Immunoexpression of GADD45β in the myocardium of newborns experiencing perinatal hypoxia

Simões, Mona Adalgisa; Pabis, Francisco Cesar; Freitas, Ana Karyn Ehrenfried de; Azevedo, Marina Luise Viola de; Ronchi, Daiane Cristine Martins; Noronha, Lúcia de

doi:10.1016/j.prp.2016.12.011

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…After scanning, the software generated images in the photomicrograph format, which were analyzed using the image analysis software Image-Pro Plus® (Rockville, MD, USA). The immunostained area of each photomicrograph was measured, including both nuclear and cytoplasmic areas, and was expressed in square micrometers, and the mean was calculated for each case and transformed into percentage by half-magnification field (HMF) by dividing by the constant 115.226,1 μm 2 and multiplying by 100 for subsequent statistical analysis [19].…”

Section: Methodsmentioning

confidence: 99%

Tissue expression of retinoic acid receptor alpha and CRABP2 in metastatic nephroblastomas

Percicote

Mardegan

Gugelmim³

et al. 2018

Diagn Pathol

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Background: Nephroblastoma or Wilms tumor is the most frequent kidney cancer in children and accounts for 98% of kidney tumors in this age group. Despite favorable prognosis, a subgroup of these patients progresses to recurrence and death. The retinoic acid (RA) pathway plays a role in the chemoprevention and treatment of tumors due to its effects on cell differentiation and its antiproliferative, anti-oxidant, and pro-apoptotic activities. Reports describe abnormal cellular retinoic acid-binding protein 2 (CRABP2) expression in neoplasms and its correlation with prognostic factors and clinical and pathological characteristics. The aim of this study was to evaluate the immunohistochemical expression of retinoic acid receptor alpha (RARA) and CRABP2 in paraffin-embedded samples of nephroblastomas via semiquantitative and quantitative analyses and to correlate this expression with prognostic factors. Methods: Seventy-seven cases of nephroblastomas were selected from pediatric oncology services. The respective medical records and surgical specimens were reviewed. Three representative tumor samples and one non-tumor renal tissue sample were selected for the preparation of tissue microarrays (TMA). The Allred scoring system was used for semiquantitative immunohistochemical analyses, whereas a morphometric analysis of the stained area was employed for quantitative evaluation. The nonparametric Mann-Whitney test was used for comparisons between two groups, while the nonparametric Kruskal-Wallis test was used to compare three or more groups. Results: Immunopositivity for RARA and CRABP2 was observed in both the nucleus and cytoplasm. All histological components of the nephroblastoma (blastema, epithelium, and stroma) were positive for both markers. RARA, based on semiquantitative analyses, and CRABP2, bases on quantitative analyses, exhibited increased immunohistochemical expression in patients with metastasis, with p values of 0.0247 and 0.0128, respectively. These findings were similar to the results of the quantitative analysis of RARA expression, showing greater immunopositivity in tumor samples of patients subjected to pre-surgical chemotherapy. No significant correlation was found with the other variables studied, such as disease stage, anaplasia, risk group, histological type, nodal involvement, and clinical evolution. Conclusions: Semiquantitative and quantitative analyses of the markers RARA and CRABP2 indicate their potential as biomarkers for tumor progression and their participation in nephroblastoma tumorigenesis.

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Section: Methodsmentioning

confidence: 99%

Tissue expression of retinoic acid receptor alpha and CRABP2 in metastatic nephroblastomas

Percicote

Mardegan

Gugelmim³

et al. 2018

Diagn Pathol

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Downregulation of IGF2 expression in third trimester placental tissues from Zika virus infected women in Brazil

Suzukawa

Zanluca

Jorge³

et al. 2020

Journal of Infection

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Objectives: Screening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be involved in ZIKV congenital pathogenesis. Methods: Transcriptome data from placental tissues of pregnant women naturally infected with Zika virus during the third trimester were compared to those from women who tested negative for Zika infection. The findings were validated using both a cell culture model and an immunohistochemistry/morphological analysis of naturally infected placental tissues. Results: Transcriptome analysis revealed that Zika virus infection induces downregulation of insulin-like growth factor II (IGF2) gene, an essential factor for fetal development. The Caco-2 cell culture model that constitutively expresses IGF2 was used for the transcriptome validation. Asiatic and African Zika virus strains infection caused downregulated IGF2 gene expression in Caco-2 cells, whereas other flaviviruses, such as dengue serotype 1, West Nile and wild-type yellow fever viruses, had no effect on this gene expression. Immunohistochemical assays on decidual tissues corroborated our transcriptome analysis, showing that IGF2 is reduced in the decidua of Zika virus-infected women. Conclusions: Our results draw attention to IGF2 modulation in uterine tissues, and this finding is expected to support future studies on strategies to ameliorate the harmful effects of Zika virus infection during pregnancy.

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Immunoexpression of GADD45β in the myocardium of newborns experiencing perinatal hypoxia

Cited by 2 publications

References 17 publications

Tissue expression of retinoic acid receptor alpha and CRABP2 in metastatic nephroblastomas

Tissue expression of retinoic acid receptor alpha and CRABP2 in metastatic nephroblastomas

Downregulation of IGF2 expression in third trimester placental tissues from Zika virus infected women in Brazil

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