Immunodiagnosis of human neurocysticercosis by using semi-purified scolex antigens from Taenia solium cysticerci

Neto, Francesco Iudici; Pianetti-Filho, Geraldo; Araújo, Ricardo Nascimento; Nascimento, Evaldo

doi:10.1590/s0037-86822007000200004

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…[15] The cross reaction was partly due to the antigenic variation in the developmental stages of C. cellulosae and even strain variations in certain Taenia species. As the quality of the cysticercus antigen used in the methods played a major role in the specificity and sensitivity of an immunodiagnostic test, stress was placed on fractionation, purification, and characterization of the antigens.…”

Section: Antigens Of the Cysticercimentioning

confidence: 99%

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Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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Neurocysticercosis (NCC) is difficult to diagnose clinically because of its varied clinical presentation. However, an accurate diagnosis is possible only after suspicion on epidemiological grounds, proper interpretation of the clinical data, analysis of the findings on imaging studies, and specific immunological tests on the serum and cerebrospinal fluid (CSF). The diagnosis of NCC by any single parameter thus continues to remain difficult. In the past, detection of NCC was based on autopsy studies and histological confirmation. In recent times, the advent of imaging methods such as computed tomography and magnetic resonance imaging have provided excellent non-invasive tools for easy detection of NCC. Nevertheless, an imaging technique of the brain, although useful, is not considered as a gold standard for the diagnosis of NCC. Serological tests are being increasingly used in adjunct with imaging techniques, to aid the diagnosis of NCC. Immunodiagnostic techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum and CSF. Currently, many of the immunodiagnostic tests, including the enzyme-linked immunosorbent assay and enzyme immunotransfer blot, use purified native antigens for the immunodiagnosis of NCC. Nevertheless, the main problem with the use of native cysticercal antigens is that the native proteins often show cross reactions with sera from humans infected with other parasites. The preparation of native antigens also demand a constant supply of parasitic material from the intermediate host pig. In order to overcome the problems in using native antigens, the recombinant antigens or synthetic peptides, which can be produced under stable conditions, are being evaluated for the serodiagnosis of NCC.

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Section: Antigens Of the Cysticercimentioning

confidence: 99%

“…However, tests using these unpurified native parasite antigens show moderate sensitivities and relatively poor specificities. [91415] In the recent past, improved protein-purification techniques and research on the antigenic properties of cyst fluid and surface proteins have led to the development of better serological tools. [16–18]…”

Section: Introductionmentioning

confidence: 99%

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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“…Our study results indicate that different parasites have variant diagnostic antigens, therefore they can be used for specific and differential detections. For example, the proteins of T. solium cysticerci or C. cellulosae with molecular weights of 100, 95 or 26 kDa have been used in confirmatory tests for neurocysticercosis-positive cases [49]. Meanwhile, the 98 kDa antigen of A. cantonensis as a candidate antigen can be used for the diagnosis of angiostrongyliasis [43].…”

Section: Discussionmentioning

confidence: 99%

“…Semi-purification of antigens was performed following a protocol reported previously with some modifications [49]. Briefly, SDS-PAGE gel electrophoresis was performed to separate the various parasite antigens.…”

Section: Methodsmentioning

confidence: 99%

A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases

Chen

et al. 2012

PLoS Negl Trop Dis

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BackgroundFood-borne helminthiases (FBHs) have become increasingly important due to frequent occurrence and worldwide distribution. There is increasing demand for developing more sensitive, high-throughput techniques for the simultaneous detection of multiple parasitic diseases due to limitations in differential clinical diagnosis of FBHs with similar symptoms. These infections are difficult to diagnose correctly by conventional diagnostic approaches including serological approaches.Methodology/Principal FindingsIn this study, antigens obtained from 5 parasite species, namely Cysticercus cellulosae, Angiostrongylus cantonensis, Paragonimus westermani, Trichinella spiralis and Spirometra sp., were semi-purified after immunoblotting. Sera from 365 human cases of helminthiasis and 80 healthy individuals were assayed with semi-purified antigens by both a protein microarray and the enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity and simplicity of each test for the end-user were evaluated. The specificity of the tests ranged from 97.0% (95% confidence interval (CI): 95.3–98.7%) to 100.0% (95% CI: 100.0%) in the protein microarray and from 97.7% (95% CI: 96.2–99.2%) to 100.0% (95% CI: 100.0%) in ELISA. The sensitivity varied from 85.7% (95% CI: 75.1–96.3%) to 92.1% (95% CI: 83.5–100.0%) in the protein microarray, while the corresponding values for ELISA were 82.0% (95% CI: 71.4–92.6%) to 92.1% (95% CI: 83.5–100.0%). Furthermore, the Youden index spanned from 0.83 to 0.92 in the protein microarray and from 0.80 to 0.92 in ELISA. For each parasite, the Youden index from the protein microarray was often slightly higher than the one from ELISA even though the same antigen was used.Conclusions/SignificanceThe protein microarray platform is a convenient, versatile, high-throughput method that can easily be adapted to massive FBH screening.

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“…As proteínas do escólex são bastante específicas (100%) e a sensibilidade variou de 89% a 100% (20,45) . As frações proteicas purificadas por eletroeluição, a partir do gel de poliacrilamida, apresentaram alta sensibilidade: 100% para o antígeno de 100 kDa e 95% para as frações de 26 kDa e 95 kDa (45) .…”

Section: Antígeno Homólogounclassified

Diagnóstico laboratorial da neurocisticercose: revisão e perspectivas

Togoro¹,

Souza²,

Sato³

2012

J. Bras. Patol. Med. Lab.

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A neurocisticercose é causada por Cysticercus cellulose, a forma larval de Taenia solium, quando este se aloja no sistema nervoso central. O seu diagnóstico é realizado com base em dados clínicos, epidemiológicos, demonstração do agente etiológico pelas técnicas de imagem e testes laboratoriais. No presente estudo, apresentamos uma revisão do diagnóstico laboratorial, com ênfase no desempenho dos testes para pesquisa de anticorpos específicos e detecção de antígenos circulantes, utilização de antígeno homólogo ou heterólogo, nativo e recombinante, bem como a aplicação de métodos moleculares. resumo unitermos Neurocisticercose Taenia solium Cysticercus cellulosaeDiagnóstico laboratorial abstract Neurocysticercosis is caused by Cysticercus cellulosae, the larval form of Taenia solium, when it lodges in the central nervous system. The diagnosis of neurocysticercosis is based on clinical and epidemiological data, neuroimaging findings of etiological agent and serologic test results. Herein we present a review of clinical diagnosis, emphasizing test performance for specific antibody and antigen detection, the use of homologous or heterologous antigen, native and recombinant antigens as well as the application of molecular methods.

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Immunodiagnosis of human neurocysticercosis by using semi-purified scolex antigens from Taenia solium cysticerci

Cited by 10 publications

References 33 publications

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases

Diagnóstico laboratorial da neurocisticercose: revisão e perspectivas

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