Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study

Abstract

This study was conducted to assess the in vivo and ex vivo immunomodulatory effect of the ethanol leaves extract of Moringa peregrina in Balb/c mice. For this study, five groups of 5 Balb/c mice were given a single acute subtoxic oral dose of the ethanolic extract at 1.13, 11.30, 23.40 and 113.4 mg/kg and the immunomodulatory effect was assessed on the 6th day following the ingestion. In the (non-functional) assessment, the effect of the extract on the body weight, relative lymphoid organ weight, splenic cellularity and peripheral blood hematologic parameters were evaluated. While in the immunomodulation assessment (functional), we investigated the effect of the extract on the proliferative capacity of splenic lymphocytes and peripheral T and B lymphocytes using mitogen blastogenesis, mixed allogeneic MLR and IgM-Plaque forming cells assays. The ingestion of M. peregrina extract caused a significant increase in the body weight, weight and number of cells of spleen and lymph nodes of the treated mice. Furthermore, the count of RBCs, WBCs, platelets, hemoglobin concentration and PCV % were increased by the extract treatment in a dose-dependent manner. M. peregrina enhanced the proliferative responses of splenic lymphocytes for both T cell and B-cell mitogens. Likewise, the mixed lymphocyte reaction MLR assay has revealed a T-cell dependent proliferation enhancement in the extract treated mice. Moreover, the oral administration of M. peregrina leaves extracts significantly increased PFCs/106 splenocytes in a dose-dependent manner. In conclusion, subtoxic acute doses of M. peregrina extract demonstrated significant potential as an immunomodulatory agent even at the lowest dose of 1.13 mg/kg.

Antibacterial and immunomodulatory activity of moringa ( Moringa oleifera ) seed extract in Longfin yellowtail ( Seriola rivoliana ) peripheral blood leukocytes

“…When cells die, they rapidly lose the ability to convert the substrate to the product (blue colour) (Figure 5b). Al‐Majali et al, (2017) showed in an in vivo study that ethanolic extract increased spleen cell number with oral administration of Moringa peregrina leaves. Moreover, this proliferation effect could have been the result of lymphocyte migrations from the spleen to the circulatory system.…”

“…MoSE) with viable cells results in a signal proportional to the number of viable cells found in the culture (pink colour). When cells die, they rapidly lose the ability to convert the substrate to the product (blue colour) (Figure5b) Al-Majali et al, (2017). showed in an in vivo study that ethanolic extract increased spleen cell number with oral administration of Moringa peregrina leaves.…”

Phytochemical analysis, antioxidant, and antimicrobial activities of Jordanian Pomegranate peels

“…Generally, extracts with high phenolic content and high antioxidant activities also exhibit high antimicrobial activities [ 46 , 54 , 55 ]. The ethanol and methanol extracts of the pomegranate peels showed the highest inhibition activity against the microbial strains Pseudomonas aeruginosa , Klebsiella pneumoniae , and Micrococcus luteus and the fungal strain Candida albicans .…”

Effect of Moringa Olifera Supplementation on Immune Response, Oxidative Stress in Barki Rams

“…Similar results were obtained in rabbits (Jiwuba et al, 2016, El-Badawi et al al., 2016, El-Gindy et al, 2017& salem et al, 2020 and in rats (Adedapo et al, 2005, Gupta et al, 2010, Isitua & Ibeh, 2013and Asomugha et al, 2015 but the increase in WBC count was stastically significant. Also, Al-Majali et al (2017) showed that supplementation of ethanolic Moringa peregrina extract to rats resulted in a significant increase in total WBC counts in peripheral blood. Lamou et al, 2016, Kasolo et al, 2010 attributed the increase in WBCs to decreased oligosaccharides in Moringa oleifera leaves.…”

“…Previous research reports concluded that M. oleifera is an immunomodulatory agent that can stimulate the production of IgG, IgM and IgA (Al-Badawi et al, 2016, El Gendy et al, 2017, Al-Majali et al, 2017and Abd-Elhakim et al, 2018 as well as the aqueous extract of M. oleifera showed a strong immune-modulating effect that could modulate B-cell activation and stimulate the production of IgM, IgA and IgG level after administration (Ojeka et al 2016), and T cells where it will result for induction of IL 10 production (Fard et al 2015;Tan et al 2015) and inhibit production of TNFα, IL-6, IL-8 (Kooltheat et al 2014) andIL-2 (Sashidhara et al 2009).…”