Immunocytological study of phlyctenular eye disease

Abstract

Scrapings from phlyctens and conjunctiva of 12 patients with phlyctenular keratoconjunctivitis were studied using OKT4-Leu3a, OKT8, B1, BA1, S-100 and HLA-DR monoclonal antibodies. T-lymphocytes were present in both conjunctival and phlyctenular scrapings. OKT4-Leu3a positive cells outnumbered the OKT8 positive cells in both conjunctival and phlyctenular scrapings. B1 and BA1 positive cells were absent from the conjunctival scrapings, but were present in the phlyctenular scrapings. S-100 positive cells were present in both conjunctival and phlyctenular scrapings. However, they were very few in the conjunctival scrapings. Most of the cells in both conjunctival and phlyctenular scrapings were HLA-DR positive. These findings support the hypothesis that cell mediated immunity is responsible for the pathogenesis of phlyctenular eye disease.

Pediatric Phlyctenular Keratoconjunctivitis at a Tertiary Care Center in the United States

“…4,18,30,31,39 However, this cohort included a male predominance. Historically, PKC occurrence has been reported to be highest in the spring, 31,40 and perhaps this might be due to allergic associations. By contrast, the most frequent season for diagnosis in this study was the fall.…”

Pediatric Phlyctenular Keratoconjunctivitis at a Tertiary Care Center in the United States

“…4,18,30,31,39 However, this cohort included a male predominance. Historically, PKC occurrence has been reported to be highest in the spring, 31,40 and perhaps this might be due to allergic associations. By contrast, the most frequent season for diagnosis in this study was the fall.…”

Phenotypic characterization of inflammatory cells in phlyctenular eye disease

“…In a previous study of phlyctenular scrapings we showed that helper/inducer T-cells outnumbred suppressor/cytotoxic T-cells [19]. Very few Blymphocytes and small numbers of plasma cells, the majority expressing IgA, were found in the stromal inflammatory infiltrate, indicating that humoral immune mechanisms are of minor importance in the pathogenesis of the condition.…”

Pediatric Phlyctenular Keratoconjunctivitis at a Tertiary Care Center in the United States

“…4,18,30,31,39 However, this cohort included a male predominance. Historically, PKC occurrence has been reported to be highest in the spring, 31,40 and perhaps this might be due to allergic associations. By contrast, the most frequent season for diagnosis in this study was the fall.…”

Phenotypic characterization of inflammatory cells in phlyctenular eye disease

“…In a previous study of phlyctenular scrapings we showed that helper/inducer T-cells outnumbred suppressor/cytotoxic T-cells [19]. Very few Blymphocytes and small numbers of plasma cells, the majority expressing IgA, were found in the stromal inflammatory infiltrate, indicating that humoral immune mechanisms are of minor importance in the pathogenesis of the condition.…”

Pediatric Phlyctenular Keratoconjunctivitis at a Tertiary Care Center in the United States

“…4,18,30,31,39 However, this cohort included a male predominance. Historically, PKC occurrence has been reported to be highest in the spring, 31,40 and perhaps this might be due to allergic associations. By contrast, the most frequent season for diagnosis in this study was the fall.…”

Phenotypic characterization of inflammatory cells in phlyctenular eye disease

“…In a previous study of phlyctenular scrapings we showed that helper/inducer T-cells outnumbred suppressor/cytotoxic T-cells [19]. Very few Blymphocytes and small numbers of plasma cells, the majority expressing IgA, were found in the stromal inflammatory infiltrate, indicating that humoral immune mechanisms are of minor importance in the pathogenesis of the condition.…”