Immunomodulatory effects of human amniotic epithelial cells on naive CD4+ T cells from women with unexplained recurrent spontaneous abortion

Motedayyen, Hossein; Zarnani, Amir‐Hassan; Tajik, Nader; Ghotloo, Somayeh; Rezaei, Abbas

doi:10.1016/j.placenta.2018.06.008

Cited by 43 publications

(30 citation statements)

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“…The dose-dependency of the immunoregulatory capacity of HAECs on different immune cells have been highlighted before (17)(18)(19); it seems that HAECs exert their effect on phagocytosis and oxidative burst of neutrophils in a dose-dependent manner too. But none of neutrophil functions evaluated here replicate exactly the results of past researches in which usually progressive dilution of HAECs numbers are accompanied with less pronounced inhibition on leukocytes (17)(18)(19). As it is seen (in the Results section), when HAEC：neutrophil ratio was highest (1：1), the level of respiratory burst did not differ significantly.…”

Section: Discussionmentioning

confidence: 97%

“…In the present study, therefore, the modulation of two important neutrophil functions, namely respiratory burst and phagocytosis, by HAECs have been addressed in vitro. As past studies have shown that the effect of HAECs are time and dose-dependent (17)(18)(19), here we examined the effect of different number of HAECs on the neutrophil functions at different times; after 24 and 48 hours of coculturing.…”

Section: Discussionmentioning

confidence: 99%

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Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Alipour

Motedayyen

Sereshki³

et al. 2020

IJSC

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Background and Objectives: As a stem cell group, Human amniotic epithelial cells (HAECs) have numerous advantages over their embryonic and adult counterparts for therapeutic utility. They are closer to clinical applications compared to other stem cell types. Additionally, the anti-inflammatory and immunoregulatory properties of HAECs toward several immune cells have been shown previously. Nevertheless, despite the ever-increasing importance of neutrophils in the immune and non-immune processes, a few studies investigated the interaction of neutrophils and HAECs. To increase the current knowledge of HAECs immunology which is necessary for optimizing their future clinical applications, here we explored the effect of HAECs on two chief neutrophil functions; respiratory burst and phagocytosis. Methods and Results: Freshly isolated human blood neutrophils were co-cultured with different number of HAECs for about 24 or 48 hours, then the oxidative burst and phagocytosis of stimulated neutrophils were assessed and compared. The results demonstrated a substantial elevation in the phagocytosis percentage, conversely a significant reduction in the oxidative burst of HAECs-cocultured neutrophils. These effects were dose-dependent, but did not show similar patterns. Likewise, the elongation of coculture period inversely influenced the HAECs-induced effects on the two neutrophil functions. Conclusions: The present study, for the first time, investigated the HAECs-mediated effects on the two main neutrophil functions. The findings suggest that HAECs by enhancement of phagocytic ability and simultaneously, attenuation of oxidative burst capacity of neutrophils protect the fetus from both microbial treats and oxidative stress and their consequent inflammation; thus corroborate the current anti-inflammatory vision of HAECs.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Alipour

Motedayyen

Sereshki³

et al. 2020

IJSC

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“…They promote T-cell differentiation toward the Treg phenotype, which in turn induces a phenotype switch from M1 to M2 macrophages with tissue repair function [43,44]. AECs also release anti-inflammatory cytokines and proteins, including IL-1 receptor antagonist, tissue inhibitors of matrix metalloproteinases − 1, -2, -3, and − 4, TGF-β and IL-10 [44,45]. These soluble factors contribute to the regulation of macrophage recruitment and inhibit the chemotactic activity of neutrophils and macrophages [46].…”

Section: The Rationale For Human Placentaand Amnion-derived Cell Therapymentioning

confidence: 99%

Treatment of COVID-19 Pneumonia: the Case for Placenta-derived Cell Therapy

Berishvili

Kaiser

Cohen

et al. 2020

Stem Cell Rev and Rep

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Nearly 500'000 fatalities due to COVID-19 have been reported globally and the death toll is still rising. Most deaths are due to acute respiratory distress syndrome (ARDS), as a result of an excessive immune response and a cytokine storm elicited by severe SARS-CoV-2 lung infection, rather than by a direct cytopathic effect of the virus. In the most severe forms of the disease therapies should aim primarily at dampening the uncontrolled inflammatory/immune response responsible for most fatalities. Pharmacological agents-antiviral and anti-inflammatory molecules-have not been able so far to achieve compelling results for the control of severe COVID-19 pneumonia. Cells derived from the placenta and/or fetal membranes, in particular amniotic epithelial cells (AEC) and decidual stromal cells (DSC), have established, well-characterized, potent anti-inflammatory and immune-modulatory properties that make them attractive candidates for a cell-based therapy of COVID19 pneumonia. Placenta-derived cells are easy to procure from a perennial source and pose minimal ethical issues for their utilization. In view of the existing clinical evidence for the innocuousness and efficiency of systemic administration of DSCs or AECs in similar conditions, we advocate for the initiation of clinical trials using this strategy in the treatment of severe COVID-19 disease.

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“…However, with 50% of RSA still occurring due to unknown causes, we designate this as unexplained recurrent abortion (URSA) 1 . URSA is an excluded diagnostic disease, and its diagnosis and treatment still face great challenges, although studies have shown that the underlying causes of RSA include systemic endocrine and immune disorders 1,[4][5][6][7][8] . Many scholars are now paying close attention to the gamete and the regulation of maternal-fetal interactions after blastocyst implantation [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Mechanism of LIN28B in trophoblastic villous cells of unexplained recurrent abortion

Huang

Niu

Song

et al. 2020

Preprint

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Background: LIN28B plays an important role in early embryonic development, but its role in villous trophoblast implantation and differentiation remains unknown. To verify the role of LIN28B in trophoblastic villous tissue and cells from women with URSA(unexplained recurrent spontaneous abortion)and artificial termination of pregnancy (negative control, NC). Methods:The Lin28b gene and its protein expression level were detected with real-time quantitative PCR, Western immunoblotting analysis, and immunocytochemistry. The gene was also overexpressed in chorionic villous cell lines (HTR-8/SVneo and BeWo) to examine its effect on trophoblast function.Results: The expression of LIN28B mRNA and protein of URSA villi was lower than that in the NC group. At the cellular level, overexpression of LIN28B enhanced cellular migration, and invasion, and inhibited apoptosis. LIN28B may inhibit apoptosis by promoting Akt phosphorylation and by inhibiting Bad phosphorylation and Bcl-2 expression. In addition, LIN28B inhibited cell fusion and reduced cellular syncytia. Conclusions: LIN28B can inhibit cell proliferation, invasion and migration in vitro, and promote apoptosis and fusion. The low expression of LIN28B in URSA villous trophoblast cells may be one of the causes of abortion. The role of LIN28B in villous trophoblasts needs further study.

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Immunomodulatory effects of human amniotic epithelial cells on naive CD4+ T cells from women with unexplained recurrent spontaneous abortion

Cited by 43 publications

References 36 publications

Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Treatment of COVID-19 Pneumonia: the Case for Placenta-derived Cell Therapy

Mechanism of LIN28B in trophoblastic villous cells of unexplained recurrent abortion

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