Immunolocalization of cytoskeletal elements in human mammary epithelial cells

Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2

“…Taken together, our results and those of others suggest that dysregulation of Bcl-2 expression confers resistance to apoptosis induced by epithelial cell shape change. Although this does not directly induce tumor formation, it may contribute to the ability of tumor cells to tolerate the gross actin cytoskeletal abnormalities found in metastatic tumor cells (2,4,9,34).…”

“…Comparison of normal and malignant endometrial cells demonstrates a significantly reduced level of polymerized actin in the malignant cells (34). Disruption of actin organization also correlates with progression of tumor cells to adhesion-independent growth and increased invasiveness (2,9).…”

Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2

“…Taken together, our results and those of others suggest that dysregulation of Bcl-2 expression confers resistance to apoptosis induced by epithelial cell shape change. Although this does not directly induce tumor formation, it may contribute to the ability of tumor cells to tolerate the gross actin cytoskeletal abnormalities found in metastatic tumor cells (2,4,9,34).…”

“…Comparison of normal and malignant endometrial cells demonstrates a significantly reduced level of polymerized actin in the malignant cells (34). Disruption of actin organization also correlates with progression of tumor cells to adhesion-independent growth and increased invasiveness (2,9).…”

Quantitative analysis of cytokeratin network topology in the MCF7 cell line

“…The MCF7 human breast cancer cell line was widely investigated, especially for studying growth regulation. These cells maintained estrogen receptors and expressed cytokeratin but not vimentin as intermediate filaments (17,18). In MCF7, local intracytoplasmic variations in cytokeratin networks are observed (19), and different network patterns appear in specific intracytoplasmic loca-tions.…”

Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2

“…Taken together, our results and those of others suggest that dysregulation of Bcl-2 expression confers resistance to apoptosis induced by epithelial cell shape change. Although this does not directly induce tumor formation, it may contribute to the ability of tumor cells to tolerate the gross actin cytoskeletal abnormalities found in metastatic tumor cells (2,4,9,34).…”

“…Comparison of normal and malignant endometrial cells demonstrates a significantly reduced level of polymerized actin in the malignant cells (34). Disruption of actin organization also correlates with progression of tumor cells to adhesion-independent growth and increased invasiveness (2,9).…”

Quantitative analysis of cytokeratin network topology in the MCF7 cell line

“…The MCF7 human breast cancer cell line was widely investigated, especially for studying growth regulation. These cells maintained estrogen receptors and expressed cytokeratin but not vimentin as intermediate filaments (17,18). In MCF7, local intracytoplasmic variations in cytokeratin networks are observed (19), and different network patterns appear in specific intracytoplasmic loca-tions.…”

Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2

“…Taken together, our results and those of others suggest that dysregulation of Bcl-2 expression confers resistance to apoptosis induced by epithelial cell shape change. Although this does not directly induce tumor formation, it may contribute to the ability of tumor cells to tolerate the gross actin cytoskeletal abnormalities found in metastatic tumor cells (2,4,9,34).…”

“…Comparison of normal and malignant endometrial cells demonstrates a significantly reduced level of polymerized actin in the malignant cells (34). Disruption of actin organization also correlates with progression of tumor cells to adhesion-independent growth and increased invasiveness (2,9).…”

Quantitative analysis of cytokeratin network topology in the MCF7 cell line

“…The MCF7 human breast cancer cell line was widely investigated, especially for studying growth regulation. These cells maintained estrogen receptors and expressed cytokeratin but not vimentin as intermediate filaments (17,18). In MCF7, local intracytoplasmic variations in cytokeratin networks are observed (19), and different network patterns appear in specific intracytoplasmic loca-tions.…”