Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B<sub>1</sub> in agricultural products

Li, Ming; Zhang, Yuanyuan; Zhao, Ru-Jin; Liu, Zhenjiang; Xia, Hong; Cui, Yin; Xue, Yan; Du, Daolin

doi:10.1039/c8ra04460k

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…The biotinylated antibody probe and magnetic probe were prepared in order to develop the MBI method. The biotinylated antibody probe was prepared by using the carbodiimide method [ 31 , 32 ]. The anti-BBP PcAb (dialyzed for 4 h in 0.1 mol/L CBS buffer, pH 9.2) was mixed with BNHS (3.8 mg in 100 μL DMSO), then incubated at 25 °C for 6 h. After dialysis in 0.01 mol/L PBS buffer overnight at 4 °C, the prepared biotinylated antibody probe was stored in 0.01 mol/L PBS buffer containing 50% glycerol and 3% bovine serum albumin.…”

Section: Methodsmentioning

confidence: 99%

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Xia

Cui

et al. 2022

Biosensors

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A magnetic-based immunoassay (MBI) combined with biotin-streptavidin amplification was proposed for butyl benzyl phthalate (BBP) investigation and risk assessment. The values of LOD (limit of detection, IC10) and IC50 were 0.57 ng/mL and 119.61 ng/mL, with a detection range of 0.57–24977.71 ng/mL for MBI. The specificity, accuracy and precision are well demonstrated. A total of 36 environmental water samples of urban sewage from Zhenjiang, China, were collected and assessed for BBP contamination. The results show that BBP-positive levels ranged from 2.47 to 89.21 ng/mL, with a positive rate of 77.8%. The health effects of BBP in the urban sewage were within a controllable range, and the ambient severity for health (ASI) was below 1.49. The highest value of AS for ecology (ASII) was 7.43, which indicates a potential harm to ecology. The entropy value of risk quotient was below 100, the highest being 59.47, which poses a low risk to the environment and ecology, indicating that there is a need to strengthen BBP controls. The non-carcinogenic risk of BBP exposure from drinking water was higher for females than that for males, and the non-carcinogenic risk from drinking-water and bathing pathways was negligible. This study could provide an alternative method for detecting BBP and essential information for controlling BBP contamination.

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Section: Methodsmentioning

confidence: 99%

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Xia

Cui

et al. 2022

Biosensors

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“…To develop a highly effective and sensitive BAS-MBI for ZEN, two immunoprobes of magnetic antigen probe and biotinylated antibody probe were prepared. Firstly, the magnetic antigen probe was prepared through the coupling of magnetic-particles and ZEN-antigen by NHS/EDC activation method [29,31,32]. Well-dispered surface carboxyl-functional group-modified magnetic-particles (50 μL) were created with sulfo-NHS (19.8 mg in 200 μL H 2 O) and EDC (10.2 mg in 200 μL H 2 O) in MES buffer (550 μL) and were activated for 30 min at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Magnetic bead immunoassay (MBI), a potential homogeneous assay which provides a fully reaction interface in magnetic particles as compared to the microtiter plate-based method, could be achieved with efficient magnetic separation and a convenient detection procedure [27,28]. The biotin–streptavidin system (BAS) is an ideal strategy to realize the amplification of signal and improvement of sensitivity [29]. With the help of the BAS, the labeling tracer amount of immunoassay can be significantly increased and the detection signal is more obvious, thus ensuring higher sensitivity and avoiding the use of a tracer-labeled second antibody [30].…”

Section: Introductionmentioning

confidence: 99%

Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay

Yang

Mao

et al. 2019

Toxins

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In total, 405 samples of corn, corn products, and swine feed from China in 2016–2018 were surveyed for zearalenone (ZEN) contamination using a magnetic bead immunoassay-coupled biotin–streptavidin system (BAS-MBI). The developed BAS-MBI had a limit of detection (LOD) of 0.098 ng mL−1, with half-maximal inhibition concentration (IC50) of 0.71 ng mL−1 in working buffer, and an LOD of 0.98 ng g−1; the detection range was from 0.98 to 51.6 ng g−1 in authentic agricultural samples. The BAS-MBI has been demonstrated to be a powerful method for the rapid, sensitive, specific, and accurate determination of ZEN. The ZEN positivity rate reached the highest level of 40.6% in 133 samples in 2016; ZEN levels ranged from 1.8 to 1100.0 ng g−1, with an average level of 217.9 ng g−1. In 2017, the ZEN positivity rate was the lowest at 24.5% in 143 samples; ZEN levels ranged from 1.1 to 722.6 ng g−1, with an average of 166.7 ng g−1. In 2018, the ZEN positivity rate was 31.8% in 129 samples; ZEN levels ranged from 1.3 to 947.8 ng g−1, with an average of 157.0 ng g−1. About 20% of ZEN-positive samples exceeded maximum limit levels. An alternative method of ZEN detection and a valuable reference for ZEN contamination in corn and its related products in China are provided. This survey suggests the need for prevention of serious ZEN contamination, along with management for food safety and human health.

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“…In recent years, magnetic nanoparticles based technology with multidisciplinary and remarkable characteristics offers great possibilities for development for new analytical methodology [24–26]. Immunomagnetic beads (IMBs) due to the distinct physical and chemical properties, such as uniform diameter particles, large surface area, simplicity, superior compatibility, good sensitivity, and easy operation that can be homogenously distributed in colloidal suspension, have been widely applied in various fields, including nucleic acid extraction, peptide and protein analysis, and antibody purification [27–31]. In addition, IMBs have good biocompatibility for coupling with antibodies or small molecules and larger surface area than the flat base of a microtiter plate, which immobilizes more numbers of “active molecules” and enhances the sensitivity of the immunoassay.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

Zhang

Liu

et al. 2020

J of Separation Science

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We aimed to establish an automated versatile sample preconcentration method based on the modified immunomagnetic beads, which was utilized to enrich for aflatoxin B1 from the matrices. The critical main parameters affecting the extraction efficiency, such as usage amount of immunomagnetic beads, reaction time, elution time, and blending way were investigated. Under the optimized conditions, the content of aflatoxin B1 was analyzed by high‐performance liquid chromatography, the mobile phase consists of water–acetonitrile–methanol (42:18:10, v/v/v), and fluorescence detection was performed with excitation and emission wavelengths at 360 and 440 nm, respectively. Moreover, the performance of preconcentration method was compared with the conventional method based on the immunoaffinity column. The accuracy of two clean‐up methods was within the error range. In addition, the stability and recyclability of the immunomagnetic beads was studied by recycling them five times. The results for the respective analysis in various samples demonstrated that the developed extraction platform provides a promising approach that is simple, rapid, sensitive, and easy to use.

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Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B₁ in agricultural products

Abstract: A strategy of immunomagnetic beads coupled with biotin-streptavidin system (BAS-IMB) for detecting AFB1 was suggested and applied in agricultural products.

Cited by 14 publications

References 24 publications

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

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Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products

Abstract: A strategy of immunomagnetic beads coupled with biotin-streptavidin system (BAS-IMB) for detecting AFB1 was suggested and applied in agricultural products.

Cited by 14 publications

References 24 publications

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Butyl Benzyl Phthalate in Urban Sewage by Magnetic-Based Immunoassay: Environmental Levels and Risk Assessment

Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

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Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B₁ in agricultural products