Immunogenic and protective efficacy of recombinant protein GtxA-N against<i>Gallibacterium anatis</i>challenge in chickens

Pedersen, Ida J.; Pors, Susanne Elisabeth; Skjerning, Ragnhild J. Bager; Nielsen, Søren Saxmose; Bojesen, Anders Miki

doi:10.1080/03079457.2015.1069259

Cited by 15 publications

(22 citation statements)

References 30 publications

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“…This vaccine formulation was adopted in study 1 in order to compare the response elicited by immunizing solely with FlfA + GtxA-N against the response elicited when G. anatis OMVs were also added to the vaccine formulation. Given that immunization with both FlfA [18,22] and GtxA-N [23] was previously reported to be effective in providing protection when co-administered with G. anatis OMVs or other adjuvants, and considering the well known adjuvant role exerted by the OMVs [17,20], the lack of protection observed in group 2 can most likely be attributed to the absence of an adjuvant in the vaccine formulation FlfA + GtxA-N.…”

Section: Discussionmentioning

confidence: 99%

“…The immunization regimes presented in study 1 include all possible combinations of G. anatis OMVs and the recombinant proteins FlfA and GtxA-N ( Figure 1). Although several of these immunogen combinations have been previously tested as vaccine candidates [18,[22][23][24][25], a systematic study including all possible combinations of these immunogens and HF-isolated G. anatis OMVs was not reported yet.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Additionally, the OMVs have been shown to act as a potent adjuvant when co-administered with other immunogens [17][18][19], further increasing the possible applications of vesicles in vaccine development [20]. Our group previously reported that G. anatis secretes OMVs [21], and that these vesicles, in combination with the conserved proteins FlfA or the N-terminal of the GtxA toxin [22][23][24], are effective in preventing the development of lesions associated with G. anatis infections when administered as immunogens [18,25]. FlfA is a conserved fimbrial protein involved in G. anatis adhesion and pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Hydrostatic Filtration Enables Large-Scale Production of Outer Membrane Vesicles That Effectively Protect Chickens against Gallibacterium anatis

et al. 2020

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Gallibacterium anatis is a Gram-negative opportunistic avian pathogen representing an emerging threat to poultry meat and egg production worldwide. To date, no vaccine able to effectively prevent the morbidity associated with G. anatis infections has been developed yet. Our group previously reported that inoculation of different combinations of G. anatis outer membrane vesicles (OMVs), FlfA and GtxA-N proteins is effective in preventing lesions caused by G. anatis infections in layer chickens. Here we report the testing of the efficacy as vaccine prototypes of G. anatis OMVs isolated by hydrostatic filtration, a simple technique that allows the cost-effective isolation of high yields of OMVs. Layer chickens were immunized with OMVs alone or in combination with FlfA and/or GtxA-N proteins. Subsequent challenge with a heterologous G. anatis strain showed that immunization with OMVs alone could significantly reduce the lesions following a G. anatis infection. A second study was carried out to characterize the dose-response (0.25, 2.5 and 25 µg) relationship of G. anatis OMVs as immunogens, showing that 2.5 μg of OMVs represent the optimal dose to elicit protection in the immunized animals after a similar challenge. Additionally, administration of ≥2.5 μg of G. anatis OMVs induced specific IgY titers and possibly vertical transfer of immunity.

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrostatic Filtration Enables Large-Scale Production of Outer Membrane Vesicles That Effectively Protect Chickens against Gallibacterium anatis

et al. 2020

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“…(2004) performed a study with a detailed experimental design, including sequential killing of birds between 3 and 24 h postinfection, after intraperitoneal or intravenous infection. Intraperitoneal infection was applied by the same research group in other studies, in order to mimic a peritoneal infection due to ascending bacteria from the cloaca via the oviduct, a hypothesis well known from E. coli infections in layers (Bager et al ., 2013; Pedersen et al ., 2015; Pors et al ., 2016). Testing certain deletion mutants, and/or the efficacy of recombinant proteins to be used as vaccine candidates, fibrinous and purulent peritonitis were noticed, similar to previous studies and expected due to the route of infection.…”

Section: Introductionmentioning

confidence: 99%

Commensal or pathogen – a challenge to fulfil Koch’s Postulates

Heß

2017

British Poultry Science

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ABSTRACT1. Infectious diseases have a large impact on poultry health and economics. Elucidating the pathogenesis of a certain disease is crucial to implement control strategies.2. Multiplication of a pathogen and its characterisation in vitro are basic requirements to perform experimental studies. However, passaging of the pathogen in vitro can influence the pathogenicity, a process targeted for live vaccine development, but limits the reproduction of clinical signs.3. Numerous factors can influence the outcome of experimental infections with some importance on the pathogen, application route and host as exemplarily outlined for Histomonas meleagridis, Gallibacterium anatis and fowl aviadenoviruses (FAdVs).4. In future, more comprehensive and detailed settings are needed to obtain as much information as possible from animal experiments. Processing of samples with modern diagnostic tools provides the option to closely monitor the host–pathogen interaction.

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“…Some of them have been shown to have immunogenic potential against homologous challenge. Among all the recombinant proteins tested, the N terminus of Gallibacterium toxin A (GtxA-N), which is a well-conserved RTX (repeats in toxin) toxin produced by G. anatis that has both hemolytic and leukotoxic activities (17,18), showed the highest antibody titers in the sera of chickens infected with G. anatis (16). GtxA-N is widespread among Gallibacterium strains of different origins (17,19) and therefore represents a promising candidate for the induction of protective immunity.…”

mentioning

confidence: 99%

Specific Chicken Egg Yolk Antibody Improves the Protective Response against Gallibacterium anatis Infection

et al. 2019

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Gallibacterium anatisis a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against aG. anatisinfection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus ofGallibacteriumtoxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth ofG. anatisin brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated inG. anatis-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera ofG. anatis-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response againstG. anatisinfection in chicks.

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Immunogenic and protective efficacy of recombinant protein GtxA-N againstGallibacterium anatischallenge in chickens

Cited by 15 publications

References 30 publications

Hydrostatic Filtration Enables Large-Scale Production of Outer Membrane Vesicles That Effectively Protect Chickens against Gallibacterium anatis

Hydrostatic Filtration Enables Large-Scale Production of Outer Membrane Vesicles That Effectively Protect Chickens against Gallibacterium anatis

Commensal or pathogen – a challenge to fulfil Koch’s Postulates

Specific Chicken Egg Yolk Antibody Improves the Protective Response against Gallibacterium anatis Infection

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