Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope

Rodrigues, Marcella Rezende; Santos, Lucas Magno Oliveira; Miyazaki, Carolina K.; Martins, Vívian T.; Ludolf, Fernanda; Kursancew, Amanda Christine Silva; Ramos, Fernanda F.; Dias, Daniel Lucas Santos; Oliveira, Jamil S.; Vieira, Paula Melo Abreu; Roatt, Bruno Mendes; Machado-de-Ávila, Ricardo Andrez; Gonçalves, Denise Utsch; Menezes‐Souza, Daniel; Coelho, Eduardo Antônio Ferraz; Duarte, Mariana C.

doi:10.1016/j.jim.2019.112641

Cited by 14 publications

(8 citation statements)

References 51 publications

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“…In symptomatic visceral leishmaniasis, there is an increased antibody response, both in dogs and in humans, and recombinant antigen-based assays can thus be used [33][34][35]. The challenge is asymptomatic leishmaniasis, in which we face a low serology against the protozoan, the tests showing a reduced sensitivity, as well as a cross-reactivity with other pathologies such as tuberculosis, Chagas disease, or malaria, which reduces the tests' specificity [36,37]. Recently, the rLiHyQ protein was identified in the antigens of the protozoan leishmania; this was cloned and used in ELISA experiments for determining the diagnosis in both human and canine leishmaniasis, showing sensitivity and specificity even in HIV-coinfected patients [19].…”

Section: Diagnosis In Humans Leishmaniasismentioning

confidence: 99%

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

Martinescu¹

2023

Preprint

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Laboratory diagnosis of leishmaniasis remains a major challenge, demonstrating variable efficacy in detecting infected mammalian hosts. Thus, there is a great need for the accuracy of the identification of appropriate antigens to also improve diagnostic tests. This review brings to the fore the sensitivity of the balance in canine and human leishmaniasis, which greatly influences the progression of the disease and especially the diagnostic methods. The problem of diagnosis arises in asymptomatic leishmaniasis, since in these conditions the methods considered as reference in the diagnosis of leishmaniasis no longer show certainty, being influenced for the most part by the immune response of the host, which is different according to the presence of other associated diseases or even according to the breed, when we talk about dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, with the need for new methods adapted to the immunological status of the host.

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Section: Diagnosis In Humans Leishmaniasismentioning

confidence: 99%

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

Martinescu¹

2023

Preprint

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“…In symptomatic visceral leishmaniasis, there is an increased antibody response, both in dogs and in humans, and recombinant antigen-based assays can thus be used ( Dhom-Lemos et al., 2019 ; Lévêque et al., 2020 ; Pereira et al., 2020 ). The challenge is asymptomatic leishmaniasis, in which we face a low serology against the protozoan, the tests showing a reduced sensitivity, as well as a cross-reactivity with other pathologies such as tuberculosis, Chagas disease, or malaria, which reduces the tests’ specificity ( Daltro et al., 2019 ; Rodrigues et al., 2019 ). Recently, the rLiHyQ protein was identified in the antigens of Leishmania spp.…”

Section: Diagnosis In Human Leishmaniasismentioning

confidence: 99%

The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research

Ivănescu,

Andronic,

Grigore-Hristodorescu

et al. 2023

Front. Cell. Infect. Microbiol.

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Leishmaniasis is a widespread but still underdiagnosed parasitic disease that affects both humans and animals. There are at least 20 pathogenic species of Leishmania, most of them being zoonotic. The diagnosis of leishmaniasis remains a major challenge, with an important role being played by the species of parasites involved, the genetic background, the immunocompetence of the host. This paper brings to the fore the sensitivity of the balance in canine and human leishmaniasis and addresses the importance of the host’s immune response in establishing a correct diagnosis, especially in certain cases of asymptomatic leishmaniasis, or in the situation the host is immunosuppressed or acquired leishmaniasis through vertical transmission. The methods considered as a reference in the diagnosis of leishmaniasis no longer present certainty, the diagnosis being influenced mostly by the immune response of the host, which differs according to the presence of other associated diseases or even according to the breed in dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, requiring new diagnostic methods adapted to the immunological state of the host.

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“…Other recombinant proteins, such as cytochrome c oxidase, putative IgE histamine releasing factor, prohibitin, eukaryotic initiation factor 5a, cathepsin L-like peptide and small myristoylated protein-3, as well as hypothetical proteins, were evaluated in preliminary studies demonstrating potential as candidates for TL-immunodiagnosis, and so more studies are desirable [27,29,30,93]. Some promising synthetic peptides have been identified and employed in ELISA.…”

Section: Plos Onementioning

confidence: 99%

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

et al. 2021

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Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario for TL-diagnosis. We performed a worldwide systematic review to identify antigenic targets that have been evaluated for the main clinical forms of TL, such as cutaneous (CL) and mucosal (ML) leishmaniasis. Included were original studies evaluating the sensitivity and specificity of immunological tests for human-TL, CL and/or ML diagnosis using purified or recombinant proteins, synthetic peptides or polyclonal or monoclonal antibodies to detect Leishmania-specific antibodies or antigens. The review methodology followed PRISMA guidelines and all selected studies were evaluated in accordance with QUADAS-2. Thirty-eight original studies from four databases fulfilled the selection criteria. A total of 79 antigens were evaluated for the detection of antibodies as a diagnostic for TL, CL and/or ML by ELISA. Furthermore, three antibodies were evaluated for the detection of antigen by immunochromatographic test (ICT) and immunohistochemistry (IHC) for CL-diagnosis. Several antigenic targets showed 100% of sensitivity and specificity, suggesting potential use for TL-diagnosis in its different clinical manifestations. However, a high number of proof-of-concept studies reinforce the need for further analysis aimed at verifying true diagnostic accuracy in clinical practice.

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Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope

Cited by 14 publications

References 51 publications

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

The Immune Response in Canine and Human Leishmaniasis and How This Influences the Diagnosis- a Review and Assessment of Recent Research

The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research

Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review

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