Immunopathogenesis of Psoriasis: News in an Old Concept

Guilhou, J J

doi:10.1159/000018022

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…Co-localization of T-cell markers has major advantages, above single-staining cutaneous T cells using immunoperoxidase histochemistry. Our observation of increased CD4+ CD25+, CD4+ CD45RO+, CD8+ CD25+ and CD8+ CD45RO+ T cells in psoriatic skin is an independent confirmation of the results of other studies and suggests that the immunofluorescence method is appropriate [1, 31, 32]. …”

Section: Discussionsupporting

confidence: 89%

“…Therefore, we aimed to cluster the three most common markers for T reg (CD4, CD25, Foxp3) to identify T reg . To demonstrate co-localization of these markers a quantitative immunofluorescence technique (Zenon) was used [30,31,32]. With this fast technique, primary antibodies can be combined with fluorescence-labeled Fab fragments of secondary antibodies directed against their Fc regions.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Lesional CD4+ CD25+ Foxp3+ Regulatory T Cells in Psoriasis

et al. 2006

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Background: Depletion of CD4+ CD25+ Foxp3+ naturally occurring regulatory T cells (T_reg) induces autoimmune phenomena. These cells have not yet been fully characterized in the skin of psoriatic patients. Objectives: To prove that the Zenon immunofluorescent labeling technique is suitable for the demonstration of co-localization of T-cell markers and in particular to show the distribution of T_reg in psoriatic skin. Methods: In biopsies derived from normal and psoriatic skin, CD4+ CD25+, CD4+ CD45RO+, CD8+ CD25+, CD8+ CD45RO+ and CD4+ CD25+ Foxp3+ cells in the dermis and in the epidermis were immunophenotyped, using a quantitative immunofluorescent labeling technique (Zenon), analyzed and compared using image analysis. Results:The immunofluorescent labeling technique was shown to be an easy and reliable tool to demonstrate co-localization of T-cell markers. In psoriasis, all pathogenic T-cell subsets (CD4+ CD25+, CD4+ CD45RO+, CD8+ CD25+ and CD8+ CD45RO+ cells) were significantly increased in the dermis and in the epidermis, as compared to normal skin (all p < 0.05). Using this labeling technique we were able to reveal CD4+ CD25+ Foxp3+ T_reg in psoriatic dermis, but not in the dermis of normal skin (p < 0.0001). Conclusions:The Zenon immunofluorescence technique in combination with image analysis is suitable for the demonstration of co-localization of T-cell markers in tissue. Increased numbers of pathogenic T cells (CD4+ CD25+, CD4+ CD45RO+, CD8+ CD25+ and CD8+ CD45RO+) were shown in the dermis and epidermis, whereas CD4+ CD25+ Foxp3+ T_reg were identified in psoriatic skin with a predilection for the upper dermis.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Identification of Lesional CD4+ CD25+ Foxp3+ Regulatory T Cells in Psoriasis

et al. 2006

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“…One of the most likely hypotheses is that it is related to intrinsic keratinocyte abnormalities associated with immune disturbance [9]. Skin-infiltrating lymphocytes, in fact, play an important role in the induction and maintenance of keratinocyte hyperproliferation in both involved psoriatic skin and nails.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Psoriatic Nails with Topical Cyclosporin: A Prospective, Randomized Placebo-Controlled Study

et al. 2003

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Background: Nail involvement is a frequent event in the course of psoriasis causing severe distress. While systemic cyclosporin (CsA) represents a well-established therapy of psoriasis, its topical use is limited by the difficult penetration of the molecule through the skin and the nail because of its highly lipophilic nature. Objectives: We carried out a prospective randomized placebo-controlled study in order to analyze the effectiveness and tolerability of topical oil-dissolved 70% CsA solution in nail psoriasis. Methods: Sixteen adult patients with nail psoriasis, divided randomly into two groups of 8 patients (group A and group B), were treated respectively with a 70% maize-oil-dissolved oral CsA solution and maize oil alone. To compare the therapeutic effectiveness, all patients were evaluated, before starting the treatment and after 12 weeks of therapy, by the same dermatologists. The patients were also asked to assess the severity of their nail involvement at baseline and at the end of the treatment. Results: In group A, 3 patients came to a complete resolution of nail lesions and 5 showed a substantial improvement of the overall severity score. In group B, a slight improvement was noted in only 1 patient. All the patients of group A judged positively the results of the therapy, while in group B only 1 patient reported a moderate improvement. Conclusion: Our results show that topical therapy with oral CsA solution is a safe, effective and cosmetically highly acceptable treatment modality for nail psoriasis. The ability of CsA to influence keratinocyte proliferation and T-cell lymphokine release, reducing the cornification of the upper layers of the epidermis, may prevent the typical alterations observed in nail psoriasis.

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“…субпопуляций) в патогенезе заболевания подтверж-дается участием их в формировании псориатическо-го инфильтрата. Оценка состояния иммунной си-стемы позволяет наиболее информативно охаракте-ризовать стадии активности патологического про-цесса при псориазе, типы его течения и определить наиболее эффективные методы терапии [2][3][4][5][6][7].…”

Section: ключевые слова: карталин псориаз лечение липиды иммуногиunclassified

Psoriasis: a new solution to the old problems

Dmitruk¹,

N²

2014

Klin. dermatol. venerol.

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Immunopathogenesis of Psoriasis: News in an Old Concept

Cited by 14 publications

References 29 publications

Identification of Lesional CD4+ CD25+ Foxp3+ Regulatory T Cells in Psoriasis

Identification of Lesional CD4+ CD25+ Foxp3+ Regulatory T Cells in Psoriasis

Treatment of Psoriatic Nails with Topical Cyclosporin: A Prospective, Randomized Placebo-Controlled Study

Psoriasis: a new solution to the old problems

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