Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Mohit, Behzad

doi:10.1073/pnas.68.12.3045

Cited by 19 publications

(3 citation statements)

References 17 publications

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“…5 In patients in whom both intact immunoglobulin and light chain are secreted, it is assumed that they originate in different MM plasma cell clones with different levels of differentiation. 6 In fact, in the course of MM progression especially with multiple relapses, dedifferentiation is frequently observed, characterized by "Bence Jones escape," cessation of M-protein secretion, or "high-grade transformation" (best captured by increased levels of serum lactate dehydrogenase [LDH]). 7,8 The elusive MM stem cell is thought to have a pre-plasma cell phenotype incapable of immunoglobulin secretion, 9 although others have shown that the terminally differentiated plasma cell retained self-renewal potential, 10 consistent with the recent notion of stem cell plasticity.…”

Section: Introductionmentioning

confidence: 99%

High serum-free light chain levels and their rapid reduction in response to therapy define an aggressive multiple myeloma subtype with poor prognosis

Rhee

Bolejack

Hollmig

et al. 2007

Blood

168

103

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Serum-free light chain (SFLC) levels are useful for diagnosing nonsecretory myeloma and monitoring response in lightchain-only disease, especially in the presence of renal failure. As part of a tandem autotransplantation trial for newly diagnosed multiple myeloma, SFLC levels were measured at baseline, within 7 days of starting the first cycle, and before both the second induction cycle and the first transplantation. SFLC baseline levels higher than 75 mg/dL (top tertile) identified 33% of 301 patients with higher near-complete response rate (n-CR) to induction therapy (37% vs 20%, P ‫؍‬ .002) yet inferior 24-month overall survival (OS: 76% vs 91%, P < .001) and event-free survival (EFS: 73% vs 90%, P < .001), retaining independent prognostic significance for both EFS (HR ‫؍‬ 2.40, P ‫؍‬ .008) and OS (HR ‫؍‬ 2.43, P ‫؍‬ .016). Baseline SFLC higher than 75 mg/dL was associated with light-chain-only secretion (P < .001), creatinine level 176.8 M (2 mg/dL) or higher (P < .001), beta-2-microglobulin 297.5 nM/L (3.5 mg/L) or higher (P < .001), lactate dehydrogenase 190 U/L or higher (P < .001), and bone marrow plasmacytosis higher than 30% (P ‫؍‬ .003). Additional independent ad-

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Section: Introductionmentioning

confidence: 99%

High serum-free light chain levels and their rapid reduction in response to therapy define an aggressive multiple myeloma subtype with poor prognosis

Rhee

Bolejack

Hollmig

et al. 2007

Blood

168

103

View full text Add to dashboard Cite

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“…Fusion of mouse hepatoma cells with human leukocytes resulted in the induction of human albumin production (31). Attempts to induce immunoglobulin production (17,(32)(33)(34)(35)(36) have failed. However, fusion of two parental cells capable of producing immunoglobulin has permitted continued immunogiobulin production (11,17,32,34).…”

Section: Resultsmentioning

confidence: 99%

“…Attempts to induce immunoglobulin production (17,(32)(33)(34)(35)(36) have failed. However, fusion of two parental cells capable of producing immunoglobulin has permitted continued immunogiobulin production (11,17,32,34).…”

Section: Resultsmentioning

confidence: 99%

Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia.

Schwaber¹,

Rosen²

1978

The Journal of Experimental Medicine

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Somatic cell hybrid clones were isolated from the fusion of RPC 5,4 mouse myeloma cells and B lymphocytes from three patients with agammaglobulinemia. One patient had X-linked agammaglobulinemia; the remaining two patients had common varied agammaglobulinemia. All three patients had B lymphocytes which fail to secrete immunoglobulin. The hybrid nature of the clones was established by examination of metaphase chromosome spreads. Most of the clones from all three patients expressed surface immunoglobulin of mouse and human parental origin. Clones from two of the patients had fewer cells with surface Ig than hybrids from normal persons, while clones from the third patient had large numbers of surface Ig fluorescent cells. Most of the clones from all three patients synthesized and secreted human and mouse immunoglobulin. As determined by sodium dodecyl sulfate acrylamide gel electrophoresis of radioactively labeled proteins, clones from each of the patients produced human gamma, alpha, and mu-heavy chains. These studies demonstrate the presence of functional structural genes coding for human immunoglobulin heavy chains in B lymphocytes of patients with agammaglobulinemia. Further, they represent induction in the somatic cell hybrids of a gene product not expressed in the parental B lymphocytes.

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Selective hybrid cell formation by human lymphoid cells

Parkman

Hagemeijer

Merler

1973

Journal Cellular Physiology

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Subpopulations of human lymphoid cells are capable of spontaneously fusing with fibroblasts of human or murine origin to form humanhuman or human-mouse hybrid cells. These cells were present in thymus, spleen, and bone marrow. After fractionation on discontinuous bovine serum albumin gradients, the cells were found in the less dense layers of the gradient. Cells of fetal origin, except for bone marrow, fused spontaneously at a higher rate than those of adult origin. The highest rate of fusion was found with adult bone marrow cells. These lymphoid cells appear to be "thymus-derived' cells.

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Immunoglobulin G and Free Kappa-Chain Synthesis in Different Clones of a Hybrid Cell Line

Cited by 19 publications

References 17 publications

High serum-free light chain levels and their rapid reduction in response to therapy define an aggressive multiple myeloma subtype with poor prognosis

High serum-free light chain levels and their rapid reduction in response to therapy define an aggressive multiple myeloma subtype with poor prognosis

Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia.

Selective hybrid cell formation by human lymphoid cells

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