Immunohistopathological characterization of pig pneumonia caused by a combined Aujeszky's disease virus and Actinobacillus pleuropneumoniae infection

Narita, Minoru; Kawashima, Kenji; Nakagawa, H; Uchimura, A.; Ohashi, Takenobu; Kimura, Kumiko; Tanimura, Nobuhiko

doi:10.1016/s0021-9975(97)80064-3

Cited by 5 publications

(5 citation statements)

References 16 publications

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“…For example, the capillaries have endothelial gaps permitting exit of bacteria, and long loops lacking anastomoses; thus, thrombosis of the capillaries tends to produce small areas of necrosis, which are necessary for infections to become established. 13 Fibrinous thrombosis in the lung with immunohistochemical demonstration of A. pleuropneumoniae has been shown 8 and further hematogenous spread of the infection is possible, as bacteria have been isolated from the spleen of diseased pigs. 14 The chronicity and severity of the bone lesions made observation of the initial localization of the changes impossible in this case.…”

mentioning

confidence: 99%

Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent In Situ Hybridization

Jensen¹,

Boye²,

Hagedorn-Olsen³

et al. 1999

Vet Pathol

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Abstract. Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.

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mentioning

confidence: 99%

Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent In Situ Hybridization

Jensen¹,

Boye²,

Hagedorn-Olsen³

et al. 1999

Vet Pathol

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“…The synergistic effect of dual infection with PRRSV and other infectious agents has been investigated previously (Groschup et al, 1993;Kawashima et al, 1996;Reeth et al, 1996;Narita et al, 1997Narita et al, , 2000Thacker et al, 1999;De Bruin et al, 2000;Thanawongnuwech et al, 2000;Wills et al, 2000). In this study, pigs infected with PRRSV or PRV alone did not show severe clinical signs, but there was a febrile response in pigs infected with PRV, and there were slight respiratory symptoms in pigs infected with PRRSV.…”

Section: Discussionmentioning

confidence: 74%

“…Herpes viruses spread via sensory axons and infect sensory neurons in the ganglia of the peripheral nervous system (Cook and Stevens, 1973;Narita et al, 1991Narita et al, , 2001Chowdhury et al, 1997;Yanai et al, 2003). In PRV infection in pigs, infection spreads from the nasal cavity via the olfactory pathway to produce non-suppurative encephalitis (Narita et al, 1985(Narita et al, , 1991(Narita et al, , 1992(Narita et al, , 1993(Narita et al, , 1997. In the present experiments, one dually infected pig had severe encephalitis and three had encephalomalacia, characterized by neuronal degeneration with intranuclear inclusion bodies, a diffuse or focal glial reaction, and perivascular cuffing, as well as by cavitation accompanied by large numbers of macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Co-infection with PRRSV has been reported to increase the severity of disease produced by agents such as porcine respiratory coronavirus, influenza virus, mycobacteria, Salmonella choleraesuis and Streptococcus suis (Groschup et al, 1993;Kawashima et al, 1996;Reeth et al, 1996;Narita et al, 1997;Thacker et al, 1999;De Bruin et al, 2000;Thanawongnuwech et al, 2000;Wills et al, 2000). Such synergism has been supported by clinical, virological and immunological observations.…”

Section: Introductionmentioning

confidence: 87%

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Encephalomalacic Lesions in Pigs Dually Infected with Porcine Reproductive and Respiratory Syndrome Virus and Pseudorabies Virus

Narita

Ishii

2004

Journal of Comparative Pathology

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Four pigs (group 1) were infected with an aerosol containing porcine reproductive and respiratory syndrome virus (PRRSV) followed 7 days later by pseudorabies virus (PRV). Three further pigs (group 2) received PRRSV alone, two (group 3) received PRV alone, and two (group 4) remained as uninfected controls. Despite the admittedly small numbers of animals, the experiment appeared to throw light on aspects of synergy. Thus, the group 1 pigs showed severe neurological signs characterized by ataxia and muscular tremors. Total cell numbers in the bronchoalveolar lavage fluid were increased in all PRRSV-infected pigs, and PRRSV antigen was detected in the alveolar macrophages. Total cell numbers in the cerebrospinal fluid of group 1 pigs were considerably greater than those demonstrated in group 3, but no PRV antigen was found. Pigs of groups 1 and 2 showed pulmonary lesions, characterized by interstitial pneumonia and PRRSV antigen immunolabelling. Non-suppurative encephalitis was found in five of the six pigs of groups 1 and 3. In particular, one group 1 animal had severe necrotizing encephalitis with intranuclear inclusion bodies and associated immunolabelling of PRV antigen. The other three group 1 pigs had prominent malacic lesions, with macrophages. These neuropathological findings strongly suggested that PRRSV infection in pigs enhances the severity of brain lesions caused PRV.

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“…Sections were then counterstained in hematoxylin. The specificity of the staining procedures were ascertained by the introduction of negative controls consisting of experimentally Aujeszky's disease virus (ADV) -infected [12] and porcine reproductive and respiratory syndrome virus (PRRSV) -infected [8] porcine tissue samples. In addition, the primary antibodies raised to Nipah virus were substituted with the irrelevant monoclonal anti-canine distemper virus nucleoprotein antibody (VMRD, Inc., Pullman, WA) or anti-respiratory syncytial virus antibody (18B2) ( ARGENE Inc., Massapequa, NY).…”

Section: Nipah Virus Is a New Paramyxovirus Which Emerged Inmentioning

confidence: 99%

Reactivity of Anti-Nipah Virus Monoclonal Antibodies to Formalin-fixed, Paraffin-embedded Lung Tissues from Experimental Nipah and Hendra Virus Infections

Tanimura

Imada

Kashiwazaki³

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fix ed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virusinfected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for immunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis. KEY WORDS: immunohistochemistry, monoclonal antibody, Nipah virus.

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Immunohistopathological characterization of pig pneumonia caused by a combined Aujeszky's disease virus and Actinobacillus pleuropneumoniae infection

Cited by 5 publications

References 16 publications

Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent In Situ Hybridization

Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent In Situ Hybridization

Encephalomalacic Lesions in Pigs Dually Infected with Porcine Reproductive and Respiratory Syndrome Virus and Pseudorabies Virus

Reactivity of Anti-Nipah Virus Monoclonal Antibodies to Formalin-fixed, Paraffin-embedded Lung Tissues from Experimental Nipah and Hendra Virus Infections

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