Immunolocalization of substance P in human skin mast cells

Toyoda, Masahiko; Makino, Teruhiko; Kagoura, Masayori; Morohashi, Masaaki

doi:10.1007/s004030000149

Cited by 55 publications

(44 citation statements)

References 17 publications

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“…On histopathological examination, hyperkeratosis, acanthosis and infiltration of mast cells and eosinophils were apparent in the lesional skin, and the pathological appearance of PiCl-treated NC/Nga mice was essentially the same as that of conventional NC/Nga mice reported previously [1, 2, 3]. Furthermore, the findings correspond well with typical symptoms observed in patients with AD, such as an increase in mast cells or eosinophil infiltration at the site of lesions [16, 17]. …”

Section: Discussionsupporting

confidence: 73%

“…SP has been implicated in particular, because increased numbers of nerve fibres containing SP have been found concomitantly with a decrease in cutaneous levels of SP in humans [7, 8, 9, 16]. SP is reported to exist in cutaneous mast cells and to be released not only from neurons, but also from immune cells such as mast cells [16]or monocytes [17]. Patients with AD have been found to have significantly increased plasma levels of SP compared with healthy controls [24].…”

Section: Discussionmentioning

confidence: 99%

“…It is also known that AD can be exacerbated or easily induced in genetically predisposed individuals by psychological stress [12, 13, 14], due to the release of SP [15]. Toyoda et al [16]recently demonstrated that the number of SP-containing mast cells, as well as the amount of SP within mast cells, was increased in the lesional skin of AD patients. Taken together, these data suggest that SP may play an etiological role in the mechanisms underlying AD.…”

Section: Introductionmentioning

confidence: 99%

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Role of Substance P in an NC/Nga Mouse Model of Atopic Dermatitis-Like Disease

Ohmura¹,

Tsunenari

Hayashi³

et al. 2004

Int Arch Allergy Immunol

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Background: Atopic dermatitis (AD) can be exacerbated or induced in genetically predisposed individuals by psychological stress, which causes the release of substance P (SP). Therefore, SP may play an etiological role in the mechanisms underlying AD. Methods: Changes in the number of mast cells and SP-containing mast cells in lesional skin, and the serum concentrations of SP and IgE during the development of AD-like disease up to 8 weeks after the start of picryl chloride (PiCl) induction in NC/Nga mice were examined. Results: Clinical signs and symptoms seen in PiCl-treated NC/Nga mice as a model of AD-like disease began with erythema and haemorrhage, followed by oedema, superficial erosion, deep excoriation, scaling and dryness of the skin, as well as retarded growth, and the changes were exacerbated with an increase in the number of PiCl applications. An increase in the number of mast cells and eosinophil infiltration was observed in the lesional skin. The increase in SP-positive mast cells in the dermis in this model was significant from 1 week after the start of induction treatment, compared with intact mice, and SP-positive nerve fibres were observed in the dermis. Conclusion: SP is a crucial mediator of both dermatitis and scratching behaviour in this model.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Substance P in an NC/Nga Mouse Model of Atopic Dermatitis-Like Disease

Ohmura¹,

Tsunenari

Hayashi³

et al. 2004

Int Arch Allergy Immunol

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“…Therefore, signals could be transmitted by nexus ion channel contact between mast cells and muscle cells -most probably via substances like Ca 2+ , Mn 2+ [76], K + [77], Cl - [78,79], enzymes [73], biologically active substances as the vasoactive intestinal polypeptide -VIP [36,37], histamine [13,73,80], and substance P [81], all of them responsible for signal transduction.…”

Section: Some Morphofunctional Aspects Of the Interaction Between Smomentioning

confidence: 99%

Structure and Function of Smooth Muscle with Special Reference to Mast Cells

Vodenicharov¹

2012

Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues - From Molecules to Humans

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“…Immunohistochemical study demonstrated that most of venules around the sebaceous glands not in normal subjects but in acne patients expressed E-selectin (data not shown). We have recently found using immunoelectron-microscopic method that SP is localized within specific granules of human skin MCs [31]. In addition to cutaneous sensory nerves, MC-derived SP may also affect the morphologic and immunologic alterations associated with the sebaceous glands and may contribute to the development of the inflammatory events in acne.…”

Section: Mast Cells In Acne Inflammationmentioning

confidence: 99%

New Aspects in Acne Inflammation

Toyoda

Morohashi²

2003

Dermatology

Self Cite

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There is ample clinical evidence suggesting that the nervous system such as emotional stress can influence the course of acne. We examined possible participation of cutaneous neurogenic factors including neuropeptides, neuropeptide-degrading enzymes and neurotrophic factors, in association with inflammation in the pathogenesis of acne. Immunohistochemical studies revealed that substance P (SP)-immunoreactive nerve fibers were in close apposition to the sebaceous glands, and that neutral endopeptidase (NEP) was expressed in the germinative cells of the sebaceous glands in the skin from acne patients. Nerve growth factor showed immunoreactivity only within the germinative cells. In addition, an increase in the number of mast cells and a strong expression of endothelial leukocyte adhesion molecule-1 on the postcapillary venules were observed in adjacent areas to the sebaceous glands. In vitro, the levels and the expression of stem cell factor by fibroblasts were upregulated by SP. When organ-cultured normal skin specimens were exposed to SP, we observed significant increases in the sizes of the sebaceous glands and in the number of sebum vacuoles in sebaceous cells. Furthermore, supplementation of SP to organ-cultured skin induced expression of NEP, and we demonstrated the subcellular localization of NEP in the endoplasmic reticulum and the Golgi apparatus within the sebaceous germinative cells using preembedding immunoelectron microscopy. These findings suggest that SP may stimulate lipogenesis of the sebaceous glands which may be followed by proliferation of Propionibacterium acnes, and may yield a potent influence on the sebaceous glands by provocation of inflammatory reactions via mast cells. Thus, cutaneous neurogenic factors should contribute to onset and/or exacerbation of acne inflammation.

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Immunolocalization of substance P in human skin mast cells

Cited by 55 publications

References 17 publications

Role of Substance P in an NC/Nga Mouse Model of Atopic Dermatitis-Like Disease

Role of Substance P in an NC/Nga Mouse Model of Atopic Dermatitis-Like Disease

Structure and Function of Smooth Muscle with Special Reference to Mast Cells

New Aspects in Acne Inflammation

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