Immunohistochemical analysis of pRb2/p130, VEGF, EZH2, p53, p16^INK4A, p27^KIP1, p21^WAF1, Ki‐67 expression patterns in gastric cancer

Abstract

Although the considerable progress against gastric cancer, it remains a complex lethal disease defined by peculiar histological and molecular features. The purpose of the present study was to investigate pRb2/p130, VEGF, EZH2, p53, p16INK4A, p27KIP1, p21WAF1, Ki‐67 expressions, and analyze their possible correlations with clinicopathological factors. The expression patterns were examined by immunohistochemistry in 47 patients, 27 evaluated of intestinal‐type, and 20 of diffuse‐type, with a mean follow up of 56 months and by Western blot in AGS, N87, KATO‐III, and YCC‐2, ‐3, ‐16 gastric cell lines. Overall, stomach cancer showed EZH2 correlated with high levels of p53, Ki‐67, and cytoplasmic pRb2/p130 (P < 0.05, and P < 0.01, respectively). Increased expression of EZH2 was found in the intestinal‐type and correlated with the risk of distant metastasis (P < 0.05 and P < 0.01, respectively), demonstrating that this protein may have a prognostic value in this type of cancer. Interestingly, a strong inverse correlation was observed between p27KIP1 expression levels and the risk of advanced disease and metastasis (P < 0.05), and a positive correlation between the expression levels of p21WAF1 and low‐grade (G1) gastric tumors (P < 0.05), confirming the traditionally accepted role for these tumor‐suppressor genes in gastric cancer. Finally, a direct correlation was found between the expression levels of nuclear pRb2/p130 and low‐grade (G1) gastric tumors that was statistically significant (P < 0.05). Altogether, these data may help shed some additional light on the pathogenetic mechanisms related to the two main gastric cancer histotypes and their invasive potentials. J. Cell. Physiol. 210: 183–191, 2007. © 2006 Wiley‐Liss, Inc.

Enhancer of Zeste Homologue 2 Expression in Breast Carcinoma Smears in Relationship with p53, Ki-67 and Other Prognostic Parameters

“…In the present study, 64% of the cases expressed high levels of EZH 2, and this finding is in good agreement with reports by other teams [27,28]. Additionally, EZH 2 expression correlated with higher levels of p53 (46%) and Ki-67 (48%).…”

“…The search for molecular factors highly correlated with prognosis may lead to the discovery of factors that can help to predict patient survival [1,2]. Recently, EZH 2 has been found to be involved in cell cycle regulation, and a significant association has been demonstrated between EZH 2 expression and tumor cell proliferation as well as features of aggressive tumor behavior and reduced survival [27,28,29]. …”

CIP2A Is Overexpressed in Gastric Cancer and Its Depletion Leads to Impaired Clonogenicity, Senescence, or Differentiation of Tumor Cells

“…3C, there were no clear increases in p53 and p21 in all the cells treated with CIP2A siRNA compared with the control cells. In addition, to corroborate the above results, we determined the phosphorylation of p53 at Ser 15 , a marker for p53 activation, using its specific antibody. The phosphorylated p53 signal at this residual was undetectable in both AGS and HCT116p53+/+ cells (data not shown), in good accordance with unchanged total p53 levels.…”

Study on the Different Expression of Molecular Markers between Cardiac Cancer and Distal Gastric Cancer and Their Correlations with Clinicopathological Features

“…Some studies on the expression of molecular markers in gastric cancer revealed that p21 expression was positively correlated with the expression level of p27 [21]. It has been postulated that the growth-inhibitory activity of p27 and p21 may be modulated in advanced tumors through inactivation [22,23].…”