.05) compared to the control. The long-term (42 days) feeding with higher level of WPC significantly decreased the heterophile/lymphocyte ratio (P < 0.05 vs C). The number of erythrocytes on day 42 and the whole blood glucose on day 21 were significantly increased in WH birds than in controls. The concentration of serum total protein on both WPC levels was greater (P < 0.05) on day 21 compared with the C group. The reduction (P < 0.05) in liver thiobarbituric acid reactive substances (TBARS) and a tendency towards lower TBARS level in breast meat were observed in birds WH when fed for 42 days. Only higher dietary WPC amount provided for 42 days reduced the caecal and small intestinal pH values (P < 0.05 vs C). The ileal digestibility of crude protein was higher (P < 0.01) in birds fed with 32 g WPC during the first 21 days of life, compared to the C and WL treatments. The factorial ANOVA showed that on days 7, 21 and 42 the final body weight gain (BGW) and feed conversion ratio in broilers WL and WH were significantly better than in the C ones. Assessment of orthogonal contrasts revealed significantly higher (P < 0.05) final BWG in the WH broilers, compared to the WL dietary treatment; such effect was not observed in earlier feeding stages. Generally, carcass yield on both WPC dietary levels, and breast muscle percentage on WH treatment, were higher (P < 0.01 and P < 0.05, respectively) compare to the C group. In conclusion, WPC added to a conventional diets for growing chickens exerts a growth-promoting action and at a dose of 32 g · kg -1 may induce desirable changes in bird's health and the intestinal tract metabolism. W. Szczurek et al. 343

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“…The range of LPS contamination reported for different allergen preparations is quite broad [30]. Similarly, commercially available protein preparations, such as OVA [6] and β-lactoglobulin may contain different amounts of LPS, depending on the manufacturer and the way of preparation [31]. Compared to the varying LPS levels reported in different studies, the endotoxin content of our rFel d 1 preparation and the amount of LPS added to cell cultures in this study can be considered as low doses.…”

Section: Discussionmentioning

confidence: 99%

Low Levels of Endotoxin Enhance Allergen-Stimulated Proliferation and Reduce the Threshold for Activation in Human Peripheral Blood Cells

Veličković

Thunberg

Polović

et al. 2007

Int Arch Allergy Immunol

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Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS /mg protein). PBMCs prepared from 15 subjects having IgE to cat (>0.7 kU_A/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 µg/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFNγ, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-positive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 µg/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFNγ in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro.

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Immunostimulatory potential of β-lactoglobulin preparations

Cited by 43 publications

References 41 publications

Peripheral Blood Immune Response Elicited by Beta-Lactoglobulin in Childhood Cow's Milk Allergy

Peripheral Blood Immune Response Elicited by Beta-Lactoglobulin in Childhood Cow's Milk Allergy

The effects of dietary whey protein concentrate level on performance, selected intestinal tract and blood parameters, and thiobarbituric acid reactive substances in the liver and breast meat of broiler chickens

Low Levels of Endotoxin Enhance Allergen-Stimulated Proliferation and Reduce the Threshold for Activation in Human Peripheral Blood Cells

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