Immunohistological study of small Rho GTPases and β-catenin during regeneration of the rat submandibular gland

Shiratsuchi, Hiroshi; Shimizu, Osamu; Saito, Tadahito; Mashimo, Takayuki; Yonehara, Yoshiyuki

doi:10.1007/s10735-012-9437-8

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…Histologically, atrophy and disappearance of acinar cells, and gradual increases in duct-like structures and fibrous connective tissue were observed. Changes in salivary gland tissue after short-term ligation that have been reported include enlargement of duct lumens, atrophy and disappearance of acinar cells above the ligation, increases in ductlike structures and proliferation of fibrous connective tissue, degranulation and vacuolar degeneration of granular duct cells and acinar cells, and disappearance of basal striations from the striated duct [8][9][10][11][12][13][14][15][16][17][18] . This was similar to findings in the long-term ligation.…”

Section: Discussionmentioning

confidence: 99%

“…Histological studies of atrophied salivary glands have reported the occurrence of duct-like structures 17,18,[29][30][31] . Duct-like structures resulting from ligation were also observed in the present study, and it was shown that these increase over time with long-term ligation.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in salivary gland tissue that have been found as a result of duct ligation include atrophy and disappearance of acinar cells above the ligation, an increase in duct-like structures, and an increase in fibrous connective tissue [8][9][10][11][12][13][14][15][16][17][18] . There are also reports noting residual salivary gland function, restorative function, and regenerative ability in atrophied salivary glands [19][20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

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Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

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To examine the pathology of salivary glands that have undergone atrophy or hypofunction due to old age or disease, the duct ligation model is used. This model has been used in studies on the course of glandular parenchyma atrophy and the potential for repair and regeneration. However, the period of ligation was short in most of these studies, and none have examined long-term progress. Therefore, we investigated long-term ligation of the submandibular gland in mice and examined the chronic changes in atrophied salivary glands. The ligation periods were 1, 2 and 3 months, and atrophied salivary glands were resected after each period. Resected glands were examined by histology, immunohistochemistry, RT-PCR, and transmission electron microscopy. Histologically, disappearance of acinar cells and increases in duct-like structures were observed over time in atrophied salivary glands, resulting from long-term submandibular gland main excretory duct ligation. Acinar cell markers (-amylase, aquaporin 5) showed marked weakness in expression after ligation, but expression was still observed after 3 months of ligation. Stem cell markers (c-kit, Sca-1) showed greater expression at 1 month of ligation, compared with controls, but expression subsequently decreased with time. Expression of the precursor cell marker cytokeratin 5 was retained throughout long-term ligation. Atrophied salivary gland tissue resulting from long-term ligation showed increases in specific duct-like structures over time, and the expression of stem cell markers and progenitor cell markers in the area of these structures suggests that the repair capability remained intact.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

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“…Several studies have investigated tissue changes in the glandular parenchyma as a result of ligation of the main excretory duct of the submandibular gland; all have reported acinar cell atrophy, increase in interlobular connective tissue, and enlargement of the lumina as histological changes [6][7][8][9][10][11][12][13] . Here we investigated the expression of the water channel protein AQP5, a marker for acinar cells [14][15][16] , in mice and of the secretase α-amylase 17,18) after different periods of ligation to evaluate the function of residual acinar cells.…”

Section: Discussionmentioning

confidence: 99%

Regenerative Capacity of Atrophic Submandibular Gland by Duct Ligation in Mice

Akadomari

Tanaka

Mataga

2016

J. Hard Tissue Biology.

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Function degeneracy of the salivary gland leads to reduced protection against infection and reduced physiological function of the oral cavity, considerably diminishing the patient's quality of life. However, no method of treatment has yet been established to recover the secretory capacity of the salivary gland reduced by the degeneration or disappearance of the gland tissue. Ongoing salivary gland regeneration experiments using cell transplantation aim to introduce clinical regenerative treatment for the salivary gland. Because it is essential to understand the self-regenerative capacity that remains in the recipient's salivary gland, it is essential to search for optimal conditions for transplantation and establish a salivary gland atrophy model. In this context, we performed duct ligation of the submandibular gland in groups of mice for 7-, 14-, and 21-day ligation periods, compared immunohistochemical staining using acinar cell and stem cell markers, detected apoptosis using TUNEL staining, and analyzed gene expression using RT-PCR to search for the expression of factors related to regenerative capacity that remain in the atrophic salivary gland. All 3 periods of ligation led to remarkable atrophy/disappearance of acinar cells and the dominant presence of duct-like structures. These structures expressed stem cell markers most intensively after 14 days of ligation, and double immunostaining revealed the expressions of PSCA and c-Kit that coincided with them. AQP5 expression was detected in acinar cells and the apical membrane of the intercalated ducts in the normal submandibular gland and was also detected after 21 days of ligation in the remnant acinar cells and intercalated ducts. Conversely, the number of TUNELpositive apoptotic cells in the atrophic salivary gland was highest after 7 days of ligation. These results showed that the duct-like structures that exist after ligation expressed stem cell markers and AQP5, indicating the presence of a mechanism related to the regeneration of salivary gland tissue.

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“…Salivary gland tissue can be damaged by conditions such as sialadenitis, sialolithiasis, trauma, and radiation therapy, which can lead to degeneration, atrophy, and necrosis. Methods to examine these pathological conditions include surgical resection [1][2][3], duct ligation [4][5][6], radiation [7,8], laser irradiation [9,10], and histopathological and immunohistochemical analysis.…”

Section: Introductionmentioning

confidence: 99%

Wound healing after applying a gelatin-based hydrogel sheet to resected rat submandibular gland

et al. 2020

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The present study was done to develop a useful experimental model for analysis of the effects of physiologically active substances on atrophy and regeneration of salivary gland acinar cells. Resection wounds (diameter, 3 mm) were made in the submandibular glands of 8-week-old Wistar rats (n = 24) for histochemical examination on Days 3, 5, 7, 10, 14, and 21 after implantation of a gelatin-based hydrogel sheet. The results showed that the sheet had nearly disappeared by Day 10. Regions around the resection wounds were classified as normal, atrophic, or necrotic. In atrophic regions, acinar cells atrophied after resection, and few acinar cells were observed on Day 7. On Days 5-7, striated and granular ducts resembled duct-like structures. On Day 10, newly formed acinar cells were confirmed by increased periodic acid-Schiff staining, and a greater number of mature cells was present thereafter. In necrotic regions, acinar and ductal cells were destroyed, and scattered enucleated acinar cells and ductlike structures were present, on Day 3; newly formed acinar cells were observed on Day 10. Thus, the experimental model demonstrated atrophy and regeneration of the submandibular gland and enabled analysis of the effects of sustained release of physiologically active substances contained within an implanted sheet.

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Immunohistological study of small Rho GTPases and β-catenin during regeneration of the rat submandibular gland

Cited by 6 publications

References 37 publications

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Regenerative Capacity of Atrophic Submandibular Gland by Duct Ligation in Mice

Wound healing after applying a gelatin-based hydrogel sheet to resected rat submandibular gland

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