Immunohistochemical and Polymerase Chain Reaction Study on Expression of HPV and HSV and Its Histopathological Pattern Correlation in Cervical Lesions

Abstract

BACKGROUNDCervical cancer has a major impact on developing countries, where screening programmes are not well established or effective. AIMThis study aims to detect HPV and HSV in cervical lesions using Immunohistochemistry and Polymerase chain reaction. SETTINGS AND DESIGN85 biopsy samples were obtained from out and inpatients attending Gynaecology Department in Gandhi Medical College and associated hospital, Bhopal between January -August 2015. MATERIALS AND METHODSTwo biopsy specimens of the cervix were simultaneously collected from the same patient; one for histopathological examination and other for polymerase chain reaction. The samples were graded as chronic cervicitis, Cervical Intraepithelial Neoplasia (CIN) I, II, III, and Squamous Cell Carcinoma (SCC) and cervical adenocarcinoma by pathologist in Department of Pathology. RESULTSOut of 85 cervical biopsies, HPV (IHC) positivity was maximum for chronic cervicitis with dysplasia (77.8%). IHC using HSV in cervical lesions showed 45.2% positivity in chronic cervicitis; 44.4% in chronic cervicitis with dysplasia and 21.9% in squamous cell carcinoma. CONCLUSIONThis study reveals a significant detection of HPV in the individuals with pre-cancerous lesions by the use of advanced techniques such as IHC and PCR.

Genital Hygiene and Strategies for HPV Prevention

“…Till date, enough research has not been done to determine the direct link between HPV and personal genital hygiene. Genital hygiene play a role in the decreasing the chances of yeast infections, bacterial vaginosis and HPV [6]. There is a lack of consensus of HPV infection with respect to reproductive and genital health.…”