Immunogenicity of Human Amniotic Epithelial Cells After Transplantation Into Volunteers

Akle, Charles; Adinolfí, Matteo; Welsh, Ken I.; Leibowitz, Sidney; McColl, Ian

doi:10.1016/s0140-6736(81)91212-5

Cited by 430 publications

(263 citation statements)

References 3 publications

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“…Several studies reported an antiinflammatory effect of AM by reducing the expression of IL-1a and IL-1b proinflammatory cytokines 29 or by decreasing polymorphonuclear cell infiltration. 30 Immunorejection of AM has not been observed in clinical studies of AM 23,31 and cells of cryopreserved AM are expected to be dead after cryopreservation at -80°C in glycerol. 32 This suggests that AM immunogenicity seems to be low.…”

Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

Roux

Bodivit

Bartis

et al. 2015

Tissue Engineering Part A

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Stem cells may represent an excellent strategy to improve the healing of skin ulcers. Today the administration mode of stem cells to skin defects remains unsatisfactory. Delivering stem cells with topical treatments represents a new strategy and answering the patients' need. Mesenchymal stromal cells (MSC) have been shown to improve wound healing of cutaneous lesions and amniotic membrane (AM) is known to represent a natural scaffold for cells. The aim of this study is to develop a tissue-engineered product combining MSC and AM for clinical use. In this work we investigated whether the stromal matrix of intact human AM could constitute a scaffold for human MSC derived from either bone marrow (BM) or adipose tissue (AT). For this purpose, clinical-grade AM, MSC, and culture medium were used. We performed experiments of short-term adherence and proliferation for 15 days after the seeding of the cells. Morphological aspects and secretion profiles of MSC onto AM were studied, respectively, by scanning electron microscopy and Luminex analysis. Results demonstrated that the stromal matrix allow the adherence in much greater amount of MSC from BM or AT compared to 2D material. Experiments of proliferation showed that both kinds of MSC could proliferate on the stromal matrix and remain viable 15 days after the seeding of the cells. The 3D analysis of MSC culture demonstrated that both types of MSC invaded the stromal matrix and grew in multiple layers while retaining their fibroblastic morphology. By studying the secretion profile of MSC onto the stromal matrix, we found that both kinds of MSC secrete important cytokines and growth factors for wound healing of cutaneous lesions, such as vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor. In conclusion, these results suggest that the stromal matrix of AM seeded with MSC represents a bioactive scaffold that should be evaluated in patients with a nonhealing cutaneous wound.

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Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

Roux

Bodivit

Bartis

et al. 2015

Tissue Engineering Part A

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“…The amnion consists solely of epithelial cells, and thus not MSCs 58, but others have also noted stem cell characteristics in this population 59, although caution should be expressed since Miki et al 59 referenced an extraction technique first described by Akle et al 60 where they stated that “ The amniotic membrane thus obtained consisted of an epithelial monolayer on a basement membrane with an underlying collagen matrix containing a few fibroblasts .”(another example of how harvesting procedures can affect the interpretation of experimental outcomes). Nevertheless, the sub‐amnion is mesenchymal and does contain MSCs 13, 18, 57, 61.…”

Section: Are Wharton's Jelly Mscs Perivascular In Origin?mentioning

confidence: 99%

Concise Review: Wharton's Jelly: The Rich, but Enigmatic, Source of Mesenchymal Stromal Cells

Davies

Walker

Keating

2017

Stem Cells Translational Medicine

158

145

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The umbilical cord has become an increasingly used source of mesenchymal stromal cells for preclinical and, more recently, clinical studies. Despite the increased activity, several aspects of this cell population have been under‐appreciated. Key issues are that consensus on the anatomical structures within the cord is lacking, and potentially different populations are identified as arising from a single source. To help address these points, we propose a histologically based nomenclature for cord structures and provide an analysis of their developmental origins and composition. Methods of cell isolation from Wharton's jelly are discussed and the immunophenotypic and clonal characteristics of the cells are evaluated. The perivascular origin of the cells is also addressed. Finally, clinical trials with umbilical cord cells are briefly reviewed. Interpreting the outcomes of the many clinical studies that have been undertaken with mesenchymal stromal cells from different tissue sources has been challenging, for many reasons. It is, therefore, particularly important that as umbilical cord cells are increasingly deployed therapeutically, we strive to better understand the derivation and functional characteristics of the cells from this important tissue source. Stem Cells Translational Medicine 2017;6:1620–1630

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“…It has also been shown to stimulate granulation tissue and new vessel formation in chronic venous leg ulcers by producing angiogenic factors, as demonstrated by Faulk et al (2). The amnion does not express histocompatibility antigens and there is no evidence of immune rejection when amnion is implanted subcutaneously (9). These features make the amnion a perfect tissue graft for covering the artificially dissected vagina in patients with congenital absence of the vagina.…”

Section: Discussionmentioning

confidence: 97%

Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina

Bleggi-Torres¹,

Werner²,

Mj³

1997

Braz J Med Biol Res

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We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neoepithelium were collected for transmission electron microscopy analysis. The ultrastructural findings consisted of a lining of mature squamous epithelium indicating the occurrence of metaplasia of the amniotic epithelium into the vaginal epithelium. The cells were arranged in layers as in the normal vaginal epithelium, i.e., superficial, intermediate and deep layers. There were desmosomes and cytoplasmic intermediate cytokeratin filaments, as well as some remnant features of the previous amniotic epithelium. These findings suggest that human amniotic membrane is able to complete metaplasia into squamous cells but the mechanism of this cellular transformation is unknown.

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Immunogenicity of Human Amniotic Epithelial Cells After Transplantation Into Volunteers

Cited by 430 publications

References 3 publications

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

In VitroCharacterization of Patches of Human Mesenchymal Stromal Cells

Concise Review: Wharton's Jelly: The Rich, but Enigmatic, Source of Mesenchymal Stromal Cells

Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina

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