Immunohistochemical location of the p75 neurotrophin receptor (p75NTR) in oral leukoplakia and oral squamous cell carcinoma

Kiyosue, Takahiro; Kawano, S; Matsubara, Ryota; Goto, Yuichi; Hirano, Mitsuhiro; Jinno, Teppei; Toyoshima, Takeshi; Kitamura, Ryoji; Oobu, Kazunari; Nakamura, Seiji

doi:10.1007/s10147-011-0358-4

Cited by 39 publications

(33 citation statements)

References 42 publications

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“…71,72,73,74 One study showed that the labeling index of p75 was similar among normal and oral leukoplakia samples, whereas increased p75 expression correlated with a worse OSCC tumor grade. 75 Another group showed that K15 was down-regulated in oral lichen planus, while α6-integrin, β1-integrin and melanoma chondroitin sulphate proteoglycan were upregulated in both oral lichen planus and hyperkeratotic oral lesions. 76 Others also reported that oral dysplasias had decreased expression of p63 (ref.…”

Section: Stem Cells In Oral Diseasementioning

confidence: 99%

“…Additionally, several studies have attempted to correlate putative CSC markers with patient prognosis and survival, showing that tumors expressing higher levels of certain CSC markers tend to have poorer outcomes. 72,75,78,79,80,81 One caveat is that unless serial transplantation assays have shown that the stem cell markers being studied actually label CSCs, it is difficult to draw firm conclusions about the identity and function of positively staining cells within OSCCs. This is true even for stem cell markers that have already been shown to label normal OESCs, since these cells may not perform similar functions within tumors.…”

Section: Stem Cells In Oral Diseasementioning

confidence: 99%

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Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Jones

Klein

2013

Int J Oral Sci

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The identification and characterization of stem cells is a major focus of developmental biology and regenerative medicine. The advent of genetic inducible fate mapping techniques has made it possible to precisely label specific cell populations and to follow their progeny over time. When combined with advanced mathematical and statistical methods, stem cell division dynamics can be studied in new and exciting ways. Despite advances in a number of tissues, relatively little attention has been paid to stem cells in the oral epithelium. This review will focus on current knowledge about adult oral epithelial stem cells, paradigms in other epithelial stem cell systems that could facilitate new discoveries in this area and the potential roles of epithelial stem cells in oral disease.

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Section: Stem Cells In Oral Diseasementioning

confidence: 99%

Section: Stem Cells In Oral Diseasementioning

confidence: 99%

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Jones

Klein

2013

Int J Oral Sci

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“…According to the results of a scratch assay, p75 NTR+ cells possess migratory ability. Kiyosue et al (12) reported a significant association between the magnitude of p75 NTR positivity and the mode of tumor invasion. Furthermore, Soland et al (46) reported that the average risk of local recurrence was increased by ~17-fold in OSCC when p75 NTR expression was positive, and marked tumor cell dissociation was detected at the invasive front.…”

Section: Discussionmentioning

confidence: 99%

“…It has previously been reported that the p75 neurotrophin receptor (p75 NTR ) may be involved in the invasion and poor prognosis of OSCC (12). As a member of the tumor necrosis factor superfamily, p75 NTR is a 75-kDa cell-surface receptor glycoprotein (13,14), which is involved in diverse cellular responses, including cell proliferation and survival, and apoptosis in neural and non-neural tissues (15,16) via unique pathways (17,18) or activation of the intrinsic caspase pathway (19).…”

Section: Introductionmentioning

confidence: 99%

p75 neurotrophin receptor: A potential surface marker of tongue squamous cell carcinoma stem cells

Tong

Sun

Huang

et al. 2017

Molecular Medicine Reports

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The present study detected p75 neurotrophin receptor (p75NTR) expression in tongue squamous cell carcinoma (TSCC) cell lines, in order to define the biological properties of p75NTR+ cells and to confirm the use of p75NTR+ as a surface marker for TSCC stem cells. p75NTR+ cells were separated from Tca-8113 and CAL-27 TSCC cells by fluorescence-activated cell sorting. Colony formation, MTT and scratch assays, and a tumorigenicity analysis were performed to measure self-renewal and proliferation, multidirectional differentiation, and tumorigenicity of p75NTR+ cells. p75NTR+ cells comprised 3.1 and 1.9% of Tca-8113 and CAL-27 cells (mean of three experiments), respectively, and were more able to form colonies compared with non-sorted cells (P<0.01). In addition, the proportion of p75NTR+ cells generated from monoclonal p75NTR+ cells decreased to 14.5 (Tca-8113) and 5.8% (CAL-27) of cells within 2 weeks, thus suggesting that p75NTR+ cells are able to generate p75NTR+ and p75NTR− cells. Furthermore, p75NTR+ cells exhibited increased proliferation, as evidenced by MTT assay (P<0.01) and had greater metastatic ability according to the scratch assay (P<0.01), compared with non-sorted cells. p75NTR+ cells also exhibited a greater tumorigenic capacity compared with non-sorted cells. In conclusion, p75NTR+ cells isolated from TSCC cell lines possess the characteristics of cancer stem cells; therefore, p75NTR may be considered a useful surface marker for the identification of TSCC stem cells.

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“…TrkA is expressed in basal and parabasal mucosal and gingival epithelial cells, with weaker expression in spinous and granular cell layers, while p75 neurotrophin receptor (p75 NTR ) only occurs in basal cell layers (Figure 5) [14]. p75 NTR is also expressed in oral squamous cell carcinomas (OSCCs) [16,17] and can be used as a prognostic marker together with the pattern of invasion. We found that high expression of p75 NTR in the central, superficial and invasive parts of the tumor was a marker for recurrence.…”

Section: Expression Of Ngf Receptors In the Oral Cavity And The Samentioning

confidence: 99%

The Role of Nerve Growth Factor (NGF) and Its Precursor Forms in Oral Wound Healing

Schenck

Schreurs

Hayashi

et al. 2017

IJMS

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Nerve growth factor (NGF) and its different precursor forms are secreted into human saliva by salivary glands and are also produced by an array of cells in the tissues of the oral cavity. The major forms of NGF in human saliva are forms of pro-nerve growth factor (pro-NGF) and not mature NGF. The NGF receptors tropomyosin-related kinase A (TrkA) and p75 neurotrophin receptor (p75NTR) are widely expressed on cells in the soft tissues of the human oral cavity, including keratinocytes, endothelial cells, fibroblasts and leukocytes, and in ductal and acinar cells of all types of salivary glands. In vitro models show that NGF can contribute at most stages in the oral wound healing process: restitution, cell survival, apoptosis, cellular proliferation, inflammation, angiogenesis and tissue remodeling. NGF may therefore take part in the effective wound healing in the oral cavity that occurs with little scarring. As pro-NGF forms appear to be the major form of NGF in human saliva, efforts should be made to study its function, specifically in the process of wound healing. In addition, animal and clinical studies should be initiated to examine if topical application of pro-NGF or NGF can be a therapy for chronic oral ulcerations and wounds.

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Immunohistochemical location of the p75 neurotrophin receptor (p75NTR) in oral leukoplakia and oral squamous cell carcinoma

Abstract: These results suggest that p75NTR is expressed in undifferentiated cell populations in OL and OSCC. Furthermore, p75NTR is possibly involved in invasion and poor prognosis in OSCC.

Cited by 39 publications

References 42 publications

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

p75 neurotrophin receptor: A potential surface marker of tongue squamous cell carcinoma stem cells

The Role of Nerve Growth Factor (NGF) and Its Precursor Forms in Oral Wound Healing

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