Immunodetection of the toxic portion of Vip3A reveals differential temporal regulation of its secretion among<i>Bacillus thuringiensis</i>strains

Argôlo-Filho, Ronaldo Costa; Loguercio, Leandro Lopes

doi:10.1111/jam.13775

Cited by 3 publications

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“…This protein is synthesized during the vegetative growth phase and is secreted into the medium (Estruch et al ., 1996). However, the secretion of Vip3Aa is differentially regulated among Bt strains, and the duration of the presence of these proteins in the supernatant is variable (Cai et al ., 2006; Argôlo‐Filho and Loguercio, 2018). In addition, Vip3Aa is stabilized and protected by the P20 chaperone protein, allowing it to accumulate after exponential growth (Shi et al ., 2006; Yu et al ., 2011).…”

Section: Introductionmentioning

confidence: 99%

Nutrient conditions determine the localization of Bacillus thuringiensis Vip3Aa protein in the mother cell compartment

Wang

Gan

Wang

et al. 2020

Microbial Biotechnology

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Vip3Aa was first identified as a protein secreted during the vegetative growth phase of Bacillus thuringiensis (Bt) bacteria and which shows high insecticidal toxicity against lepidopteran insect pests (Estruch et al., 1996). Bt strains formulated as bio-insecticides only had low amounts of Vip3Aa secreted to the medium. Here, we report that Vip3Aa proteins produced by three different Bt strains, including an industrial strain, were indeed not secreted to the culture solution when grown in sporulation medium, but were retained in the mother cell compartment. In order to further investigate the Vip3Aa secretion and location, we grew the strains in rich medium. We found that in rich medium, a fraction of Vip3Aa was secreted, suggesting that Vip3Aa secretion is nutrient-dependent. Regardless of the growth conditions, we found that Vip3Aa retained in cell pellets exhibited high toxicity against Spodoptera frugiperda larvae. Hence, we speculate that the accumulation of Vip3Aa protein in the mother cell compartment under sporulation conditions could still be used as an efficient strategy for industrial production in commercial Bt strains.

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Section: Introductionmentioning

confidence: 99%

Nutrient conditions determine the localization of Bacillus thuringiensis Vip3Aa protein in the mother cell compartment

Wang

Gan

Wang

et al. 2020

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

Immunodetection of the toxic portion of Vip3A reveals differential temporal regulation of its secretion amongBacillus thuringiensisstrains

Abstract: Immunoassays based on the developed anti-Vip3A antibody can be useful in a variety of basic studies. This method can be also coupled with toxicity assays on target insects, for more efficient screening methods of novel Bt strains/toxins with biocontrol applicability.

Cited by 3 publications

References 50 publications

Nutrient conditions determine the localization of Bacillus thuringiensis Vip3Aa protein in the mother cell compartment

Nutrient conditions determine the localization of Bacillus thuringiensis Vip3Aa protein in the mother cell compartment

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