Immunoperoxidase localization of Sporothrix schenckii and Cryptococcus neoformans. Staining of tissue sections fixed in 4% formaldehyde solution and embedded in paraffin

Russell, B.

doi:10.1001/archderm.115.4.433

Cited by 9 publications

(2 citation statements)

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“…When sections of these fungi were stained with the PAS reagent, This technique can be used to identify certain fungi in smears and in formalin-fixed, paraffin-embedded tissue sections. 47 -49 This technique, however, up to now, has only limited diagnostic use. 50 .…”

Section: Autofluorescencementioning

confidence: 99%

Fungal Infections

Haque

McGinnis

2008

Dail and Hammar’s Pulmonary Pathology

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The incidence of invasive fungal infections has increased dramatically over the past two decades, mostly due to an increase in the number of immunocompromised patients. l -4 Patients who undergo chemotherapy for a variety of diseases, patients with organ transplants, and patients with the acquired immune deficiency syndrome have contributed most to the increase in fungal infections. s The actual incidence of invasive fungal infections in transplant patients ranges from 15% to 25% in bone marrow transplant recipients to 5% to 42% in solid organ transplant recipients. 6 ,7 The most frequently encountered are Aspergillus species, followed by Cryptococcus and Candida species. Fungal infections are also associated with a higher mortality than either bacterial or viral infections in these patient popUlations. This is because of the limited number of available therapies, dose-limiting toxicities of the antifungal drugs, fewer symptoms due to lack of inflammatory response, and the lack of sensitive tests to aid in the diagnosis of invasive fungal infections. I A study of patients with fungal infections admitted to a university-affiliated hospital indicated that communityacquired infections are becoming a serious problem; 67% of the 140 patients had community-acquired fungal pneumonia. 8 There is also an increase in nosocomial fungal infections.9Fungi are eukaryotic, unicellular to multicellular organisms that have chitinous cell walls, and reproduce asexually, sexually, or both ways. Fungal cells are larger and genomically more complex than bacteria. Their cell wall contains polysaccharides, proteins, and sugars, and their antigens are rich in complex polysaccharides and glycoproteins. Plasma membranes of fungi contain ergosterol, which is the primary target for antifungals such as amphotericin B. Although there are more than 1.3 million fungal species in the environment, only about 150 are known to be pathogenic to humans. For detailed taxonomy of the fungi, several texts are available. W-12 The virulence factors of fungi resemble those of bacteria, such as possession of a capsule, adhesion molecules, toxins, free radicals, etc.

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Section: Autofluorescencementioning

confidence: 99%

Fungal Infections

Haque

McGinnis

2008

Dail and Hammar’s Pulmonary Pathology

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“…A definitive diagnosis relies on culture from a swab, lesion aspiration, or tissue biopsy; the yeast may be detected in tissue by immuno‐peroxidase techniques 5 . The tissue sample should be inoculated onto Sabouraud or blood agar and incubated at room temperature.…”

Section: Diagnosismentioning

confidence: 99%

A Rose by Any Other Name

Engle

Desir

Bernstein

2007

Skinmed

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A 40-year-old man visited Haiti in the winter. His visit was uneventful, and he went swimming in the ocean. A week after his return he developed a small "pimple" on his right fifth finger. This condition progressed for several weeks, with new lesions developing over the extensor surface of his forearm and in the antecubital fossa. He had tender axillary adenopathy. The patient started a new job when he returned from Haiti, working 4 days per week in a greenhouse. He denied any fever, chills, or night sweats. He was in good health without any underlying chronic health problems. Physical examination revealed a small eschar over the distal phalanx of the patient's right fifth finger (Figure 1). There were 2 erythematous nodules over the extensor surface of his right forearm (Figure 2) as well as over the antecubital fossa (Figure 3). A punch biopsy was performed and results showed suppurative granulomatous dermatitis. Sporothrix schenckii was grown from the specimen.

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Cryptococcosis in the University Hospital, Kuala Lumpur and review of published cases

Pathmanathan

Soon

1982

Transactions of the Royal Society of Tropical Medicine and Hygi

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Between January 1974 and June 1980, 85 cases of cryptococcosis were diagnosed in the University Hospital, Kuala Lumpur, Malaysia. The diagnosis was based on positive culture of the organism in 81 cases; the remaining four were diagnosed on histopathological findings. Cerebral cryptococcosis was the most common presentation and Chinese are particularly susceptible (72% of cases). The incidence of the disease is shown to be far greater than previously suspected. Association with compromised host status is uncommon (14%). The local literature is briefly reviewed and the findings discussed.

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Immunoperoxidase localization of Sporothrix schenckii and Cryptococcus neoformans. Staining of tissue sections fixed in 4% formaldehyde solution and embedded in paraffin

Cited by 9 publications

References 0 publications

Fungal Infections

Fungal Infections

A Rose by Any Other Name

Cryptococcosis in the University Hospital, Kuala Lumpur and review of published cases

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