Immunohistochemical Demonstration of Keratin 19 Expression in Isolated Human Hair Follicles

Narisawa, Yutaka; Hashimoto, Ken; Kohda, Hiromu

doi:10.1111/1523-1747.ep12392730

Cited by 42 publications

(25 citation statements)

References 25 publications

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“…As previously demonstrated in facial vellus hairs, in the present study we show that the AP muscle surrounds the entire follicular structure of scalp terminal follicles. In 1994, it was demonstrated that follicular stem cells (K19.1‐reactive cells) are present either continuously or at intervals in the outermost cell layer of the outer root sheath of terminal hair follicles of the scalp 6 . Assuming that the unique interactions between the bulge and the AP muscle could play an important role in maintaining the phenotype of the bulge cells, 7 this variant of proximal insertion could be related to the presence of K19.1‐reactive cells around the entire outer root sheath of terminal hair follicles of the scalp.…”

Section: Discussionmentioning

confidence: 99%

Arrector pili muscle: evidence of proximal attachment variant in terminal follicles of the scalp

2002

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Section: Discussionmentioning

confidence: 99%

Arrector pili muscle: evidence of proximal attachment variant in terminal follicles of the scalp

2002

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“…The corresponding subpopulations have been identified and characterized by specific histological and immunological markers [3][4][5][6][7][8][9][10][11][12][13][14][15], and also by the formation of boundaries and communication compartments [35]. With respect to the ORS, both functional [19,20] and immunocytochemical [9][10][11] data had indicated some heterogeneity in this compartment, suggesting that distinct differentiation programmes occurred in the U-ORS and L-ORS [6].…”

Section: Discussionmentioning

confidence: 99%

The distribution of ? 2 ? 1, ? 3 ? 1 and ? 6 ? 4 integrins identifies distinct subpopulations of basal keratinocytes in the outer root sheath of the human anagen hair follicle

Commo

Bernard

1997

Cellular and Molecular Life Sciences (CMLS)

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The human hair follicle is composed of different concentric compartments, which reflect different programmes of differentiation. Using monoclonal antibodies against alpha 2 beta 1 and alpha 3 beta 1 integrins we demonstrated a shift in their expression, from a basolateral distribution in the basal cells of the lower outer root sheath, to an apicolateral expression in the upper outer root sheath, as in epidermis. This shift takes place in a transition zone, localized to the midpart of the follicle. The distinct basolateral distribution of alpha 2 beta 1 and alpha 3 beta 1 integrins in the lower portion of the outer root sheath coincides with the presence of basal cell protrusions and is probably linked to the presence of the vitreous membrane which surrounds the bottom part of the anagen human hair follicle. Moreover, we showed that the expression of alpha 6 beta 4 integrin is discontinuous along the hair follicle and coincides with that of laminin 5. Together these results establish that within a given compartment-namely the outer root sheath-several domains can be clearly identified, which probably reflect the onset of successive differentiation pathways along the hair follicle.

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“…The arrector pili muscle bundle branched into this protuberance. Since the bulge area is the attachment site of the arrector pili muscle and is characterized by the presence of CAM5.2-positive Merkel cells [8] and Ks19.1 immunoreactivity [12], the newly formed protuberance is the area of the secondary hair germ equivalent to the bulge in early anagen hair follicles.…”

Section: Discussionmentioning

confidence: 99%

“…8 (52.5 kDa (Becton-Dickinson, San Jose, Calif., USA) [10], such as that found in Merkel cells, and monoclonal murine antibody Ks19.1 specific for cytokeratin no. 19 (40 kDa) (ICN Immunologicals, Lisle, Ill.) [11], which are suitable markers for the bulge area [12]. The sheets were then washed with PBS three times for 10 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Two- and three-dimensional demonstrations of morphological alterations of early anagen hair follicle with special reference to the bulge area

Narisawa

Kohda

1996

Arch Dermatol Res

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During late telogen to early anagen secondary hair germ is newly formed by the downgrowth of a clubbed column which is indistinguishable from the bulge. Serial vertical sections demonstrated that the early anagen terminal hair follicle formed the new secondary hair germ associated with a lateral protuberance of basaloid cells which could be considered as the bulge of the new hair follicle. Interestingly, the arrector pili muscle bundle was divided into two branches, one inserted into the original clubbed end and the other into this protuberance of the secondary hair germ. CAM5.2-reactive Merkel cells were present not only in the clubbed ends of the old follicle but also in the protuberance of the new hair germ. The formation of the lateral protuberance of the new hair germ preceded the appearance of CAM5.2-reactive Merkel cells in this location. Ks19.1 immunoreactivity was observed from the clubbed end to the upper half of the new hair germ. These phenomena occurred in early anagen before the club hairs were shed. It is postulated that the early anagen hair follicle formed the area within the new hair germ equivalent to the bulge and Merkel cells either moved from the bulge of the old hair follicle or differentiated de novo from immature epithelial cells. Merkel cells or their products in the bulge may serve as attractants for the readjusting arrector pili muscle to anchor to the bulge of the new hair follicle.

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Immunohistochemical Demonstration of Keratin 19 Expression in Isolated Human Hair Follicles

Cited by 42 publications

References 25 publications

Arrector pili muscle: evidence of proximal attachment variant in terminal follicles of the scalp

Arrector pili muscle: evidence of proximal attachment variant in terminal follicles of the scalp

The distribution of ? 2 ? 1, ? 3 ? 1 and ? 6 ? 4 integrins identifies distinct subpopulations of basal keratinocytes in the outer root sheath of the human anagen hair follicle

Two- and three-dimensional demonstrations of morphological alterations of early anagen hair follicle with special reference to the bulge area

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