Archives of Dermatology
 1979
DOI: 10.1001/archderm.115.4.433
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Immunoperoxidase localization of Sporothrix schenckii and Cryptococcus neoformans. Staining of tissue sections fixed in 4% formaldehyde solution and embedded in paraffin

B. Russell1

Abstract: An indirect immunoperoxidase staining method has been successfully applied to 4% formaldehyde solution tissue sections fixed in and embedded in paraffin for the localization of Sporothrix schenckil and Cryptococcus neoformans without prior trypsinization of tissue sections. A comparison of this method with an analogous immunofluorescence staining technique has been made.

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“…When sections of these fungi were stained with the PAS reagent, This technique can be used to identify certain fungi in smears and in formalin-fixed, paraffin-embedded tissue sections. 47 -49 This technique, however, up to now, has only limited diagnostic use. 50 .…”
Section: Autofluorescencementioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…When sections of these fungi were stained with the PAS reagent, This technique can be used to identify certain fungi in smears and in formalin-fixed, paraffin-embedded tissue sections. 47 -49 This technique, however, up to now, has only limited diagnostic use. 50 .…”
Section: Autofluorescencementioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…A definitive diagnosis relies on culture from a swab, lesion aspiration, or tissue biopsy; the yeast may be detected in tissue by immuno‐peroxidase techniques 5 . The tissue sample should be inoculated onto Sabouraud or blood agar and incubated at room temperature.…”
Section: Diagnosismentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
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