Immunohistochemical localization and dynamics of paraquat in the stomach and esophagus of rats

Nagao, Masataka; Zhang, Weidong; Itakura, Yoshiyuki; Kobayashi, Masahiko; Yamada, Yoshihiro; Yagi, Katsunori; Oono, T; Takatori, Takehiko

doi:10.1007/bf01225235

Cited by 7 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the dynamics of PQ in rats has been rarely investigated. Studies[ 38 , 39 ] revealed that plasma paraquat concentration of rats maintained high within 24 hours after PQ poisoning and vanished within 72 hours, which can be detected in the lungs, stomach and intestine 10 days after administration of PQ.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of smecta or smectite powder on rats with paraquat toxication

Jiang¹,

Ma²,

Wang³

et al. 2013

World Journal of Emergency Medicine

View full text Add to dashboard Cite

BACKGROUND:The plasma concentration of paraquat is closely related to the prognosis of patients with paraquat toxication, and the most common cause of death from paraquat poisoning is multiple organ failure (MOF). This study aimed to evaluate therapeutic effect of smecta on the plasma concentrations of paraquat and multi-organ injury induced by paraquat intoxication in rats.METHODS:A total of 76 healthy adult SD rats were randomly divided into group A (control group, n=6), group B (poisoned group, n=30) and group C (smecta-treated group, n=30). Rats in groups B and C were treated intragastrically with PQ at 50 mg/kg, and rats in group A was treated intragastrically with saline (1 mL). Rats in group C were given intragastrically smecta at 400 mg/kg 10 minutes after administration of PQ, while rats in other two groups were treated intragastrically with 1 mL saline at the same time. Live rats in groups B and C were sacrificed at 2, 6, 24, 48, 72 hours after administration of PQ for the determination of paraquat plasma concentrations and for HE staining of the lung, stomach and jejunum. The rats were executed at the end of trial by the same way in group A.RESULTS:The plasma concentration of paraquat (ng/mL) ranged from 440.314±49.776 to 4320.6150±413.947. Distinctive pathological changes were seen in the lung, stomach and jejunum in group B. Lung injuries deteriorated gradually, edema, leukocyte infiltration, pneumorrhagia, incrassated septa and lung consolidation were observed. Abruption of mucosa, hyperemic gastric mucosa and leukocyte infiltration were obvious in the stomach. The hemorrhage of jejunum mucosa, the abruption of villus, the gland damage with the addition of inflammatory cell infiltration were found. Compared to group B, the plasma concentration of paraquat reduced (P<0.01) and the pathological changes mentioned above were obviously alleviated in group C (P<0.05, P<0.01).CONCLUSION:Smecta reduced the plasma concentration of paraquat and alleviated pathologic injury of rats with PQ poisoning.

show abstract

Section: Discussionmentioning

confidence: 99%