Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes

Sawant, Pradeep Mahadev; Verma, Pawan Kumar; Subudhi, P.K.; Chaturvedi, Uttara; Singh, Mithilesh; Kumar, Rajeev Ranjan; Tiwari, Ashok Kumar

doi:10.1016/j.vetimm.2011.07.006

Cited by 53 publications

(35 citation statements)

References 42 publications

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“…Chicks from eggs injected with IL-4 containing plasmids were found to have enhanced anti-coccidia immune responses [12] and had larger caecal tonsils, increased numbers of CD8+ cells in the caecal tonsils and a higher CCR9 mRNA level in caecal tonsils compared to control chicks [10]. Lastly, co-administration of IL-4 to chickens in a plasmid vector (pVIVO2) also containing two genes of Newcastle disease virus as a DNA vaccine (injected intramuscularly on three occasions), led to higher levels of IgY and higher protection (40% vs 10%) compared to the DNA vaccine without IL-4 [13], suggesting it may have value as a molecular adjuvant.…”

Section: Il-4 Il-13 and Il-4/13 Moleculesmentioning

confidence: 96%

The evolution of IL-4 and IL-13 and their receptor subunits

Wang

Secombes

2015

Cytokine

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This review will outline what is known about the origins and evolution of type 2 cytokines and their receptors in vertebrates. It takes advantage of the recent advances made in gene identification from the many vertebrate genomes that have now been sequenced. It will also describe what functional studies have been performed to date, giving clues to the role of these molecules and signalling pathways in non-mammalian vertebrates.

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Section: Il-4 Il-13 and Il-4/13 Moleculesmentioning

confidence: 96%

The evolution of IL-4 and IL-13 and their receptor subunits

Wang

Secombes

2015

Cytokine

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“…However, infants often do not reap the same protective benefits from vaccination as older children and adults due to inefficient or suppressive innate and adaptive host immunity [37, 38]. A growing number of studies have reported safe and effective antiviral activity with vaccine-mediated enhancement of pro-inflammatory cytokines [10, 11, 14, 39–43], yet less is known about direct immune stimulation. IFNγ and GM-CSF have been identified as critical cytokines for the protection against such organisms as RSV, influenza, rhinovirus, and tuberculosis [44–48], yet there remains little information describing direct innate immune stimulation in the virally-infected infant airway.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophage-activating cytokines such as interferon gamma (IFNγ) and granulocyte colony-stimulating factor (GM-CSF) are commercially available immune potentiators used to prevent infections in patients with chronic granulomatous disease and febrile neutropenia, respectively [7–9]. Moreover, an increasing number of reports describe the protective function of IFNγ and GM-CSF as vaccine adjuvants [10–13] [14, 15]. Despite the reported anti-viral activity, systemic toxicity with immune potentiating cytokines discourages their use in vulnerable patient populations, including young children.…”

Section: Introductionmentioning

confidence: 99%

Age predicts cytokine kinetics and innate immune cell activation following intranasal delivery of IFNγ and GM-CSF in a mouse model of RSV infection

et al. 2017

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Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in young children and is further associated with increased healthcare utilization and cost of care in the first years of life. Severe RSV disease during infancy has also been linked to the later development of allergic asthma, yet there remains no licensed RSV vaccine or treatment. Pre-clinical and clinical studies have shown that disease severity and development of allergic asthma are associated with differences in cytokine production. As a result, stimulation of the innate host immune response with immune potentiators is gaining attention for their prospective application in populations with limited immune responses to antigenic stimuli or against pathogens for which vaccines do not exist. Specifically, macrophage-activating cytokines such as interferon gamma (IFNγ) and granulocyte colony-stimulating factor (GM-CSF) are commercially available immune potentiators used to prevent infections in patients with chronic granulomatous disease and febrile neutropenia, respectively. Moreover, an increasing number of reports describe the protective function of IFNγ and GM-CSF as vaccine adjuvants. Although a positive correlation between cytokine production and age has previously been reported, little is known about age-dependent cytokine metabolism or immune activating responses in infant compared to adult lungs. Here we use a non-compartmental pharmacokinetic model in naïve and RSV-infected infant and adult BALB/c mice to determine the effect of age on IFNγ and GM-CSF elimination and innate cell activation following intranasal delivery.

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“…[20][21][22] Therefore, in this research, the DNA plasmid pIRES-HN/F coexpressing F and HN proteins was constructed and used for in ovo immunization against NDV. Immunization with 40 µg of pIRES-HN/F per egg induced a significant level of antibody titer; however, it was not effective in protecting against the virus challenge.…”

Section: Discussionmentioning

confidence: 99%

Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease

Firouzamandi¹,

Moeini²,

Hosseini³

et al. 2016

IJN

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Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer ( P <0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 μg pDNA + 64 μg D-SPM and 40 μg pDNA at 4 weeks postvaccination ( P >0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.

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Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes

Cited by 53 publications

References 42 publications

The evolution of IL-4 and IL-13 and their receptor subunits

The evolution of IL-4 and IL-13 and their receptor subunits

Age predicts cytokine kinetics and innate immune cell activation following intranasal delivery of IFNγ and GM-CSF in a mouse model of RSV infection

Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease

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