Immunomodulatory effects of egg white peptides on immunosuppressed mice and sequence identification of immunomodulatory peptides

Chen, Mengwei; Zhang, Fan; Su, Yujie; Chang, Cuihua; Li, Junhua; Gu, Luping; Yang, Yanjun

doi:10.1016/j.fbio.2022.101873

Cited by 15 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al . (2022) reported a best inhibitory effect on LYM% decrease observed at medium dose of egg white peptides, while Gao et al . (2019) revealed no typical dose–response pharmacodynamics of yak bone hydrolysates.…”

Section: Resultsmentioning

confidence: 99%

“…Chen et al . (2022) have demonstrated that oral administration of 750 mg kg −1 d −1 egg white peptides alleviated leucocytes reduction.…”

Section: Resultsmentioning

confidence: 99%

“…The leucocyte number of mammals was demonstrated to decrease significantly within 6 h after LPS injection and gradually returned to normal level after 12 h (Opal et al, 1999). Chen et al (2022) have demonstrated that oral administration of 750 mg kg −1 d −1 egg white peptides alleviated leucocytes reduction.…”

Section: Measurement Of Peripheral Blood Cellsmentioning

confidence: 98%

See 2 more Smart Citations

Identification of peptides from protease‐fermented milk protein and immunomodulatory effect in vivo against lipopolysaccharide‐induced inflammation

Xie

Zhang

2022

Int J of Food Sci Tech

View full text Add to dashboard Cite

Food-derived peptides exhibit potential immunomodulatory effects and become popular functional ingredients in food industry. However, the in vivo effect varies from in vitro while the optimal utilisation content remains unclear. This study aimed to identify novel peptides from protease-facilitated fermentation, to evaluate their immunomodulatory effect and effect-dose relationship in vivo. Totally 2045 peptides were screened by liquid chromatography mass spectrometry (LC-MS/MS), and in silico methods were applied to predict the potential bioactivity through Peptide Ranker, GRAVY Calculator and PepDraw. In vivo assay indicated that 200 mg kg −1 milk-derived peptides showed the optimal regulation on lipopolysaccharide-induced inflammation by enhancing the immune function of spleen and regulating immunoglobulin and cytokine production. These peptides contributed to the prevention of inflammation through the oral route and could be further applied in functional food development.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Chen et al . (2022) have demonstrated that oral administration of 750 mg kg −1 d −1 egg white peptides alleviated leucocytes reduction.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of peptides from protease‐fermented milk protein and immunomodulatory effect in vivo against lipopolysaccharide‐induced inflammation

Xie

Zhang

2022

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…In our previous study, the albumin peptides obtained by pre-heat treatment and bienzymatic hydrolysis had higher antioxidant activity as compared with the ones by pre-heat treatment and single enzymatic hydrolysis and bienzymatic hydrolysis alone, and their molecular weight range was between 200 and 1000 Da. It has also been reported that peptides with molecular weight between 500 and 1000 Da have high antioxidant activity and good transdermal, hygroscopic, and moisturizing properties. , …”

Section: Resultsmentioning

confidence: 99%

“…HPLC–ESI-MS/MS (MALDI SYNAPT MS, Waters, American) was used to identify the structure of characteristic selenopeptide sequences. Instrument parameters were set on the basis of our previous experiments …”

Section: Methodsmentioning

confidence: 99%

Identification and Immunomodulatory Effect on Immunosuppressed Mice of Selenium-Enriched Peptides of Egg White

Chen

Zhang

et al. 2022

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Selenium-enriched egg white peptides (Se-EWP) were prepared by pre-heat treatment and enzymatic hydrolysis in this study. In addition, their selenopeptide sequence identification and immunomodulatory effect were investigated. Results showed that the yield of Se-EWP obtained from alkaline–neutral protease treatment reached 76.90%, and peptides with a molecular weight of 200–1000 Da accounted for 98.33%. Four characteristic selenopeptides, including SeCys-Trp-Leu-Glu, Trp-Ser-SeCys, SeMet-Ala-Pro, and SeMet-Leu, were identified by HPLC–ESI-MS/MS, which were rich in hydrophobic and branched-chain amino acids. Se-EWP (750 mg/kg/d) could effectively retard the decrease of immune organ index in immunosuppressed mice induced by cyclophosphamide. Moreover, supplementation of Se-EWP could promote a higher content of Se in liver, the number of white blood cells, and the levels of serum cytokines (IL-6, IL-2, and TNF-α) as compared with EWP groups, indicating that Se-EWP could effectively alleviate immunosuppression induced by cyclophosphamide. These findings suggested that Se-EWP exhibited great potential as functional foods for immunomodulatory effect.

show abstract

Immunomodulatory Function of Egg White Peptides in RAW264.7 Macrophage Cells and Immunosuppressive Mice Induced by Cyclophosphamide

Zhang

Chang

et al. 2022

Int J Pept Res Ther

View full text Add to dashboard Cite

Immunomodulatory effects of egg white peptides on immunosuppressed mice and sequence identification of immunomodulatory peptides

Cited by 15 publications

References 30 publications

Identification of peptides from protease‐fermented milk protein and immunomodulatory effect in vivo against lipopolysaccharide‐induced inflammation

Identification of peptides from protease‐fermented milk protein and immunomodulatory effect in vivo against lipopolysaccharide‐induced inflammation

Identification and Immunomodulatory Effect on Immunosuppressed Mice of Selenium-Enriched Peptides of Egg White

Immunomodulatory Function of Egg White Peptides in RAW264.7 Macrophage Cells and Immunosuppressive Mice Induced by Cyclophosphamide

Contact Info

Product

Resources

About