Immunohistochemical expression of dogTERT in canine testicular tumours in relation to PCNA, ki67 and p53 expression.

Papaioannou, Nikolaos; Psalla, Dimitra; Zavlaris, Michael; Loukopoulos, Panayiotis; Τziris, N.; Vlemmas, I.

doi:10.1007/s11259-009-9308-2

Cited by 13 publications

(7 citation statements)

References 53 publications

(91 reference statements)

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“…In dogs, PCNA was recognized as a useful proliferation marker being associated with recurrence after surgical treatment in mammary tumours and in mast cell tumours . Meanwhile, other authors reported no correlation with prognosis, recurrence or metastasis in testicular as well as in mammary tumours . To the authors' knowledge, PCNA's prognostic value has never been evaluated in canine OSCC.…”

Section: Discussionmentioning

confidence: 93%

PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis

Mestrinho¹,

Faísca

Peleteiro³

et al. 2014

Vet Comparative Oncology

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This study evaluated the prognosis factors of age, tumour size, anatomic location, histological grade and proliferating cell nuclear antigen (PCNA) expression in 13 dogs with oral squamous cell carcinoma (OSCC) with bone invasion and without signs of lymph node or distant metastasis. All animals were treated with radical excision performed with at least 1 cm margin, based on computed tomography images. In the 2-year follow-up, median disease-free survival was 138 days for dogs with grade 3 tumours and was not reached for those with grade 2 tumours. Grade 3 tumours and PCNA labelling index ≥65% were related with a shorter disease-free survival time and consequently poor prognosis (p = 0.003 and p = 0.034, respectively). Mean PCNA labelling index was significantly higher in recurrent cases (p = 0.011). Histological grade and PCNA expression may be important prognosis factors in canine OSCC.

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Section: Discussionmentioning

confidence: 93%

PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis

Mestrinho¹,

Faísca

Peleteiro³

et al. 2014

Vet Comparative Oncology

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“…Both canine and male seminomas showed the strongest expression of Ki-67 in all testicular tumors analyzed in our study, however, with only slightly weaker immunoreactivity in Leydig cell tumors. Also Papaioannou et al showed that the nuclear expression of Ki-67 is the strongest in seminomas, and somehow weaker in other types of canine testicular tumors [43]. The usefulness of Ki-67 antigen as a marker of cell proliferation was also confirmed in human testicular germ cell tumors [44].…”

Section: Discussionmentioning

confidence: 99%

Inhibin-α, E-cadherin, calretinin and Ki-67 antigen in the immunohistochemical evaluation of canine and human testicular neoplasms

Ciaputa

Nowak

Madej

et al. 2015

Folia Histochem Cytobiol.

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Introduction. The steady increase of dogs with diagnosed testicular neoplasms observed in recent years prompted us to carry out immunohistochemical (IHC) studies for their better characterization. The aim of the study was to analyze most common canine testicular neoplasms (seminomas, Leydig cell and Sertoli cell tumors) with selected IHC markers and to compare the expressions of these proteins in corresponding canine and human testicular tumors. Material and methods. Studies were carried out on testicular canine tumors: 40 cases of seminoma, 40 cases of Leydig cell tumor and 40 cases of Sertoli cell tumor. Moreover, 15 cases of human seminomas and 5 cases of human Leydig cell tumors were also analyzed. Immunohistochemistry was performed on paraffin sections by standard technique using monoclonal anti-human antibodies against E-cadherin, inhibin-a, calretinin and Ki-67. The slides were subjected to computer-aided image analysis and the intensity of the immunoreactivity was assessed by a semi-quantitative scoring system. Results. Due to the very low prevalence of the Sertoli cell-derived tumors in the human population, we were able to examine the markers' expression only in the canine gonadal tumors. We revealed that, apart from E-cadherin in Leydig cell tumors and calretinin in seminomas, the expression of all the analyzed markers in canine and human testicular tumors was similar. E.g. there was no immunoexpression of inhibin-a in 75% of canine and 100% of human cases of seminoma. The immunoreactivity of Ki-67 was intense in 40% of canine and 60% of human seminomas. Immunoexpression of inhibin-a in Leydig cell tumor was intense in 70% of canine and 100% of human cases, respectively. Also the immunoreactivity of calretinin was intense in 75% of cases of canine and 100% of human Leydig cell tumors. In 50% of canine and 40% of human Leydig cell tumors, the immunoexpression of Ki-67 was weak.Conclusions. The applied anti-human monoclonal antibodies against common antigens and markers of human testicular neoplasms could be routinely used for the immunohistochemical evaluation of canine testicular tumors.

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“…Samples from the duodenum, the jejunum, the ileum and the cecum were evaluated for cell proliferation according to the method described by Papaioannou et al (2009) . Fixed tissues were embedded in paraffin wax and serial sections (4–6 µ m) were cut.…”

Section: Methodsmentioning

confidence: 99%

Effects of Oregano, Attapulgite, Benzoic Acid and their Blend on Chicken Performance, Intestinal Microbiology and Intestinal Morphology

et al. 2017

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The aim of the present study was to investigate the effects of oregano, attapulgite, benzoic acid and their combination on broiler performance, microflora composition of jejunum and cecum, intestinal architecture and breast and thigh meat composition. A total of 400 one-day-old broiler chicks were used in a 42-day trial. They were randomly distributed into five treatments with four replicates of twenty chickens per pen: Control group; Attapulgite group; Oregano essential oil group; Benzoic acid group; Mixed group. At the end of the trial, total counts of bacteria, Enterobacteriaceae, Lactobacilli, and Clostridium perfringens were enumerated by real time PCR at both jejunum and cecum. Intestinal morphology was carried out in duodenum, jejunum and ileum, for villus height and crypt depth. Cell proliferation was also evaluated in the small intestine and the cecum. The results showed that oregano and benzoic acid improved some growth performance parameters. The combined use of the examined substances increased enterobacteria counts in the jejunum, and cell proliferation in the duodenum and the jejunum. Benzoic acid improved intestinal wall morphology in the ileum. In conclusion, the combined dietary supplementation with oregano, attapulgite and benzoic acid can be a novel tool to beneficially modulate broiler chickens performance.

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Immunohistochemical expression of dogTERT in canine testicular tumours in relation to PCNA, ki67 and p53 expression.

Cited by 13 publications

References 53 publications

PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis

PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis

Inhibin-α, E-cadherin, calretinin and Ki-67 antigen in the immunohistochemical evaluation of canine and human testicular neoplasms

Effects of Oregano, Attapulgite, Benzoic Acid and their Blend on Chicken Performance, Intestinal Microbiology and Intestinal Morphology

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