Immunophenotypic changes induced on human HL60 leukaemia cells by 1α,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate

White, Sarah L.; Belov, Larissa; Barber, Nicole; Hodgkin, Philip D.; Christopherson, Richard I.

doi:10.1016/j.leukres.2005.02.012

Cited by 42 publications

(52 citation statements)

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“…Amplified DNA was subjected to electrophoresis in 2% agarose, stained with SYBR Green I (Molecular Probes Ltd., Eugene, OR, USA), and imaged and calculated using a FluorImager (Molecular Dynamics Tokyo Ltd., Tokyo, Japan) as For RT-PCR GAPDH 5'-AAGGTCATCCCTGAGCTGAA-3' 5'-CCCCTCTTCAAGGGGTCTAC-3' 497 Exportin 5 5'-TAAACCGTGCACGAATGAGC-3' 5'-TGCAGGGGTTACGGAAGATT-3' 152 Exportin 6 5'-GTGTGCCTTTCACTGAGCAA-3' 5'-CGGAAAAGGAGCTCAAACAG-3' 273 Exportin 7 5'-TGGGCGTTGTGAATATGAGA-3' 5'-ACTTCTGAGAGTCGGCGGTA-3' 392 For real-time PCR GAPDH 5'-CTAGGGTGTGAACCATGAGA-3' 5'-TGTGGTCATGAGTCCTTCCA-3' 231 Exportin 5 5'-TAAACCGTGCACGAATGAGC-3' 5'-TGCAGGGGTTACGGAAGATT-3' 152 Exportin 6 5'-GTGTGCCTTTCACTGAGCAA-3' 5'-GATACACTTGCTCCATGCACA-3' 212 Exportin 7 5'-TGGGCGTTGTGAATATGAGA-3' 5'-CACCGATCACTGCTCCAATA-3'20, 27), S-100 calcium-binding proteins S-100A8, A9, and A4 (9), cytokines interleukin 8, interleukin 1β, and tumor necrosis factor (14), lysosomal enzyme lysozyme (24), and cell cycle-related proteins cyclin D2 (3,25) and p21/Cip 1 (17,25). These findings were consistent with changes observed previously at gene and/or protein levels to indicate differentiation toward a monocyte/macrophage lineage (8,9,14,17,20,24,25,27). Genes down-regulated were those for proteins related to protein synthesis (isoleucine-tRNA synthetase, phenylananyl-tRNA synthetase beta subunit, cytidine 5'-triphosphate synthase, and arginine/serine-rich 7.3 kDa splicing factor), the nuclear transport protein exportin-tRNA, and the cell cyclerelated protein cyclin D2.…”

Section: Rt-pcrsupporting

confidence: 92%

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DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells

et al. 2006

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Using a DNA microarray, we analyzed about 16,600 genes for changes in expression associated with the differentiation of human promyelocytic leukemia HL-60 cells induced by 1α,25-dihydroxyvitamin D 3 (DVD). Many of the up-regulated genes could be correlated to differentiation-associated changes toward a monocyte/macrophage lineage, and many down-regulated genes could be correlated to repressed cell growth. The present study revealed the down-regulated gene expression of importins and exportins 1, 5, 7, and exportin-tRNA. Thus, the present results confirmed our previous findings of down-regulation of exportin 1 and exportin-tRNA by DVD. Gene expression of exportin 6 is suggested to be regulated differently from that of exportins 1, 5, 7, and exportin-tRNA. The down-regulation of nuclear transport factors may be deeply associated with the differentiation of HL-60 cells induced by DVD.The active form of vitamin D 3 , 1α, 25-dihydroxyvitamin D 3 (DVD), results from sequential hydroxylation of vitamin D 3 in the liver and kidney and is responsible for calcium homeostasis in the body. Vitamin D 3 metabolites have an important role in the proliferation of hematopoietic cells, lymphocytes, and keratinocytes, as well as other cell types (17). DVD is a potent inducer of cell differentiation in human leukemia HL-60 cells (6,10,18,24). The HL-60 cell line, derived from a single individual with acute promyelocytic leukemia, provides a unique in vitro system for studying the cellular and molecular events involved in the proliferation and differentiation of normal and leukemic cells of the granulocyte/monocyte/macrophage lineage (5).In spite of extensive studies (20,25), it remains unclear how DVD exhibits biological activities including induction of differentiation, growth inhibition, and apoptosis. In our previous study, we examined changes in gene expression during the DVD-induced differentiation of HL-60 cells employing a DNA microarray technique and found the gene expression of the β subunit of eukaryotic translation initiation factor 2 to be suppressed, suggesting a linkage to differentiation-associated growth inhibition of these cells (7,22). Since the microarray used covered only 425 genes, the study provided us with limited information.In the present study, we analyzed about 16,600 genes for changes in expression associated with the DVD-induced differentiation of HL-60 cells. Reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR were used to examine the expression of some selected genes of our interest to evaluate the validity of cDNA microarray analysis.

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Section: Rt-pcrsupporting

confidence: 92%

“…Blue spots represent expressed genes with a significant fluorescence signal to be analyzed in the present study. HL-60 cells toward a monocyte/macrophage lineage (16,27).…”

Section: Changes In Importin Expressionmentioning

confidence: 99%

DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells

et al. 2006

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“…The surface profiles of the monocyte-and macrophage-like cells induced by 1,25D 3 and TPA, respectively, are not identical to those obtained from normal monocytes and macrophages [17]. However, Fig.…”

Section: -O-tetradecanoyl Phorbol-13-acetatementioning

confidence: 71%

“…The human cell line HL-60, is derived from a case of the M2 subtype of AML and is used as a model for drug-induced differentiation of promyelocytic leukaemia [16]. Drugs such as ATRA, 12-O-tetradecanoyl phorbol-13-acetate (TPA) or 1-a,25-dihydroxyvitamin D 3 (1,25D 3 ) induce HL-60 cells to differentiate into mature myeloid cells with loss of proliferative capacity [17]. ATRA induces major changes in membrane proteins, some of which are adhesion molecules that interact with neighbouring cells.…”

Section: All-trans Retinoic Acidmentioning

confidence: 99%

Profiling CD antigens on leukaemias with an antibody microarray

Barber¹,

Gez²,

Belov³

et al. 2009

FEBS Letters

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Edited by Miguel De la RosaKeywords: Leukaemia Lymphoma Cluster of differentiation antigen Microarray a b s t r a c t Cluster of differentiation (CD) antigens are defined when a surface molecule found on some members of a standard panel of human cells reacts with at least one novel antibody, and there is good accompanying molecular data. Monoclonal antibodies to surface CD antigens on leukocytes have been used for flow cytometry, and more recently to construct microarrays that capture live cells. These DotScan TM microarrays enable the rapid and highly parallel characterization of repertoires of CD antigens whose expression patterns may be correlated with discrete leukaemia subtypes, or used to define biomarker 'signatures' for non-hematological diseases. DotScan TM with fluorescence multiplexing enables profiling of CD antigens for minor subsets of cells, such as colorectal cancer cells and tumour-infiltrating lymphocytes from a surgical sample.

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“…8) On the other hand, 1,25-VD 3 and TPA have been established to induce differentiation of HL-60 cells to mature monocytes. 9) DMSO is another typical cell differentiation inducer.…”

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confidence: 99%

Enhancement of Chemically-Induced HL-60 Cell Differentiation by 3,3'-Diindolylmethane Derivatives

et al. 2009

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3,3Ј-Diindolylmethane (DIM, 1) is a major metabolite (spontaneously formed product in intragastric acid condition) of indole-3-carbinol (I3C, 2) which is a phytochemical expressed in brassica vegetables, including broccoli, brussels sprouts, kales and the cabbages, and has been thought to be associated with the anticancer/cancer chemopreventive activities of vegetable consumption.1) DIM (1) controls proliferation of various tumor cells, including cells of breast cancer, prostate cancer, ovary cancer, lung cancer and pancreatic cancer, by inducing G 1 /S arrest of the cell cycle and apoptosis.2) In addition, DIM (1) modulates metabolism of estrogen and testosterone, 3,4) which act as indigenous tumor promoters for several hormone-dependent cancers. DIM (1) is also reported to be a weak agonist for the arylhydrocarbon receptor (AhR) and blocks the effects of estrogens via inhibition of AhR-estrogen receptor cross talk.5) In addition, DIM (1) possesses other various activities, including immunomodulatory effect.6) All the experimental studies suggest that DIM (1) has incredible potential both for prevention and for treatment of cancer.Although the molecular basis of cancer chemopreventive and cancer chemotherapic effects of DIM (1) is unclear, effects of DIM (1) on cell differentiation would be one of candidate mechanisms underlying the biological activities elicited by DIM (1). In fact, Kim et al. recently reported that indirubin, another AhR agonist with a structure related to that of DIM (1), and its derivatives show enhancing effect on 1,25-dihydroxyvitamin D 3 (1,25-VD 3 )-and all-trans retinoic acid (ATRA)-induced cell differentiation of HL-60 leukemia cells. 7)Under such circumstances, we investigated the enhancing effect on chemically-induced HL-60 cell differentiation of DIM (1) and its derivatives in combination with various cell differentiation inducers, including ATRA, tamibarotene (Am80, 3), 1,25-VD 3 , 12-O-tetradecanoyl phorbol-13-acetate (TPA) and dimethyl sulfoxide (DMSO). All of these chemicals are known to induce terminal differentiation in leukemic cell lines, including HL-60 and U937 cells. ATRA and Am80 are therapeutic agents for acute promyelocytic leukemia (APL) and induce differentiation of HL-60 cells to mature granulocytes.8) On the other hand, 1,25-VD 3 and TPA have been established to induce differentiation of HL-60 cells to mature monocytes. 9) DMSO is another typical cell differentiation inducer. 10) Results and DiscussionChemistry DIM (1), its derivatives (4-17), and isomers of DIM [2,2Ј-DIM (22) and 2,3Ј-DIM (25) Bunkyo-ku, Tokyo 113-0032, Japan. Received January 8, 2009; accepted February 19, 2009; published online February 24, 2009 3,3-Diindolylmethane (DIM, 1) and its derivatives have been prepared, and their enhancing effects on chemically-induced HL-60 cell differentiation were analyzed. Among the prepared compounds, IndDIM (12) showed the most potent enhancing effect on HL-60 cell differentiation induced by chemicals, including retinoids, 1,25-dihydroxyvitamin D 3 , 12-O-...

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Immunophenotypic changes induced on human HL60 leukaemia cells by 1α,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate

Cited by 42 publications

References 50 publications

DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells

DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells

Profiling CD antigens on leukaemias with an antibody microarray

Enhancement of Chemically-Induced HL-60 Cell Differentiation by 3,3'-Diindolylmethane Derivatives

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