Immunofluorescent staining with antibodies to factor VIII, fibronectin, and collagenous basement membrane protein in normal human skin and port wine stains

Abstract

Specific antibodies directed against three important components of the vessel wall (collagenous basement membrane protein-type IV collagen, fibronectin, and factor VIII) were used to study and compare the distribution of these proteins in normal skin and port wine stains. Collagenous basement membrane protein was localized to the basement membrane and basal lamina zones of blood vessels, appendages, arrector pili muscles, endoneurium and perineurium, and the dermoepidermal junction of both port wine stains and normal skin. Vessels of the port wine stain as well as those of normal skin showed a similar narrow uniform homogeneous line of fluorescence. Granular endothelial staining was seen in the blood vessels of both normal skin and port wine stains. The distribution of fibronectin was that of a low-intensity punctate pattern situated in the subendothelial region of both port wine stain and normal vessels and in the basement membrane zones of hair follicles, endoneurium and perineurium, and the dermoepidermal junction.

Immunohistological Evaluation of Endothelial Markers and Basement Membrane Components in Port-Wine Stains

“…Concerning the staining pattern, our results were essentially consistent with the previous report by Finley et al [14], who examined PWSs by immunofluorescence us ing antibodies against Factor VIII RA, fibronectin and type IV collagen. In addi tion to Finley et al, we found thickening of the BM around dilated vessels in the lesions.…”

Comparison of Efficacy Between Hemoporfin Photodynamic Therapy and Pulsed Dye Laser in the Treatment of Port‐Wine Stains With Different Vascular Patterns: A Retrospective Study

“…Conversely, HMME‐PDT relies on administering a photosensitizer, which generates cytotoxic reactive oxygen species that subsequently occlude and destroy the target blood vessels upon activation by specific wavelength irradiation [14]. Given the significant histopathological variability observed in PWS [15], performing pathological examination on every patient is impractical. Dermoscopy is a noninvasive and reproducible examination method that correlates closely with the histopathology of lesions [16].…”

The effect of ambient temperature on capillary vascular malformations

“…Previous studies have suggested that abnormal innervation may be a causative factor of CM, rather than vessel wall abnormality, 25 with several studies noting that both autonomic and sensory nerves appear to be deficient within CM skin 14–16 . As sensory nerves are important for growth regulation, 26 it has been postulated that a lack of neural innervation and subsequent loss of vessel tone lead to the progression of the malformation.…”