Immunogenicity and protective efficacy of an inactivated Newcastle disease virus vaccine encapsulated in poly-(lactic-co-glycolic acid) nanoparticles

Kumar, B.S. Ananda; Panickan, Sivasankar; Bindu, Suresh; Kumar, Vinod; Ramakrishnan, Saravanan; Sonal,; Shrivastava, Sameer; Dandapat, S.

doi:10.1016/j.psj.2023.102679

Cited by 2 publications

(1 citation statement)

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“…(2022) and Ananda Kumar et al. (2023) demonstrated that the NDV vaccine induced a robust immune response characterized by protective immunity, likely contributing to the observed protection in our study. Furthermore, the studies by Dimitrov et al.…”

Section: Discussionsupporting

confidence: 67%

Evaluation of the immune response of layer chickens to Newcastle disease virus vaccines using the new vaccination regimens

Geletu,

Robi

2024

Veterinary Medicine & Sci

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BackgroundThe study aimed to evaluate the immunological response of layer chickens to live Newcastle disease virus vaccine using a newly developed vaccine schedule administered via the ocular route, as well as assess the persistence of passive antibodies in layer chickens and the effectiveness of protection against strains of the virus.MethodsA total of 140‐day‐old Lohmann Brown chicks were randomly divided into seven groups, 20 chicks each. Groups 1–3 received a single eye instillation of the vaccine at ages 5, 26 and 54 days, respectively, whereas groups 4–6 received a double eye instillation. Group 7 served as non‐vaccinated control group. Ten days after immunization, samples were taken from hens that had received the vaccine at ages 15, 36 and 64, as well as from control chickens that had not received the vaccine at ages 5, 15, 21 and 31.ResultsA total of 10 serum samples from all chickens exhibited protective antibodies, and booster doses resulted in the highest haemagglutination inhibition titre. No significant change in antibody production was observed among layer hens (p > 0.05). The study found that the La Sota (GMT ± SD: 6.71 ± 4.96), La Sota (GMT ± SD: 8.00 ± 0.00) and thermostable I2 (GMT ± SD: 7.60 ± 6.02), vaccination schedules provided the maximum immune response in single eye instillation, whereas the HB1 (GMT ± SD: 7.11 ± 4.77), La Sota (GMT ± SD: 7.83 ± 5.76) and La Sota (GMT ± SD: 7.60 ± 6.02), combination was the second‐best vaccination schedule in double eye instillation. Furthermore, maternally‐derived antibodies were maintained up to 31 days of age, indicating the level of passive immunity prior to vaccination. Characteristic lesions, such as edematous and diphtheria mucosal membranes of the trachea, along with petechial and necrotic haemorrhages of the proventriculus, were observed during the necropsy of the birds that died from the challenged virus.ConclusionThis study suggests that subsequent live virus vaccine by ocular route immunization is required to effectively protect against velogenic viscerotropic Newcastle disease infection. The results also highlight the importance of developing effective vaccination schedules and routes to enhance immunity against ND in layer chickens.

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Section: Discussionsupporting

confidence: 67%